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Os11g0446000

2014-06-08T02:46:02Z

Xiaowugui9: /* Evolution */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|60px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:45:24Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|60px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:40:57Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|60px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:40:42Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|100px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

File:RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg

2014-06-08T02:39:43Z

Xiaowugui9: uploaded a new version of "File:RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg": (A) bHLH094 enhances the activity of the gene promoter containing E-box (CANNTG). (B) A combinational expression of RSS3 and JAZ9 suppres

File:RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg

2014-06-08T02:38:14Z

Xiaowugui9:

Os11g0446000

2014-06-08T02:35:38Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|150px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:34:04Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File: RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|200px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:29:41Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg|right|thumb|100px|" RSS3, bHLH, and JAZ Form a Ternary Complex(from reference <ref name="ref1" />. "]]
[[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|100px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:28:23Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg and Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|100px|" RSS3, bHLH, and JAZ Form a Ternary Complex(from reference <ref name="ref1" />. " and "RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:26:46Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg|right|thumb|100px|" RSS3, bHLH, and JAZ Form a Ternary Complex(from reference <ref name="ref1" />."]][[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right File|thumb|100px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:26:00Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg|right|thumb|100px|" RSS3, bHLH, and JAZ Form a Ternary Complex(from reference <ref name="ref1" />."]][[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right right|thumb|100px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:25:32Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg|right|thumb|100px|" RSS3, bHLH, and JAZ Form a Ternary Complex(from reference <ref name="ref1" />."]]
[[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right right|thumb|100px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:25:00Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg|right|thumb|200px|" RSS3, bHLH, and JAZ Form a Ternary Complex(from reference <ref name="ref1" />."]]
[[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|150px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:19:59Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
[[File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg|right|thumb|150px|" RSS3, bHLH, and JAZ Form a Ternary Complex(from reference <ref name="ref1" />."]]
[[File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg|right|thumb|150px|"RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation(from reference <ref name="ref1" />."]]
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg

2014-06-08T02:17:58Z

Xiaowugui9: (A) bHLH094 enhances the activity of the gene promoter containing E-box (CANNTG). (B) A combinational expression of RSS3 and JAZ9 suppresses the bHLH094-mediated gene activation driven by the promoter containing G-box (CACGTG). Effector plasmids containi

(A) bHLH094 enhances the activity of the gene promoter containing E-box (CANNTG).

(B) A combinational expression of RSS3 and JAZ9 suppresses the bHLH094-mediated gene activation driven by the promoter containing G-box (CACGTG). Effector plasmids containing bHLH094, RSS3, RSS3_D21, and JAZ9 driven by the CaMV 35S promoter were introduced into rice protoplasts, concomitant with a reporter plasmid containing a triplicate E-box (A) or G-box (B) fused to the basal promoter-fLUC (reporter). fLUC activity was measured and normalized with that of rLUC. Empty vector was added to equalize the amount of plasmids transfected in each combination. Mean ± sd; n = 3 to 8. Asterisks indicate a Student’s t test P value < 0.05 in (A). Statistical differences were determined by one-way ANOVA followed by Tukey posthoc analysis, and different letters indicate significant differences (P value < 0.05) in (B). fLUC, firefly luciferase; rLUC, renilla luciferase.

File:Figure1. RSS3, bHLH, and JAZ Form a Ternary Complex.jpg

2014-06-08T02:17:10Z

Xiaowugui9: (A) Yeast three-hybrid assay to verify interaction of RSS3N318, bHLH094, and JAZ9. Representative results of more than six independent transformants are shown for each combination. D21 represents the 21–amino acid deletion mimicking rss3 mutation. The c

(A) Yeast three-hybrid assay to verify interaction of RSS3N318, bHLH094, and JAZ9. Representative results of more than six independent transformants are shown for each combination. D21 represents the 21–amino acid deletion mimicking rss3 mutation. The control and selective medium were as described in Figure 5. Media lacking Met (-M) was used for induction of the gene encoding the 3rd protein by MET25 promoter for testing interaction between the three proteins expressed. “Empty” means the vector control. 3AT, 3-amino-1,2,4-triazole

