RiceWiki - User contributions [en]

Os10g0404500

2014-06-04T13:26:53Z

Xddjhh: /* Annotated Information */

Please input one-sentence summary here.

==Sucrose Transporter Gene==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(reference <ref name="ref1" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref2" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(reference <ref name="ref3" /> and reference <ref name="ref4" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref2" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref2" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref5" />).. "]]

==Labs working on this gene==
*Univ Minnesota, Dept Plant Biol, Biol Sci Ctr 250, 1445 Gortner Ave, St Paul, MN 55108 USA.
*CSIRO Plant Ind, Canberra, ACT 2601, Australia.
*Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China
*Natl Agr Res Ctr, Dept Rice Res, Niigata 9430193, Japan

==References==
<references>
<ref name="ref1">Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
<ref name="ref2">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref3">Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
<ref name="ref4">Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>
<ref name="ref5">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:26:15Z

Xddjhh: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(reference <ref name="ref1" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref2" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(reference <ref name="ref3" /> and reference <ref name="ref4" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref2" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref2" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref5" />).. "]]

==Labs working on this gene==
*Univ Minnesota, Dept Plant Biol, Biol Sci Ctr 250, 1445 Gortner Ave, St Paul, MN 55108 USA.
*CSIRO Plant Ind, Canberra, ACT 2601, Australia.
*Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China
*Natl Agr Res Ctr, Dept Rice Res, Niigata 9430193, Japan

==References==
<references>
<ref name="ref1">Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
<ref name="ref2">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref3">Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
<ref name="ref4">Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>
<ref name="ref5">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:26:02Z

Xddjhh: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(reference <ref name="ref1" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref2" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(reference <ref name="ref3" /> and reference <ref name="ref4" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref2" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref2" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref5" />).. "]]

==Labs working on this gene==
*Univ Minnesota, Dept Plant Biol, Biol Sci Ctr 250, 1445 Gortner Ave, St Paul, MN 55108 USA.
*CSIRO Plant Ind, Canberra, ACT 2601, Australia.
*Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China
*Natl Agr Res Ctr, Dept Rice Res, Niigata 9430193, Japan
*

==References==
<references>
<ref name="ref1">Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
<ref name="ref2">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref3">Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
<ref name="ref4">Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>
<ref name="ref5">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:24:43Z

Xddjhh: /* Labs working on this gene */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(reference <ref name="ref1" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref2" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(reference <ref name="ref3" /> and reference <ref name="ref4" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref2" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref2" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref5" />).. "]]

==Labs working on this gene==
*Univ Minnesota, Dept Plant Biol, Biol Sci Ctr 250, 1445 Gortner Ave, St Paul, MN 55108 USA.
*CSIRO Plant Ind, Canberra, ACT 2601, Australia.
*Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China

==References==
<references>
<ref name="ref1">Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
<ref name="ref2">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref3">Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
<ref name="ref4">Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>
<ref name="ref5">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:16:57Z

Xddjhh: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(reference <ref name="ref1" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref2" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(reference <ref name="ref3" /> and reference <ref name="ref4" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref2" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref2" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref5" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
<ref name="ref2">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref3">Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
<ref name="ref4">Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>
<ref name="ref5">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:15:19Z

Xddjhh: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(reference <ref name="ref1" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref2" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(reference <ref name="ref3" /> and reference <ref name="ref4" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref2" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref2" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref5" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref2">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>
3.Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
4.Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
5.Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:12:42Z

Xddjhh: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(reference <ref name="ref3" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(reference <ref name="ref4" /> and (from reference <ref name="ref5" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref1" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref2" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref2">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>
3.Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
4.Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
5.Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:12:07Z

Xddjhh: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides(from reference <ref name="ref3" /> ).
.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves(from reference <ref name="ref4" /> and (from reference <ref name="ref5" />).
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref1" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref2" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref2">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>
3.Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
4.Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
5.Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:09:06Z

Xddjhh: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref1" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref2" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref2">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>
3.Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
4.Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
5.Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T13:08:18Z

Xddjhh: /* References */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref1" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref2" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1">Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232.</ref>
<ref name="ref2">Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.</ref>
<ref name="ref3">Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref>
<ref name="ref4">Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref>
<ref name="ref5">Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:49:34Z

Xddjhh: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.
[[File:SUT_1.jpg|center|thumb|250x150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_2.jpg|center|thumb|250x150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref1" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref2" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:46:57Z

