RiceWiki - User contributions [en]

Os08g0187500

2018-01-20T10:30:36Z

Xiaonan liu:

The rice '''''Os08g0187500''''' was reported as '''''ROC1''''' in 2016 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Function===
* ROC1 is a positive regulator of cold stress responses.

===Phenotypic analysis===
* To detect the role of ROC1 in response to chilling stress, a ROC1 T-DNA mutant and the corresponding wild-type plants were compared. In this mutant, T-DNA was located in the second intron of ROC1 gene.
* In normal growth conditions in the growth chamber, no visible phenotypes were observed between roc1 mutants and wild-type plants (Figure 4c). Next, we tested chilling stress responses in wild-type and roc1 mutants. Fifteen-day-old plants were transferred to a low temperature chamber (4 °C) for four days and moved back to the normal growth chamber (28 °C) to determine the survival rates.After 10 days of recovery, ~94% of wild-type plants were recovered, while ~16% of roc1 mutants survived.

===Expression===
* The ROC1 expression pattern was examined in roots, leaves, shoot apices and flowers tissues. ROC1 mRNA was expressed in all tissues and was found to be highly expressed in shoot apices.
* The expression levels of ROC1 in four T2 transgenic plants were analyzed by qRT-PCR. The results showed that ROC1 was suppressed at different levels and that the Ri5 plant showed strong suppression.
* To verify the phenotypic expression of roc1 mutants in response to chilling stress, the survival rate was analyzed from Ri3 and Ri5 transgenic lines.

===Subcellular localization===
* To understand the subcellular localization of ROC1, ROC1-GFP was expressed in onion epidermis cells under the control of a non-specific promoter (35S). Free GFP expression was observed in the cytosol and nucleus, while ROC1-GFP was detected in the nucleus.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Xu Y, Zhang S, Guo H, Wang S, Xu L, Li C, Qian Q, Chen F, Geisler M, Qi Y,
Jiang de A. OsABCB14 functions in auxin transport and iron homeostasis in rice
(Oryza sativa L.). Plant J. 2014 Jul;79(1):106-17. doi: 10.1111/tpj.12544. Epub
2014 Jun 13. PubMed PMID: 24798203.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 8]]

Os01g0141000

2018-01-20T10:10:04Z

Xiaonan liu:

The rice '''''Os01g0141000''''' was reported as '''''OsRAV2''''' in 2015 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Gene Symbol===
*'''''Os01g0141000''''' '''''<=>''''' '''''OsRAV2''''','''''RAV2'''''
===Function===
* our results indicate that the GT-1 element directly controls the salt response of OsRAV2. This study provides a better understanding of the putative functions of OsRAVs and the molecular regulatory mechanisms of plant genes under salt stress.

===Expression===
* In this study, the expression patterns of all fve OsRAVs were examined under salt stress. Only one gene, OsRAV2, was stably induced by high-salinity treatment.
* Further expression profle analyses indicated that OsRAV2 is transcriptionally regulated by salt, but not KCl, osmotic stress, cold or ABA (abscisic acid) treatment.
* To elucidate the regulatory mechanism of the stress response at the transcriptional level, we isolated and characterized the promoter region of OsRAV2 (POsRAV2). Transgenic analysis indicated that POsRAV2 is induced by salt stress but not osmotic stress or ABA treatment.

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==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Duan YB, Li J, Qin RY, Xu RF, Li H, Yang YC, Ma H, Li L, Wei PC, Yang JB.
Identification of a regulatory element responsible for salt induction of rice
OsRAV2 through ex situ and in situ promoter analysis. Plant Mol Biol. 2016
Jan;90(1-2):49-62. doi: 10.1007/s11103-015-0393-z. Epub 2015 Oct 19. PubMed PMID:
26482477.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]

Os03g0225700

2018-01-20T09:23:50Z

Xiaonan liu:

The rice '''''Os03g0225700''''' was reported as '''''OsWAK25''''' in 2014 <ref name="ref1" /> by researchers from USA, Belgium and China.

==Annotated Information==
===Gene Symbol===
*'''''Os03g0225700''''' '''''<=>''''' '''''OsWAK25''''','''''WAK25'''''
===Function===
* Overexpression of rice Wall-Associated Kinase 25 (OsWAK25) alters resistance to bacterial and fungal pathogens.

===Expression===
* Overexpression of OsWAK25 confers resistance to Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae.
* OsWAK25 overexpression plants display a lesion mimic phenotype and enhanced expression of NH1 and key defense genes.
* Overexpression of OsWAK25 confers enhanced susceptiblity to necrotrophic pathogens, Cochliobolus miyabeanus and Rhizoctonia solani.
* Overexpression of XB15 compromises resistance to Xoo conferred by OsWAK25 overexpression.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Harkenrider M, Sharma R, De Vleesschauwer D, Tsao L, Zhang X, Chern M, Canlas
P, Zuo S, Ronald PC. Overexpression of Rice Wall-Associated Kinase 25 (OsWAK25)
Alters Resistance to Bacterial and Fungal Pathogens. PLoS One. 2016 Jan
21;11(1):e0147310. doi: 10.1371/journal.pone.0147310. eCollection 2016. PubMed
PMID: 26795719; PubMed Central PMCID: PMC4721673.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os03g0225700

2018-01-19T10:50:25Z

Xiaonan liu:

The rice '''''Os03g0225700''''' was reported as '''''OsWAK25''''' in 2014 <ref name="ref1" /> by researchers from USA, Belgium and China.