(B) and (C) Interaction among JAZ9, RSS3N203, and bHLH094, as analyzed by BiFC-apFRET. Representative images (B) and FRET efficiencies (C) of apFRET in onion cells using YFPN-RSS3N203, bHLH094-YFPc, and ECFP-JAZ9 or nonfused ECFP are shown. Pseudocolor images represent fluorescence intensities. Bar = 10 µm. Mean ± sd; n = 3 (ECFP) and 6 (ECFP-JAZ9). Asterisks indicate P value < 0.05 (Student’s t test).

Os11g0446000

2014-06-08T02:11:46Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:11:35Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref1" />]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:10:34Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1","ref2" />."]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:10:06Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" ,ref name="ref2" />."]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:09:46Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" /,ref name="ref2" />."]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:09:17Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1" />,<ref name="ref2" />."]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:08:43Z

Xiaowugui9: /* References */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1，2" />."]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.

2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:08:27Z

Xiaowugui9: /* References */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1，2" />."]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation. The Plant Cell, 2013, 25(5): 1709-1725.
2. Godoy M., Franco-Zorrilla J.M., Pérez-Pérez J., Oliveros J.C., Lorenzo Ó., Solano R.Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors. Plant J.2011, 66: 700–711.

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T02:05:35Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
[[File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg|right|thumb|150px|"RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins(from reference <ref name="ref1，2" />."]]
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

File:RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domains of RB-Like bHLH Proteins.jpg

2014-06-08T02:03:59Z

Xiaowugui9: (A) Map-based cloning of RSS3 (top) and the schematic representation of the RSS3 gene structure (bottom). The rss3 locus was delimited between the indicated markers. The chromosomal position of the markers and the number of F2 lines exhibiting the rss3 ph

(A) Map-based cloning of RSS3 (top) and the schematic representation of the RSS3 gene structure (bottom). The rss3 locus was delimited between the indicated markers. The chromosomal position of the markers and the number of F2 lines exhibiting the rss3 phenotype among 1501 lines examined are provided in brackets and parentheses, respectively. Exons (closed boxes), 5′- or 3′-untranslated regions (open boxes), and the position of the first codon (ATG), stop codon (TAG), and 47-bp deletion in rss3 are indicated.

(B) Schematic structure of RSS3 and an R/B-like bHLH protein, MYC2, of Arabidopsis. The regions conserved between RSS3 and At-MYC2 (I to IV) and the position of the 21–amino acid (a.a.) deletion in rss3 are shown. bHLH represents the basic helix-loop-helix DNA binding domain.

(C) The amino acid sequences of the N-terminal region of RSS3, RSS3-related proteins, and R/B-like bHLH factors were aligned using the MAFFT program. The red dotted line represents the sequence lacking in rss3. The conserved regions (I to IV) and JAZ interacting domain of At-MYC2 and At-MYC3 (Fernández-Calvo et al., 2011) are indicated.

(D) GFP signals of rice roots carrying RSS3-EGFP driven by the RSS3 promoter or EGFP driven by the Actin promoter. The red arrowhead indicates the border between the MZ and EZ. Magnified views of the MZ (top) and EZ (bottom) are shown in the right panels. Bars = 200 μm (left) and 10 μm (right panels).

Os11g0446000

2014-06-08T01:59:44Z

Xiaowugui9: /* Mutant */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-08T01:55:05Z

Xiaowugui9: /* Mutant */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.
[[File:Impaired Root Growth of rss3 under Salinity Conditions.jpg|right|thumb|150px|"Impaired Root Growth of rss3 under Salinity Conditions(from reference <ref name="ref1" />."]]