Xddjhh: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.
[[File:SUT_1.jpg|center|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_2.jpg|center|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref1" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_5.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref2" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

File:SUT 5.jpg

2014-06-04T12:46:42Z

Xddjhh: FCAS

FCAS

Os10g0404500

2014-06-04T12:44:50Z

Xddjhh: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.
[[File:SUT_1.jpg|center|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_2.jpg|center|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
[[File: SUT_3.jpg|right|thumb|150px|" Test function in yeast(from reference <ref name="ref1" />).. "]]

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
[[File: SUT_4.jpg|right|thumb|150px|" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference <ref name="ref2" />).. "]]

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

File:SUT 4.jpg

2014-06-04T12:44:39Z

Xddjhh: FACS

FACS

File:SUT 3.jpg

2014-06-04T12:35:33Z

Xddjhh: test function

test function

Os10g0404500

2014-06-04T12:33:08Z

Xddjhh: /* Evolution */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_1.jpg|left|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]
[[File:SUT_2.jpg|right|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
We can express the ''OsSUT'' gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:24:58Z

Xddjhh: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_1.jpg|left|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]
[[File:SUT_2.jpg|right|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:24:06Z

Xddjhh: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.
[[File:SUT_1.jpg|center|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]
[[File:SUT_2.jpg|center|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:22:39Z

Xddjhh: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves. [[File:SUT_1.jpg|text-bottom|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]
[[File:SUT_2.jpg|text-bottom|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:20:03Z

Xddjhh: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.[[File:SUT_1.jpg|right|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves. .[[File:SUT_2.jpg|right|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:19:13Z

Xddjhh: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

===Expression===
The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.[[File:SUT_1.jpg|right|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves. .[[File:SUT_2.jpg|right|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:16:39Z

Xddjhh: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

----
OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants

===Expression===
The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. They all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1 . This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.[[File:SUT_1.jpg|right|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves. .[[File:SUT_2.jpg|right|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:13:45Z

Xddjhh: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

===Expression===
The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. They all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1 . This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.[[File:SUT_1.jpg|right|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves. .[[File:SUT_2.jpg|right|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os10g0404500

2014-06-04T12:12:04Z

Xddjhh: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

===Expression===
The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. They all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1 . This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.[[File:SUT_1.jpg|right|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]
OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves. .[[File:SUT_2.jpg|right|thumb|150px|" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

File:SUT 2.jpg

2014-06-04T12:11:08Z

Xddjhh: Expression site

Expression site

Os10g0404500

2014-06-04T12:05:38Z

Xddjhh: /* Expression */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

===Expression===
The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. They all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1 . This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the ''Arabidopsis'' AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.[[File:SUT_1.jpg|right|thumb|150px|" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference <ref name="ref1" />).. "]]

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

File:SUT 1.jpg

2014-06-04T12:01:51Z

Xddjhh: blast of the five family

blast of the five family

Os10g0404500

2014-06-04T11:44:17Z

Xddjhh: /* Function */

Please input one-sentence summary here.

==Annotated Information==
===Function===
Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
{{JaponicaGene|
GeneName = Os10g0404500|
Description = Sucrose transporter|
Version = NM_001071090.1 GI:115481923 GeneID:4348577|
Length = 6221 bp|
Definition = Oryza sativa Japonica Group Os10g0404500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 10|Chromosome 10]]|
AP = Chromosome 10:14136198..14142418|
CDS = 14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310|
GCID = <gbrowseImage1>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008403:14136198..14142418
source=RiceChromosome10
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>|
AA = <aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>|
DNA = <dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001071090.1 RefSeq:Os10g0404500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 10]]
[[Category:Chromosome 10]]

Os05g0158500

2014-06-04T07:55:04Z

Xddjhh: /* Location */

Please input one-sentence summary here.

==Annotated Information==
This is a minor QTL for GW,also known as GS5. Forty-one SNPs and six InDels were detected in the 6.4-kb genomic region of GS5. Of these, 34 SNPs and three InDels were located in the promoter; two InDels were detected in the ﬁrst exon, resulting in a change of two to ﬁve Glys. Three contiguous SNPs were located in the second exon, leading to two amino acid changes; a nonsynonymous SNP was detected in the ninth exon, and the remaining six SNPs were located in an intron and the 3’UTR.
===Function===
GS5 is a positive regulator of grain width,ﬁlling,and weight. It encodes a putative serine carboxypeptidase,which belongs to the peptidase S10 family.It functions putatively as a positive modulator upstream of cell cycle genes, and its overexpression can result in an increase in cell numbers by promoting mitotic division. Besides,it can increase cell size to some extent leading to enhanced latitudinal growth in the grain.