==Annotated Information==
===Gene Symbol===
*'''''Os03g0225700''''' '''''<=>''''' '''''OsWAK25''''','''''WAK25'''''
===Function===
Please input function information here.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Harkenrider M, Sharma R, De Vleesschauwer D, Tsao L, Zhang X, Chern M, Canlas
P, Zuo S, Ronald PC. Overexpression of Rice Wall-Associated Kinase 25 (OsWAK25)
Alters Resistance to Bacterial and Fungal Pathogens. PLoS One. 2016 Jan
21;11(1):e0147310. doi: 10.1371/journal.pone.0147310. eCollection 2016. PubMed
PMID: 26795719; PubMed Central PMCID: PMC4721673.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os03g0151800

2018-01-19T05:18:06Z

Xiaonan liu:

The rice '''''Os03g0151800''''' was reported as '''''OsCDC48''''' in 2015 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Function===
* The results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.

===Phenotypic analysis===
* The premature senescence and death 128 (originally coded as HM128) mutant was obtained from mutagenesis of cultivar IR64 using ethyl methane sulfonate (EMS).
* psd128 displayed lower germination rate and slower germination (Figure 1A), retarded growth and development of roots and leaves than those of the wild-type IR64. The most significant changes between psd128 and IR64 appeared at the 6-leaf seedling stage under field conditions when the second leaf of psd128 became yellowish with brown spots.
* This phenotype first appeared on the leaf tip and spread gradually downward to cover the whole leaf blade. Subsequently, the older leaves turn white and wilted gradually, and the plants died almost completely except a few flag leaves at the early heading stage.

===Expression===
* OsCDC48 was expressed constitutively in the root, stem, leaf and panicle.
* To determine the relative abundance of the transcripts, we performed quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and found that the expression level of OsCDC48 in the roots was significantly higher than those in the stems, leaves and panicles.
* The expression levels of OsCDC48 were similar in the stems, leaves and panicles. Finally, we carried out the promoter assay in transgenic Nipponbare plants and found that GUS was detected in the roots, stems, leaves and panicles/spikelets in consistent to the qRT-PCR.

===Subcellular localization===
* Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Huang QN, Shi YF, Zhang XB, Song LX, Feng BH, Wang HM, Xu X, Li XH, Guo D, Wu
JL. Single base substitution in OsCDC48 is responsible for premature senescence
and death phenotype in rice. J Integr Plant Biol. 2016 Jan;58(1):12-28. doi:
10.1111/jipb.12372. Epub 2015 Sep 1. PubMed PMID: 26040493; PubMed Central PMCID:
PMC5049647.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os03g0151800

2018-01-19T05:15:26Z

Xiaonan liu:

Please input one-sentence summary here.

==Annotated Information==
===Function===
* The results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.

===Phenotypic analysis===
* The premature senescence and death 128 (originally coded as HM128) mutant was obtained from mutagenesis of cultivar IR64 using ethyl methane sulfonate (EMS).
* psd128 displayed lower germination rate and slower germination (Figure 1A), retarded growth and development of roots and leaves than those of the wild-type IR64. The most significant changes between psd128 and IR64 appeared at the 6-leaf seedling stage under field conditions when the second leaf of psd128 became yellowish with brown spots.
* This phenotype first appeared on the leaf tip and spread gradually downward to cover the whole leaf blade. Subsequently, the older leaves turn white and wilted gradually, and the plants died almost completely except a few flag leaves at the early heading stage.

===Expression===
* OsCDC48 was expressed constitutively in the root, stem, leaf and panicle.
* To determine the relative abundance of the transcripts, we performed quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and found that the expression level of OsCDC48 in the roots was significantly higher than those in the stems, leaves and panicles.
* The expression levels of OsCDC48 were similar in the stems, leaves and panicles. Finally, we carried out the promoter assay in transgenic Nipponbare plants and found that GUS was detected in the roots, stems, leaves and panicles/spikelets in consistent to the qRT-PCR.

===Subcellular localization===
* Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Huang QN, Shi YF, Zhang XB, Song LX, Feng BH, Wang HM, Xu X, Li XH, Guo D, Wu
JL. Single base substitution in OsCDC48 is responsible for premature senescence
and death phenotype in rice. J Integr Plant Biol. 2016 Jan;58(1):12-28. doi:
10.1111/jipb.12372. Epub 2015 Sep 1. PubMed PMID: 26040493; PubMed Central PMCID:
PMC5049647.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os03g0151800

2018-01-19T05:15:02Z

Xiaonan liu:

Please input one-sentence summary here.

==Annotated Information==
===Function===
* Our results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.

===Phenotypic analysis===
* The premature senescence and death 128 (originally coded as HM128) mutant was obtained from mutagenesis of cultivar IR64 using ethyl methane sulfonate (EMS).
* psd128 displayed lower germination rate and slower germination (Figure 1A), retarded growth and development of roots and leaves than those of the wild-type IR64. The most significant changes between psd128 and IR64 appeared at the 6-leaf seedling stage under field conditions when the second leaf of psd128 became yellowish with brown spots.
* This phenotype first appeared on the leaf tip and spread gradually downward to cover the whole leaf blade. Subsequently, the older leaves turn white and wilted gradually, and the plants died almost completely except a few flag leaves at the early heading stage.