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

File:Impaired Root Growth of rss3 under Salinity Conditions.jpg

2014-06-08T01:46:21Z

Xiaowugui9: uploaded a new version of "File:Impaired Root Growth of rss3 under Salinity Conditions.jpg"

(A) and (B) Wild-type (WT) and rss3 seedlings were grown with (+) or without (−) 100 mM NaCl for 7 d.

(A) Seedlings.

(B) Roots floating on water.

(C) Propidium iodide staining of the primary roots of 3-d-old wild-type and rss3 seedlings, grown in the presence or absence of 100 mM NaCl. Red and white arrowheads indicate the border of the MZ and EZ, and of the EZ and MTZ, respectively. Images were generated by concatenating two or more pictures.

(D) Length (left) and number of cells (right) in the MZ and EZ of the root tips of 3-d-old wild-type and rss3 seedlings, grown with or without 100 mM NaCl. Mean ± sd, n ≥ 3. Asterisks indicate a Student’s t test P value < 0.05.

(E) The MTZs of 7-d-old roots of wild-type and rss3 seedlings grown in the presence or absence of NaCl were observed by differential interference contrast microscopy.

Bars = 5 cm in (A), 1 cm in (B), 400 µm in (C), and 100 µm in (E).

Os11g0446000

2014-06-07T14:01:56Z

Xiaowugui9: /* Labs working on this gene */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

*Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

*National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

*Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T14:01:17Z

Xiaowugui9: /* Labs working on this gene */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==

Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T14:00:54Z

Xiaowugui9: /* Labs working on this gene */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:34:43Z

Xiaowugui9: /* Labs working on this gene */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan
National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:31:48Z

Xiaowugui9: /* Labs working on this gene */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan
National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:30:03Z

Xiaowugui9: /* Labs working on this gene */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:28:32Z

Xiaowugui9: /* Function */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.
RSS3 and bHLH094 can interact in plant cells.Among more than 150 bHLH factors in rice, bHLH094 belongs to the class-C group. The distinguishing feature of class-C members is the conservation of bHLH sequences and the subsequent C-terminal regions.RSS3 can specifically interact with bHLH094 and bHLH089,but not with bHLH092.bHLH089 is classified in the same minor clade that bHLH094 belongs to.Interestingly, both full-length bHLH094 and bHLH089 lack the N-terminal regulatory domains that are conserved among R/B-like bHLH factors and RSS3. The genes encoding bHLH094 and bHLH089 were expressed in the root tip, so RSS3 acts as a regulatory module for bHLH094 and bHLH089 and thus is directly involved in transcriptional regulation in the root tip.
As described above, RSS3, but not bHLH094 or bHLH089, contains the regulatory domain conserved among R/B-like bHLH transcription factors. Considering that region II of the regulatory domain is a part of the JAZ interacting domain, so RSS3 is capable of binding to JAZ proteins.
RSS3 forms a ternary complex with rice bHLH094 and JAZ9,and RSS3 and JAZ together repress bHLH094-mediated transcriptional activation.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:14:58Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.
In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:10:47Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm<ref name="ref1" />.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:10:17Z

Xiaowugui9: /* Expression */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 Encodes a Nuclear Protein Homologous to the Regulatory Domain of the R/B-Like bHLH Transcription Factors.RSS3 encodes a protein consisting of 458 amino acids. RSS3 contains a region homologous to the postulated regulatory domain conserved among the R/B-like bHLH transcription factors, which contains four conserved regions (I to IV). Region II is predicted to constitute the central sequence of the JAZ interacting domain. However, RSS3 does not contain a bHLH domain or another known DNA binding motif. rss3 contains a 47-bp deletion at the junction between the 6th intron and 7th exon , which results in an altered splicing pattern and the production of a mutated protein that lacks 15 amino acids in region IV and six amino acids in the subsequent C-terminal nonconserved region.In seedlings, RSS3 was expressed predominantly in the roots, particularly in the root tip. A truncated form of RSS3 transcript was expressed in the mutant roots, at levels that were apparently higher than those of the nondeleted RSS3 transcript in the wild type. When enhanced green fluorescent protein (EGFP)–tagged RSS3 was expressed under the control of the RSS3 promoter, EGFP signals were detected at high levels in the MZ and at low levels in the EZ of the root tip. The fusion protein was localized almost exclusively in the nuclei of the MZ cells. By contrast, nonfused EGFP expressed under the control of the constitutive actin gene promoter was localized in both nuclei and the cytoplasm of MZ cells. In the EZ region bordering the MZ, RSS3-EGFP was detected in both the nucleus and the cytoplasm.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:02:20Z