===Expression===
It finds that the levels of GS5 transcript varied drastically among tissues by comparison the temporal and spatial expression pattern of GS5 in two different species, which have different size of grain, using quantitative real-time PCR (qRT-PCR) with total RNA from 14 tissues. Studies also find that the transcript was much more abundant in plants whose grains are wide than those grains are narrow in the palea/lemma at 2, 4 and 5 d before heading, and in the endosperm at 10d after fertilization. These expression differences corresponded well with the critical stages for grain width and grain filling.

===Location===
Using a double haploid (DH) population (92 lines) derived from a cross between Zhenshan 97 and H94 (both Oryza sativa L. ssp. indica), GS5 was detected in the interval between two molecular markers RM593 and RM574 on the short arm of chromosome 5.
[[File:GS5 location.jpg]]

==Labs working on this gene==
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.

==References==
[1]Lu L, Shao D, Qiu X, et al. Natural variation and artificial selection in four genes determine grain shape in rice[J]. New Phytologist, 2013, 200(4): 1269-1280.

[2]Li Y, Fan C, Xing Y, et al. Natural variation in GS5 plays an important role in regulating grain size and yield in rice[J]. Nature genetics, 2011, 43(12): 1266-1269.

==Structured Information==
{{JaponicaGene|
GeneName = Os05g0158500|
Description = Peptidase S10, serine carboxypeptidase family protein|
Version = NM_001061245.1 GI:115462220 GeneID:4337873|
Length = 4466 bp|
Definition = Oryza sativa Japonica Group Os05g0158500, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
AP = Chromosome 5:3417351..3421816|
CDS = 3417558..3417704,3417799..3417904,3418132..3418227,3418328..3418476,3418562..3418834 ,3419337..3419425,3420365..3420457,3420585..3420680,3420812..3420919 ,3421359..3421650|
GCID = <gbrowseImage1>
name=NC_008398:3417351..3421816
source=RiceChromosome05
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008398:3417351..3421816
source=RiceChromosome05
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggcggtggcggcggcggcggcggcgaggcggcgagatgtctcttgtcttctcctcctcctgtgcttcagctcgtcaatggcggcgaccggcggcggcggcggcggcggcgagcaggaggctgacagggtggcgcggctcccggggcagccggcgagccccgccgtgtcgcagttcgccggctacgtcggcgtcgacgagcggcacggcagggcgctgttctactggttcttcgaggcgcaggcgtcgccggcgccggagaagaagccgctgctcctctggctcaatggaggacctggttgctcttccattggctatggtgctgcatcagagctagggcctctcagagttgcaagacaaggagcagcactagagttcaaccagtatggctggaacaaagaggctaacctcctgttcttggagtctcctgttggggtcggcttctcctacaccaacacatcctctgacctctccaatcttaatgatgattttgtagctgaggatgcttatagtttcctggtgaactggttcaagagatttcctcagtacaaggacaatgagttctacatctcaggggagagctatgcaggtcactatgtgccccaacttgctgaccttgtctatgagaggaacaaagacaaaagggccagcacatatatcaacctcaaggggttcattgtaggcaaccctctcaccgatgattactatgattcaaaaggtttggctgagtatgcctggagccatgcaattgtgtcggatcaagtttacgagcgcatcaagaaaacttgcaacttcaagaactccaactggactgacgattgcaatgctgcaatgaacataatcttcagtcagtacaatcagatagatatatacaacatttatgccccaaagtgccttctaaacagtacttcagcatcttcacctgatcgtgcattcttcgcaaataaccaggaacaattcaggtggagaatcaagatgttttcaggctatgatccatgctactcatcctatgctgaggactacttcaataagcatgatgtgcaagaagcattccatgcaaatgccagtggactgcttccagggaagtggcaagtttgcagtgaccaaatcctcaactcttacaatttctcagtactttccatcctacctatatactccaagctcatcaaagcagggctgagagtttggctctacagcggtgatgctgacggcagggtcccggttatcagttctcggtactgcgtggaagcactcggcctaccgatcaagaccgattggcaatcttggtaccttgacaagcaggttgctgggaggtttgtggagtaccatggaatgacaatggtgactgtcagaggggcaggtcacctggttcccctcaacaaacctgctgaagggcttatgctgataaatgcattccttcatggtgagaagcttccgacaagcagatga</cdnaseq>|
AA = <aaseq>MAVAAAAAARRRDVSCLLLLLCFSSSMAATGGGGGGGEQEADRV ARLPGQPASPAVSQFAGYVGVDERHGRALFYWFFEAQASPAPEKKPLLLWLNGGPGCS SIGYGAASELGPLRVARQGAALEFNQYGWNKEANLLFLESPVGVGFSYTNTSSDLSNL NDDFVAEDAYSFLVNWFKRFPQYKDNEFYISGESYAGHYVPQLADLVYERNKDKRAST YINLKGFIVGNPLTDDYYDSKGLAEYAWSHAIVSDQVYERIKKTCNFKNSNWTDDCNA AMNIIFSQYNQIDIYNIYAPKCLLNSTSASSPDRAFFANNQEQFRWRIKMFSGYDPCY SSYAEDYFNKHDVQEAFHANASGLLPGKWQVCSDQILNSYNFSVLSILPIYSKLIKAG LRVWLYSGDADGRVPVISSRYCVEALGLPIKTDWQSWYLDKQVAGRFVEYHGMTMVTV RGAGHLVPLNKPAEGLMLINAFLHGEKLPTSR</aaseq>|
DNA = <dnaseqindica>4113..4259#3913..4018#3590..3685#3341..3489#2983..3255#2392..2480#1360..1452#1137..1232#898..1005#167..458#aagaacaccaaacgagaagctccaagctcgcaataattcaccaagaatcgcaccacttttcaccttttcttctcctcctccatttccatccatggcgacgctgctgctgctgctgcacacggcgcaacagtgagctatagctagagctcggcaccacgggttagcaatggcggtggcggcggcggcggcggcgaggcggcgagatgtctcttgtcttctcctcctcctgtgcttcagctcgtcaatggcggcgaccggcggcggcggcggcggcggcgagcaggaggctgacagggtggcgcggctcccggggcagccggcgagccccgccgtgtcgcagttcgccggctacgtcggcgtcgacgagcggcacggcagggcgctgttctactggttcttcgaggcgcaggcgtcgccggcgccggagaagaagccgctgctcctctggctcaatggaggtctcgatcgcttctgctttttttttttctaaaaaaaaagaatttgctgcagtaaaagctctgttaaatgttaatcggttaaaaattctgttagttaagtaagtaattactcctagtaaaaactacagtttctgtttgttcttttgtgtgtgcgatatgaaaaattcagatattttctcttgggtatttgttctgtggagtacttgtcgtttatgtttgtttagttttgagatgaggtttcaggttaaatttgtgatcattggttaatctgaacttctgaagagctgcttattgattttttttctttcaactgatcatattgcataaatataaataaataaatgattgttgcatgcaacatttatgttctttcttgatggttaattaggaatatttttttttctaaatggtttgttaattttgttcttggttttgtcaggacctggttgctcttccattggctatggtgctgcatcagagctagggcctctcagagttgcaagacaaggagcagcactagagttcaaccagtatggctggaacaaaggtgcagagtttattttgtttcctttaattcctgaattatttaaatatttttcatgcttaacatcactgacatttgtctttagtataattctgaaaacattttttttgtgctataaaattggtctttgccagaggctaacctcctgttcttggagtctcctgttggggtcggcttctcctacaccaacacatcctctgacctctccaatcttaatgatgattttgtaggtaattaagctgattgtaaaatgatactctagtttgcttggccatctacaggtgatctgaatttcgattgccttgtaataatgattagaagtgtaattttgtggcatcctttttttttttgttgcagctgaggatgcttatagtttcctggtgaactggttcaagagatttcctcagtacaaggacaatgagttctacatctcaggggagagctatgcaggtaaactcatttcagaaatgaaaccggtaaattttctaatcagttttgtatggtctaacattcagaattcttcattttcatcaagaaattaaaagatttcctaaccgcaacatattacaaaatctggtgtgagacatttttctcatttgttctcatgtggatgtaaacatgtccaagatgccttcctttcaagattccagttatctaatcaaaatggaaggcagcatcccaatccttgaccaaaattttcttatcgtttgctttcacaatatctcaaggattgtatgtatcgaattatgttagtgtcaccatcttatctgaaaccagaacttccactgaagaaaaaaatcatcactgactttatagaactagctgacacctatcatgagttcatgacagctcttagttcagcaatcatccccttagcaagcaaacaaaagatcatacttttaatggtgaaagtgtcagatcaggaaccaagtctctaaaataatataaggtttagtttcagccttctcaaatattcctgaagtacaatattctcagagtcatctgattgttcagaattcagaagaaatttatgtagaattcagtaaaagatgacctgcttaaacaaaaggtagaatgatttcagttgttacttgcatttatgtattgaggacagaaattataccgatgccatccgaactgatagattttctctgcttgaaacaaagtttgagtttttggattgtaatattggtccattagatctctgaacatatgtccctgaaagaatgaccaaagcatcagtggtaggttcacgggctacattgggcttctttcacaatccacatgctgtggaccagaagatgctctgcaaaaaaagcttttgttctgcacatcatgcaagatgaatctcaatctgaacttttcctgtgcaatgtccctgttatacaggtcactatgtgccccaacttgctgaccttgtctatgagaggaacaaagacaaaagggccagcacatatatcaacctcaaggggttcattgtgagaatttcttgaattgctttcagtaattattgttatggttttgccaaaaagttcttttatgtgaaatgtgctcatgtataagaaaccatatacaaacttagaaagtagaaagaactgtttactctcaccgtttcatattgcttgtcattttagctttgtcttaagtcaaacattccttacaacttacgccataaacttttactcataagatttatgtttttagattgaacaaaagaaatactttcataatatataattcacatgttcttaaactatatgaatttacagaaattgatggtcaaagttaggtatattcgacttaggacaaaactaaaacgacaagtaatttgaaacggagggagtattttgaacaatagtttctcagttgtaatgctcacaggaacacccaattggaacacgattaacttccatttaaaccatcctaaactcataaaattggttcctgcatgaacataacagtcaaaaaatctgcttgcaggtaggcaaccctctcaccgatgattactatgattcaaaaggtttggctgagtatgcctggagccatgcaattgtgtcggatcaagtttacgagcgcatcaagaaaacttgcaacttcaagaactccaactggactgacgattgcaatgctgcaatgaacataatcttcagtcagtacaatcagatagatatatacaacatttatgccccaaagtgccttctaaacagtacttcagcatcttcacctgatcgtgcattcttcgcaaataaccaggtaaaactgatccatatgcaaaaaaaaggataaagtttcattctggaccctcttctgaaattcatgcatttctgtgttttgacaggaacaattcaggtggagaatcaagatgttttcaggctatgatccatgctactcatcctatgctgaggactacttcaataagcatgatgtgcaagaagcattccatgcaaatgccagtggactgcttccagggaagtggcaagtttgcaggtgaattagcccaaagatttttcagttttatatttcaatgtattagccattaattcttttagcttgacaggaagctgagctcatccatttctttttgcagtgaccaaatcctcaactcttacaatttctcagtactttccatcctacctatatactccaagctcatcaaagcagggctgagagtttggctctacaggtttgcctcatcttaatcttagacctgttcaattaaatccatttttcagttttttttatcattcacctcagatcttccccattatcctccatactacagttcttgtacagtttctctgaaaccttgaaattactgttcaaatatattggtagtaatatgcatctacataaaaaatatgcaacaatgccctacaatttacctgaatcagtctctgttcatcaaagcagcggtgatgctgacggcagggtcccggttatcagttctcggtactgcgtggaagcactcggcctaccgatcaagaccgattggcaatcttggtaccttgacaagcaggtctgcaaaccagaagctgaactgaacatcaatacatcatgtgatttgacttgtatacatgctgaaacatggagcaaaatatttcaatttgcaggttgctgggaggtttgtggagtaccatggaatgacaatggtgactgtcagaggggcaggtcacctggttcccctcaacaaacctgctgaagggcttatgctgataaatgcattccttcatggtgagaagcttccgacaagcagatgatatgcgctgctgatgaagctttggaggtacaagatggcatgagatacaggcatggacatcaattttcattgaaaggcgccaatcggatagatttgatatggtagtatccaaatcaatttcaagtgcgccgtaaattcagtactctgttgaacattcttttaaatctgttgttgactatgactttcttcttttgtgttttttttactc</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001061245.1 RefSeq:Os05g0158500]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 5]]
[[Category:Chromosome 5]]

File:GS5 location.jpg

2014-06-04T07:53:56Z

Xddjhh: Locations of GS5 and qSW5/GW5 in the genetic map

Locations of GS5 and qSW5/GW5 in the genetic map