===Expression===
* OsCDC48 was expressed constitutively in the root, stem, leaf and panicle.
* To determine the relative abundance of the transcripts, we performed quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and found that the expression level of OsCDC48 in the roots was significantly higher than those in the stems, leaves and panicles.
* The expression levels of OsCDC48 were similar in the stems, leaves and panicles. Finally, we carried out the promoter assay in transgenic Nipponbare plants and found that GUS was detected in the roots, stems, leaves and panicles/spikelets in consistent to the qRT-PCR.

===Subcellular localization===
* Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Huang QN, Shi YF, Zhang XB, Song LX, Feng BH, Wang HM, Xu X, Li XH, Guo D, Wu
JL. Single base substitution in OsCDC48 is responsible for premature senescence
and death phenotype in rice. J Integr Plant Biol. 2016 Jan;58(1):12-28. doi:
10.1111/jipb.12372. Epub 2015 Sep 1. PubMed PMID: 26040493; PubMed Central PMCID:
PMC5049647.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os04g0607600

2018-01-19T03:12:27Z

Xiaonan liu:

The rice '''''Os04g0607600''''' was reported as '''''HKT1;4''''' in 2016 <ref name="ref1" /> by researchers from Japan, Italy and USA.

==Annotated Information==
===Gene Symbol===
*'''''Os04g0459000''''' '''''<=>''''' '''''OsHKT7''''','''''HKT7'''''
===Function===
* OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress.

===Expression===
* Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19).
* OsHKT1;4 expression increases Na+ hypersensitivity of yeast cells promoting Na+ over-accumulation. The OsHKT1;4 cDNA from rice cultivar Nipponbare was constitutively expressed in strain G19 of S. cerevisiae under the control of the GAP promoter.
* G19 cells harboring the empty vector or expressing OsHKT1;4 were grown on the arginine phosphate (AP) medium containing 1 mM KCl with or without the addition of 50 mM NaCl.

===Phenotypic analysis===
* phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress.

===Subcellular localization===
* To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Suzuki K, Yamaji N, Costa A, Okuma E, Kobayashi NI, Kashiwagi T, Katsuhara M,
Wang C, Tanoi K, Murata Y, Schroeder JI, Ma JF, Horie T. OsHKT1;4-mediated Na(+)
transport in stems contributes to Na(+) exclusion from leaf blades of rice at the
reproductive growth stage upon salt stress. BMC Plant Biol. 2016 Jan 19;16:22.
doi: 10.1186/s12870-016-0709-4. PubMed PMID: 26786707; PubMed Central PMCID:
PMC4719677.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]]

Os04g0607600

2018-01-19T03:11:19Z

Xiaonan liu:

The rice '''''Os04g0607600''''' was reported as '''''HKT1;4''''' in 2016 <ref name="ref1" /> by researchers from Japan, Italy and USA.

==Annotated Information==
===Gene Symbol===
*'''''Os04g0459000''''' '''''<=>''''' '''''OsHKT7''''','''''HKT7'''''
===Function===
* OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress.
===Expression===
* Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19).
* OsHKT1;4 expression increases Na+ hypersensitivity of yeast cells promoting Na+ over-accumulation. The OsHKT1;4 cDNA from rice cultivar Nipponbare was constitutively expressed in strain G19 of S. cerevisiae under the control of the GAP promoter.
* G19 cells harboring the empty vector or expressing OsHKT1;4 were grown on the arginine phosphate (AP) medium containing 1 mM KCl with or without the addition of 50 mM NaCl.

===Phenotypic analysis===
* phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress.
===Evolution===
* To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Suzuki K, Yamaji N, Costa A, Okuma E, Kobayashi NI, Kashiwagi T, Katsuhara M,
Wang C, Tanoi K, Murata Y, Schroeder JI, Ma JF, Horie T. OsHKT1;4-mediated Na(+)
transport in stems contributes to Na(+) exclusion from leaf blades of rice at the
reproductive growth stage upon salt stress. BMC Plant Biol. 2016 Jan 19;16:22.
doi: 10.1186/s12870-016-0709-4. PubMed PMID: 26786707; PubMed Central PMCID:
PMC4719677.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]]

Os04g0607600

2018-01-18T13:58:44Z

Xiaonan liu:

The rice '''''Os04g0607600''''' was reported as '''''HKT1;4''''' in 2016 <ref name="ref1" /> by researchers from Japan, Italy and USA.

==Annotated Information==
===Gene Symbol===
*'''''Os04g0459000''''' '''''<=>''''' '''''OsHKT7''''','''''HKT7'''''
===Function===
* OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress.
===Expression===
* Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19).