Xiaowugui9: /* Mutant */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 is preferentially expressed in the root tip and forms a ternary complex with class-C basic helix-loop-helix (bHLH) transcription factors and JASMONATE ZIM-DOMAIN proteins, the latter of which are the key regulators of jasmonate (JA) signaling. The mutated protein arising from the rss3 allele fails to interact with bHLH factors, and the expression of a significant portion of JA-responsive genes is upregulated in rss3.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T13:01:44Z

Xiaowugui9: /* Mutant */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.
[[File:Impaired Root Growth of rss3 under Salinity Conditions.(from reference <ref name="ref1" /> )]]

===Expression===
RSS3 is preferentially expressed in the root tip and forms a ternary complex with class-C basic helix-loop-helix (bHLH) transcription factors and JASMONATE ZIM-DOMAIN proteins, the latter of which are the key regulators of jasmonate (JA) signaling. The mutated protein arising from the rss3 allele fails to interact with bHLH factors, and the expression of a significant portion of JA-responsive genes is upregulated in rss3.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

File:Impaired Root Growth of rss3 under Salinity Conditions.jpg

2014-06-07T12:59:20Z

Xiaowugui9: uploaded a new version of "File:Impaired Root Growth of rss3 under Salinity Conditions.jpg"

File:Impaired Root Growth of rss3 under Salinity Conditions.jpg

2014-06-07T12:57:56Z

Xiaowugui9: (A) and (B) Wild-type (WT) and rss3 seedlings were grown with (+) or without (−) 100 mM NaCl for 7 d. (A) Seedlings. (B) Roots floating on water. (C) Propidium iodide staining of the primary roots of 3-d-old wild-type and rss3 seedlings, grown in the

Os11g0446000

2014-06-07T12:51:28Z

Xiaowugui9: /* Mutant */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness<ref name="ref1" />.

===Expression===
RSS3 is preferentially expressed in the root tip and forms a ternary complex with class-C basic helix-loop-helix (bHLH) transcription factors and JASMONATE ZIM-DOMAIN proteins, the latter of which are the key regulators of jasmonate (JA) signaling. The mutated protein arising from the rss3 allele fails to interact with bHLH factors, and the expression of a significant portion of JA-responsive genes is upregulated in rss3.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T12:49:00Z

Xiaowugui9: /* Mutant */

RSS3(rice salt sensitive 3) is a rice nuclear factor,that regulates root cell elongation during adaptation to salinity.

==Annotated Information==
===Function===
RSS3(rice salt sensitive 3), has a regulatory role in root cell elongation. RSS3 interacts not only with JAZs, but also with non-R/B-like bHLH transcription factors and forms an RSS3-JAZ-bHLH ternary complex in the nucleus. Loss of function of RSS3 activates the expression of a subset of JA-induced genes in the root apex and restricts cell elongation but does not primarily affect cell division activity. Under high-salinity conditions, rss3 mutants exhibit severely inhibited root growth, concomitant with root cell swelling<ref name="ref1" />.Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions.