===Phenotypic analysis===
* phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress.
===Evolution===
* To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Suzuki K, Yamaji N, Costa A, Okuma E, Kobayashi NI, Kashiwagi T, Katsuhara M,
Wang C, Tanoi K, Murata Y, Schroeder JI, Ma JF, Horie T. OsHKT1;4-mediated Na(+)
transport in stems contributes to Na(+) exclusion from leaf blades of rice at the
reproductive growth stage upon salt stress. BMC Plant Biol. 2016 Jan 19;16:22.
doi: 10.1186/s12870-016-0709-4. PubMed PMID: 26786707; PubMed Central PMCID:
PMC4719677.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]]

Os03g0191400

2018-01-18T13:26:57Z

Xiaonan liu:

The rice '''''Os03g0191400''''' was reported as '''''OsRab6a''''' in 2016 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Gene Symbol===
*'''''Os04g0459000''''' '''''<=>''''' '''''OsRab6B2''''','''''OsRab6a'''''
===Function===
* OsRab6a plays an important role in the regulation of Fe acquisition in rice plants by modulating Downloaded from http://pcp.oxfordjournals.org/ at Loyola University of Chicago on April 25, 20164 physiological processes involved in Fe acquisition and root system architecture in response to Fe-deficient medium.

===Expression===
* The expression of OsRab6a was rapidly and transiently up-regulated by Fe deficiency.
* No differences in growth and development among the OsRab6a-overexpression, OsRab6a-RNAi and wild-type plants were detected when grown in Fe-sufficient medium.
* However, overexpression of OsRab6a conferred rice plants greater tolerance to Fe deficiency than RNAi and wild-type plants, as evidenced by higher seedling height, greater biomass, chlorophyll contents and Fe concentrations in shoots, roots and grains in the overexpression lines.
* Moreover, the overexpression lines exhibited greater root systems than wild-type and RNAi plants in Fe-deficient medium.

===Subcellular localization===
* The OsRab6a-GFP fusion gene and GFP gene were introduced into tobacco leaf cells by Agrobacterium-mediated transient transformation method, respectively. Both the OsRab6a-GFP fusion protein and GFP alone were expressed throughout the whole cell.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Yang A, Zhang WH. A Small GTPase, OsRab6a, is Involved in the Regulation of
Iron Homeostasis in Rice. Plant Cell Physiol. 2016 Jun;57(6):1271-80. doi:
10.1093/pcp/pcw073. Epub 2016 Apr 11. PubMed PMID: 27257291.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os06g0693700

2018-01-18T13:06:52Z

Xiaonan liu:

The rice '''''Os06g0693700''''' was reported as '''''OsSIDP366''''' in 2015 <ref name="ref1" /> by researchers from China and USA.

==Annotated Information==
===Function===
* OsSIDP366 may function as a regulator of the PBs/SGs and positively regulate salt and drought resistance in rice.

===Expression===
* Transgenic rice plants overexpressing OsSIDP366 showed enhanced drought and salinity tolerance and reduced water loss as compared to that in the control, whereas plants with downregulated OsSIDP366 expression levels using RNA interference (RNAi) were more sensitive to salinity and drought treatments.
* The sensitivity to abscisic acid (ABA) treatment was not changed in OsSIDP366-overexpressing plants, and OsSIDP366 expression was not affected in ABAdeficient mutants.
* Digital gene expression (DGE) profile analysis indicated that stress-related genes such as SNAC1, OsHAK5 and PRs were upregulated in OsSIDP366-overexpressing plants.

===Subcellular localization===
* Subcellular localization analysis revealed that OsSIDP366 is presented in the cytoplasmic foci that colocalized with protein markers for both processing bodies (PBs) and stress granules (SGs) in rice protoplasts.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Guo C, Luo C, Guo L, Li M, Guo X, Zhang Y, Wang L, Chen L. OsSIDP366, a
DUF1644 gene, positively regulates responses to drought and salt stresses in
rice. J Integr Plant Biol. 2016 May;58(5):492-502. doi: 10.1111/jipb.12376. Epub
2015 Sep 25. PubMed PMID: 26172270.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 6]]

Os05g0276100

2018-01-18T10:39:22Z

Xiaonan liu:

The rice '''''Os05g0276100''''' was reported as '''''OsCHX14''''' in 2014 <ref name="ref1" /> by researchers from Netherlands, UK and China.

==Annotated Information==
===Gene Symbol===
*'''''Os04g0459000''''' '''''<=>''''' '''''OsCHX14'''''
===Function===
* OsCHX14 may play an important role in K+ homeostasis during flowering in rice.

===Expression===
* Expression profiling confirmed that among 17 CHX genes in rice, OsCHX14 was the only member that showed an expression polymorphism, not only in osjar1 mutants but also in RNAi (RNA interference) lines of OsCOI1, another key member of the JA signaling pathway. This suggests that the expression of OsCHX14 is regulated by the JA signaling pathway.
* Promoter–b-glucuronidase (GUS) analysis of transgenic rice revealed that OsCHX14 is mainly expressed in lodicules and the region close by throughout the flowering process.