===Mutant===
In the course of a genetic screen of rice to identify loci responsible for salt tolerance, we identified a recessive mutant designated rss3. When grown under high-salinity conditions, rss3 exhibited severely impaired root growth compared with the wild type. Root growth in the absence of salinity stress was also inhibited in rss3, but only moderately. The length of cells in the MTZ of roots of rss3 plants grown under unstressed conditions was markedly shorter than those of the wild type. The inhibition of root growth in rss3 appeared to be primarily due to reduced cell elongation because the size of the EZ, but not of the MZ, was reduced in the mutant, and the number of cells in both the EZ and MZ was not affected in the mutant. The exaggerated inhibition of root growth under salinity conditions also appeared to be mainly due to compromised cell elongation but was accompanied by aberrant cellular arrangement and the formation of oblique cell plates in the MZ, swelling of the cells in the EZ, and a waviness of the root surface at the MTZ. Moreover, rss3 roots show impaired flexibility, in a salinity-dependent manner. When extracted from the medium, the wild-type roots exhibited a paintbrush-like shape, but rss3 roots did not, due to stiffness.

===Expression===
RSS3 is preferentially expressed in the root tip and forms a ternary complex with class-C basic helix-loop-helix (bHLH) transcription factors and JASMONATE ZIM-DOMAIN proteins, the latter of which are the key regulators of jasmonate (JA) signaling. The mutated protein arising from the rss3 allele fails to interact with bHLH factors, and the expression of a significant portion of JA-responsive genes is upregulated in rss3.

===Evolution===
Please input evolution information here.

You can also add sub-section(s) at will.

==Labs working on this gene==
Please input related labs here.

==References==
1. Yosuke Toda;Maiko Tanaka;Daisuke Ogawa;Kyo Kurata;Ken-ichi Kurotani;Yoshiki Habu;Tsuyu Ando;Kazuhiko Sugimoto;Nobutaka Mitsuda;Etsuko Katoh;Kiyomi Abe;Akio Miyao;Hirohiko Hirochika;Tsukaho Hattori;Shin Takeda
RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation
The Plant Cell, 2013, 25(5): 1709-1725