===Subcellular localization===
* OsCHX14 is localized to the endoplasmic reticulum. The OsCHX14 ORF was cloned in-frame with a GFP reporter gene and transiently expressed in Arabidopsis root protoplasts together with an ER marker tagged with an RFP reporter.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Chen Y, Ma J, Miller AJ, Luo B, Wang M, Zhu Z, Ouwerkerk PB. OsCHX14 is
Involved in the K+ Homeostasis in Rice (Oryza sativa) Flowers. Plant Cell
Physiol. 2016 Jul;57(7):1530-1543. Epub 2016 May 6. PubMed PMID: 27903806.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]]

Os04g0271700

2018-01-18T10:17:15Z

Xiaonan liu:

The rice '''''Os04g0271700''''' was reported as '''''OscZOG1''''' in 2015 <ref name="ref1" /> by researchers from China.
==Annotated Information==
===Function===
* Putative zeatin O-glucosyltransferase OscZOG1 regulates root and shoot development and formation of agronomic traits in rice

===Expression===
* We found that OscZOG1 was preferentially expressed in shoot and root meristematic tissues and nascent organs.
* The growth of lateral roots was stimulated in the overexpression lines, but inhibited in RNA interference lines.
* In shoots, knockdown of OscZOG1 expression by RNA interference significantly improved tillering, panicle branching, grain number per panicle and seed size, which are important agronomic traits for grain yield.

===Subcellular localization===
* The resulting fragment was introduced into 35S-GFP-JFH1 vector with the restriction sites XhoI and SpeI to generate a Cterminal GFP fusion construct. The OscZOG1-GFP constructs were transferred into Agrobacterium tumefaciens strain GV3101. Subcellular localization analysis showed that OscZOG1 was found to be localized both in cytoplasm and nucleus.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Shang XL, Xie RR, Tian H, Wang QL, Guo FQ. Putative zeatin
O-glucosyltransferase OscZOG1 regulates root and shoot development and formation
of agronomic traits in rice. J Integr Plant Biol. 2016 Jul;58(7):627-41. doi:
10.1111/jipb.12444. Epub 2016 Jan 2. PubMed PMID: 26507364.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]]

Os03g0308200

2018-01-18T09:25:20Z

Xiaonan liu:

The rice '''''Os03g0308200''''' was reported as '''''nrl2''''' in 2016 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Gene Symbol===
*'''''Os04g0459000''''' '''''<=>''''' '''''NRL2''''','''''SRL2'''''
===Function===
* NRL2 regulates leaf and flower development in rice.

===Phenotypic analysis===
* To isolate the novel gene that regulates rice leaf development, the researchers screened two indica line mutant populations that were generated by ethyl methanesulphonate (EMS) mutagenesis and identified two allelic mutants with extremely narrow and semi-rolled leaves, named nrl2-1 and nrl2-2.
* Phenotypic observation of leaf growth showed that the nrl2-1 mutant had a significantly reduced leaf blade width, attaining approximately 55.6% of the leaf width of the wild type (WT) at 70 days after sowing.
* They also found that the average number of both large veins and small veins between two adjacent large veins in the mature leaves of nrl2-1 was significantly reduced compared with WT.

===Expression===
* We analyzed the expression profile of NRL2 in rice using quantitative RT-PCR (qRT-PCR), which showed that the NRL2 gene displayed constitutive expression in all rice tissues and organs examined, with maximum expression in young leaves.
* We then analyzed the expression pattern of the NRL2 gene in different leaves from seedlings with three unfolded leaves. This showed that the NRL2 gene was expressed in all of the leaves examined, with maximum expression in the shoot apex region (SAR) and a gradual reduction from the SAR to the first leaf (L1).
* To determine the spatial and temporal expression patterns of NRL2 during leaf development more precisely, we performed mRNA in situ hybridization, which showed that NRL2 was particularly highly expressed in the leaf primordia, mesophyll cells, and sclerenchymatous cells.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Zhao S, Zhao L, Liu F, Wu Y, Zhu Z, Sun C, Tan L. NARROW AND ROLLED LEAF 2
regulates leaf shape, male fertility, and seed size in rice. J Integr Plant Biol.
2016 Dec;58(12):983-996. doi: 10.1111/jipb.12503. Epub 2016 Nov 23. PubMed PMID:
27762074.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os03g0308200

2018-01-18T09:20:04Z

Xiaonan liu:

Os03g0198600

2018-01-18T04:34:24Z

Xiaonan liu:

The rice '''''Os03g0198600''''' was reported as '''''HOX12''''' in 2016 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Gene Symbol===
*'''''Os03g0198600''''' '''''<=>''''' '''''OsHox12''''','''''Hox12'''''
===Function===
* Rice HOX12 regulates panicle exsertion by directly modulating the expression of ELONGATED UPPERMOST INTERNODE1 (EUI1).

===Expression===
* Diminished HOX12 expression by RNA interference enhanced panicle exsertion, mimicking the eui1 phenotype. HOX12 knockdown plants contain higher levels of the major biologically active GAs (such as GA1 and GA4) than the wild type.
* The expression of EUI1 is elevated in the ree1-D mutant but reduced in HOX12 knockdown plants. Interestingly, both HOX12 and EUI1 are predominantly expressed in panicles, where GA4 is highly accumulated.

===Subcellular localization===
* The researchers further investigated the subcellular localization of HOX12 by expressing HOX12-enhanced GFP (eGFP) fusion proteins under the control of the CaMV 35S promoter in rice protoplasts. Empty eGFP vector was transfected into rice protoplasts as a control. As expected, eGFP itself was distributed evenly in the cytoplasm and the nucleus, whereas theHOX12-eGFP fusion protein was preferentially localized to the nucleus.
* Yeast one-hybrid, electrophoretic mobility shift assay, and chromatin immunoprecipitation analyses showed that HOX12 physically interacts with the EUI1 promoter both in vitro and in vivo. Furthermore, plants overexpressing HOX12 in the eui1 mutant background retained the elongated uppermost internode phenotype.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Gao S, Fang J, Xu F, Wang W, Chu C. Rice HOX12 Regulates Panicle Exsertion by
Directly Modulating the Expression of ELONGATED UPPERMOST INTERNODE1. Plant Cell.
2016 Mar;28(3):680-95. doi: 10.1105/tpc.15.01021. Epub 2016 Mar 14. PubMed PMID:
26977084; PubMed Central PMCID: PMC4826014.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os03g0198600

2018-01-18T04:09:43Z

Xiaonan liu:

Os02g0701300

2018-01-18T03:57:42Z

Xiaonan liu:

The rice '''''Os02g0701300''''' was reported as '''''OsGRF4''''' in 2016 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Gene Symbol===
*'''''Os02g0701300''''' '''''<=>''''' '''''OsGRF4''''','''''GRF4'''''
===Function===
* OsGRF4 controls grain shape, panicle length and seed shattering in rice.

===Phenotypic analysis===
* A unique OsGRF4 mutation, which occurs at the OsmiRNA396 target site of OsGRF4, seems to be associated with high levels of OsGRF4 expression, and results in phenotypic difference.
* Further research showed that OsGRF4 regulated two cytokinin dehydrogenase precursor genes (CKX5 and CKX1) resulting in increased cytokinin levels, which might affect the panicle traits.

===Expression===
* We compared the expression profiles of OsGRF4 in various organs of NILs by quantitative RT-PCR analysis with total RNA. The OsGRF4 transcript levels varied drastically among the tissues.
* OsGRF4 was preferentially expressed in developing panicles, and the highest levels of expression were found in panicles of 7 cm in length.
* there was less transcript accumulation in the rice hull, root, stem and leaf sheath. In particular, the transcript was much more abundant in NIL-OsGRF4 than in NIL-Osgrf4 in the young panicles measuring 1 cm, 4 cm, 7 cm, 11 cm and 15 cm in length.
* The differences corresponded with the critical stages of panicle traits. The OsGRF4 effect on panicle traits might be attributed to differences in expression levels.

===Subcellular localization===
* To determine the sub-cellular localization of OsGRF4, the coding sequence of OsGRF4 was fused with yellow fluorescent protein (YFP). In contrast, Ghd7, which is a nuclear protein, was fused with cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. The constructs were co-transfected into rice protoplasts of etiolated seedlings by polyethylene glycol. The result showed that OsGRF4 co-localized to the nucleus with Ghd7.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Sun P, Zhang W, Wang Y, He Q, Shu F, Liu H, Wang J, Wang J, Yuan L, Deng H.
OsGRF4 controls grain shape, panicle length and seed shattering in rice. J Integr
Plant Biol. 2016 Oct;58(10):836-847. doi: 10.1111/jipb.12473. Epub 2016 Jun 9.
PubMed PMID: 26936408; PubMed Central PMCID: PMC5089622.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]

Os02g0701300

2018-01-18T03:54:32Z

Xiaonan liu:

The rice '''''Os02g0701300''''' was reported as '''''OsGRF4''''' in 2016 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Gene Symbol===
*'''''Os02g0701300''''' '''''<=>''''' '''''OsGRF4''''','''''GRF4'''''
===Function===
* OsGRF4 controls grain shape, panicle length and seed shattering in rice.

===Phenotypic analysis===
* A unique OsGRF4 mutation, which occurs at the OsmiRNA396 target site of OsGRF4, seems to be associated with high levels of OsGRF4 expression, and results in phenotypic difference.
* Further research showed that OsGRF4 regulated two cytokinin dehydrogenase precursor genes (CKX5 and CKX1) resulting in increased cytokinin levels, which might affect the panicle traits.

===Expression===
* We compared the expression profiles of OsGRF4 in various organs of NILs by quantitative RT-PCR analysis with total RNA. The OsGRF4 transcript levels varied drastically among the tissues.
* OsGRF4 was preferentially expressed in developing panicles, and the highest levels of expression were found in panicles of 7 cm in length.

===Subcellular localization===
* To determine the sub-cellular localization of OsGRF4, the coding sequence of OsGRF4 was fused with yellow fluorescent protein (YFP). In contrast, Ghd7, which is a nuclear protein, was fused with cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. The constructs were co-transfected into rice protoplasts of etiolated seedlings by polyethylene glycol. The result showed that OsGRF4 co-localized to the nucleus with Ghd7.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
==References==
<references>
* <ref name="ref1">
Sun P, Zhang W, Wang Y, He Q, Shu F, Liu H, Wang J, Wang J, Yuan L, Deng H.
OsGRF4 controls grain shape, panicle length and seed shattering in rice. J Integr
Plant Biol. 2016 Oct;58(10):836-847. doi: 10.1111/jipb.12473. Epub 2016 Jun 9.
PubMed PMID: 26936408; PubMed Central PMCID: PMC5089622.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]

Os08g0220600

2018-01-18T03:15:13Z

Xiaonan liu:

The rice '''''Os08g0220600''''' was reported as '''''OsVIL1''''' in 2016 <ref name="ref1" /> by researchers from South Korea.

==Annotated Information==
===Function===
* OsVIL1 controls flowering time in rice by suppressing OsLF under short days and by inducing Ghd7 under long days.

===Phenotypic analysis===
* The reduction in OsVIL1 expression by RNA interference (RNAi) caused a late flowering phenotype under short days (SD).
* OsVIL1-overexpressing (OX) transgenic lines displayed an early flowering phenotype under SD.

===Expression===
* The researchers investigated the role of Oryza sativa VERNALIZATION INSENSITIVE 3-LIKE 1 (OsVIL1), which is homologous to the flowering promoter OsVIL2.
* The reduction in OsVIL1 expression by RNA interference (RNAi) caused a late flowering phenotype under short days (SD).
* In the RNAi lines, OsLF expression was increased, but transcripts of Early heading date 1 (Ehd1), Heading date 3a (Hd3a), and RICE FLOWERING LOCUS T 1 (RFT1) were reduced.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Jeong HJ, Yang J, Cho LH, An G. OsVIL1 controls flowering time in rice by
suppressing OsLF under short days and by inducing Ghd7 under long days. Plant
Cell Rep. 2016 Apr;35(4):905-20. doi: 10.1007/s00299-015-1931-5. Epub 2016 Jan
21. PubMed PMID: 26795142.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 8]]

Os08g0220600

2018-01-18T03:13:45Z

Xiaonan liu:

The rice '''''Os08g0220600''''' was reported as '''''OsVIL1''''' in 2016 <ref name="ref1" /> by researchers from South Korea.

==Annotated Information==
===Function===
* OsVIL1 controls flowering time in rice by suppressing OsLF under short days and by inducing Ghd7 under long days.

===Phenotypic analysis===
* The reduction in OsVIL1 expression by RNA interference (RNAi) caused a late flowering phenotype under short days (SD).
* OsVIL1-overexpressing (OX) transgenic lines displayed an early flowering phenotype under SD.

===Expression===
* We investigated the role of Oryza sativa VERNALIZATION INSENSITIVE 3-LIKE 1 (OsVIL1), which is homologous to the flowering promoter OsVIL2.
* The reduction in OsVIL1 expression by RNA interference (RNAi) caused a late flowering phenotype under short days (SD).
* In the RNAi lines, OsLF expression was increased, but transcripts of Early heading date 1 (Ehd1), Heading date 3a (Hd3a), and RICE FLOWERING LOCUS T 1 (RFT1) were reduced.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Jeong HJ, Yang J, Cho LH, An G. OsVIL1 controls flowering time in rice by
suppressing OsLF under short days and by inducing Ghd7 under long days. Plant
Cell Rep. 2016 Apr;35(4):905-20. doi: 10.1007/s00299-015-1931-5. Epub 2016 Jan
21. PubMed PMID: 26795142.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 8]]

Os03g0237200

2018-01-17T14:47:35Z

Xiaonan liu:

The rice '''''Os03g0237200''''' was reported as '''''LPA1''''' in 2015 <ref name="ref1" /> by researchers from Korea and Japan.

==Annotated Information==
===Function===
* Loose Plant Architecture1 (LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo brassinosteroids in rice.

===Phenotypic analysis===
* The researchers examined the mode of action of LPA1 on lamina bending by comparing the phenotypes among knockout, overexpression and repressor-fusion transgenic plants.
* A strong synergic effect is detected between lpa1 and d2 (the defective mutant for catalysis of C-23-hydroxylated BRs) during IAA-mediated lamina inclination.

===Expression===
* The Ubiquitin promoter was used to overexpress LPA1 cDNA. To construct a vector expressing repressor-fused LPA1, the C-terminus of LPA1 was fused with the 24 amino acid fragment containing a conserved ERF-associated amphiphilic repression (EAR) motif of a gene (LOC_Os02g01090) orthologous to Arabidopsis SUPERMAN (At3g23130).
* RNA sequencing analysis and qRT-PCR indicate that LPA1 inﬂuences the expression of three OsPIN genes (OsPIN1a, OsPIN1c and OsPIN3a), which suggests that auxin ﬂux might be an important factor in LPA1-mediated lamina inclination in rice.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Liu JM, Park SJ, Huang J, Lee EJ, Xuan YH, Je BI, Kumar V, Priatama RA, Raj K
V, Kim SH, Min MK, Cho JH, Kim TH, Chandran AK, Jung KH, Takatsuto S, Fujioka S,
Han CD. Loose Plant Architecture1 (LPA1) determines lamina joint bending by
suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo
brassinosteroids in rice. J Exp Bot. 2016 Mar;67(6):1883-95. doi:
10.1093/jxb/erw002. Epub 2016 Jan 29. PubMed PMID: 26826218; PubMed Central
PMCID: PMC4783368.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

Os03g0237200

2018-01-17T14:44:02Z

Xiaonan liu:

Os02g0491600

2018-01-17T13:57:47Z

Xiaonan liu:

The rice '''''Os02g0491600''''' was reported as '''''OsGLP2-1''''' in 2016 <ref name="ref1" /> by researchers from China.

==Annotated Information==
===Function===
* The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice.
* OsGLP2-1 functions as a positive regulator to modulate disease resistance. Its good quantitative resistance to the two major diseases in rice makes it to be a promising target in rice breeding.

===Expression===
* Overexpression of OsGLP2-1 quantitatively enhanced resistance to leaf blast, panicle blast and bacterial blight.
* Compared with empty vector transformed (control) plants, the OsGLP2-1 overexpressing plants exhibited higher levels of H2O2 both before and after pathogen inoculation.
* Overexpression of OsGLP2-1 induced three well-characterized defense-related genes which are associated in JA-dependent pathway after pathogen infection.

===Subcellular localization===
* The temporal and spatial expression analysis revealed that OsGLP2-1is highly expressed in leaves and panicles and sub-localized in the cell wall.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Liu Q, Yang J, Yan S, Zhang S, Zhao J, Wang W, Yang T, Wang X, Mao X, Dong J,
Zhu X, Liu B. The germin-like protein OsGLP2-1 enhances resistance to fungal
blast and bacterial blight in rice. Plant Mol Biol. 2016 Nov;92(4-5):411-423.
Epub 2016 Sep 15. PubMed PMID: 27631432.
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]

Os02g0491600

2018-01-17T13:43:58Z

Xiaonan liu:

Os05g0187100

2018-01-16T13:40:46Z

Xiaonan liu:

The rice '''''Os05g0187100''''' was reported as '''''OsHXK7''''' in 2015 <ref name="ref1" /> by researchers from Korea.

==Annotated Information==
===Gene Symbol===
*'''''Os05g0187100''''' '''<=>''' '''''OsHXK7''''','''''HXK7'''''

===Function===
* OsHXK7 plays in sugar metabolism and efficient germination by enforcing glycolysis-mediated fermentation in O2-deficient rice.

===Phenotypic analysis===
* The germination of two null OsHXK7 mutants, oshxk7-1 and oshxk7-2, was affected by O2 deficiency, but overexpression enhanced germination in rice.

===Expression===
* According to transient expression assays that used the promoter:luciferase fusion construct, OsHXK7 enhanced the glucose (Glc)-dependent repression of a rice a-amylase gene (RAmy3D) in the mesophyll protoplasts of maize.
* the expression of OsHXK7, but not its catalytically inactive alleles, complemented the Arabidopsis glucose insensitive2-1 (gin2-1) mutant, thereby resulting in the wild type characteristics of Glc-dependent repression,seedling development, and plant growth.
* OsHXK7-mediated Glc-dependent repression was abolished in the O2-deficient mesophyll protoplasts of maize.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Kim HB, Cho JI, Ryoo N, Shin DH, Park YI, Hwang YS, Lee SK, An G, Jeon JS.
Role of rice cytosolic hexokinase OsHXK7 in sugar signaling and metabolism. J
Integr Plant Biol. 2016 Feb;58(2):127-35. doi: 10.1111/jipb.12366. Epub 2015 Aug
24. PubMed PMID: 25951042.

</ref>
</references>
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]]

Os05g0187100

2018-01-16T13:40:06Z

Xiaonan liu:

The rice '''''Os05g0187100''''' was reported as '''''OsHXK7''''' in 2015 <ref name="ref1" /> by researchers from Korea.

==Annotated Information==
===Gene Symbol===
*'''''Os05g0187100''''' '''<=>''' '''''OsHXK7''''','''''HXK7'''''

===Function===
* OsHXK7 plays in sugar metabolism and efficient germination by enforcing glycolysis-mediated fermentation in O2-deficient rice.

===Phenotypic analysis===
* The germination of two null OsHXK7 mutants, oshxk7-1 and oshxk7-2, was affected by O2 deficiency, but overexpression enhanced germination in rice.

===Expression===
* According to transient expression assays that used the promoter:luciferase fusion construct, OsHXK7 enhanced the glucose (Glc)-dependent repression of a rice a-amylase gene (RAmy3D) in the mesophyll protoplasts of maize.
* the expression of OsHXK7, but not its catalytically inactive alleles, complemented the Arabidopsis glucose insensitive2-1 (gin2-1) mutant, thereby resulting in the wild type characteristics of Glc-dependent repression,seedling development, and plant growth.
* OsHXK7-mediated Glc-dependent repression was abolished in the O2-deficient mesophyll protoplasts of maize.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
* Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
* State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

==References==
<references>
* <ref name="ref1">
Kim HB, Cho JI, Ryoo N, Shin DH, Park YI, Hwang YS, Lee SK, An G, Jeon JS.
Role of rice cytosolic hexokinase OsHXK7 in sugar signaling and metabolism. J
Integr Plant Biol. 2016 Feb;58(2):127-35. doi: 10.1111/jipb.12366. Epub 2015 Aug
24. PubMed PMID: 25951042.

</ref>
</references>
</ref>
</references>

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]]

Os05g0187100

2018-01-16T13:33:00Z

Xiaonan liu:

Os05g0187100

2018-01-16T13:29:19Z

Xiaonan liu:

The rice '''''Os05g0187100''''' was reported as '''''OsHXK7''''' in 2014 <ref name="ref1" /> by researchers from Korea.

==Annotated Information==
===Function===
Please input function information here.

===Expression===
Please input expression information here.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
Please input cited references here.

==Structured Information==
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]]