==Structured Information==
{{JaponicaGene|
GeneName = Os11g0446000|
Description = Conserved hypothetical protein|
Version = NM_001074358.2 GI:297611767 GeneID:4350435|
Length = 4813 bp|
Definition = Oryza sativa Japonica Group Os11g0446000, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 11|Chromosome 11]]|
AP = Chromosome 11:16608816..16613628|
CDS = 16611100..16611258,16612560..16613384|
GCID = <gbrowseImage1>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008404:16608816..16613628
source=RiceChromosome11
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtgataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtag</cdnaseq>|
AA = <aaseq>MPYVPSPALSSFLFLQSPLAPLLLGGRDLITATTTHLINHLQKQ AQHTATATVIPEDLHFVLRMRHMFESLGYQSGFFLSQLFSSSRGTSPSPSSFPLKQQQ PPPPQLFNWPGHAPPQLPPGASPLFPPGPAAFHPSSRPMPPFPGGGKDESHLFHLPPA AAAKQPQHMDEHHHHQQQPMAAPQQHGGEAPEGDLKWPNGLSFFTALTGRTEDAKFLF GGGGGGGADDGSKTAAAAQDAGHGGAENVEEYLSLESHSNKARRMESAQSTKFKRSFT LPARMSSSTTSTSPSVSASTAPAPPQQQQGMEYRGPHEGGVYSDLMETFLE</aaseq>|
DNA = <dnaseqindica>2285..2443#3745..4569#aagcagcagcagcaggaggtgagagagtgaagagagagacacagccaaagctcagctcagctaagctaagctctactcctactagtagtccctcttcaccctcactctacctcaccctcccccgagaaaaccccaagaaacgatcaaaaagagaccgaccagcaaggccgtagcctcaaagagcagccaaaaccacggagtttttaccatctatcttagctctagcttagctacctagctagcgccggagcagtgaaagagagagtgagagaagaagaagaggaggtggaaagaagtgaaagaaatggtgggctccggggcggcagggggagggggaggtggaggaggaggaggggatcacgcgcgcagcaaagaggccgccgggatgatggctctccacgaggccctccgcaacgtctgcctcaactccgactggacttactccgtcttctggaccatccgtcctcgcccgtatgtgtaccacttaattaattaccacccctcctatgaatttttgcctcaaactctcatctcatctcttctccctatatatatgtcgtcgaaggcggtgccgcggcggcaacgggtgcaaggtcggcgacgacaacggcagcctgtaagtgagcaattgagcatatatacgcacgcacccccaaccaatcgatcgatcgtgtgtgtagttatcaatctggggatatatcatatatgtatgcaggatgctgatgtgggaggacgggttctgccggccgcgggtggcggagtgcctggaggacatcgacggcgaggaccccgtccgcaaggccttcagcaagatgtccatccagctctacaactacggagaagggtgggtgctgggtaggtgatgcagtgcgtaatcgtgtacagtttcgagtgttttttgcccccttggttctcgtggagtcagtcgacgactcgatttggtagtcatgtcagcctctctatcgcatcgcagctgcggatttcttcgctcgaccgtgtcacccgccctgttcttggattaggacccctgcatgcgcgattaatgctaatagtttccccatgattcctgcgtcgtcgtcgtcgccgtcttagagcaagtttaatagtagagttcactactttctattagtcaatattaaagctaacatgtatagtaggttgagtataaggttatctttattttcttcgtcctctctctttctctcactatgaatttaatgcatattttttagagtttgtatacagttagcccttgcatgagaaccaacactccccacttttatttatctctctcttccacataagcatataacttgcttatagactactgttatcctcgctcttagtgtgtgaaagtgcaactctattttggcccaaccagtagtactacactagtacgaccagtcaccagagagaggaggaaaggggaggagatcgatgggatgggcgtgtgcttttttttcatctttttcgctttaaatttgttccaatatttatctaccgttccttgctcgcctgctttgcttttgggtttaaatcagtgtgtgtggtttgaattagttaacctagggtttggttgattttcggatttggtttggttttgacgacgccggggcatcacatcacgcgtggtgcagcgggggtcattatgcgtgggcagaggcgtgggtaaactacaaattaacagccacttaccccctcctaagtcagtggctttaggcgagggctgtcacgtgcgcacgttcgttggggtgcgtgggggcggtgcctcgtatcgcataaacaaatcccggcgcgtcagtggcttaattgcgggctttgtctgcggattaacaacgcccctctgctacgcgcacaacaaaactctacacagcagcaggagcagcagaattgtaaaaaaaatgtttcaagcagatggaaatgatagaatttttttttctgtaccttttcttgtgattctgcgtccatctgtacgtctatgtgagcgtgtcgatgactttagcacagtaatatagtaatatctctgtttggctactgtattggggattaatttgggtaattactactggtaattgcgttaatctggtcgctttcactttgttctactactactaccgagcttttgagcgtgatgttggtaagacgatgattttgatctggaccgctggtgggtgctgctgcaggctgatgggaaaggtggcgtctgataaatgtcacaaatgggtcttcaaggaaccttctgaatgcgagcccaacatcgccaactactggcagagctcttttgatgccgtatgtaccatctcctgcactctcctcctttctctttctgcagagccccctggcccccttgcttttggggggaagagaccttattactgctactactacacacctcattaatcacttgcaaaaacaagctcagcacactgccacagcaacagtggtaggtctcatgtttttactgcgggaatgaggcaaaaaaaagaggaaaaaacaggtaaacagtaaaagagcccgagagagatatatcgacatgtctctggggagttctcaggctttgcatcaaatctgctcgtctgctctgctcccccaaaaagaagagaggaattggatgctctctctcactctctcatcaggatatcaccaagctagtatagaattaacgccgccgatcacagtcacggctaagctttattcctgtgagaacttaaccaaattcccctctcatgcttttgcttttgcagcttcctcccgaatggactgatcagttcgcctctggcatccaggcatgcacttctgttcacctagatcttttacatgctcgtcttagtttgtgattgatatttattagtgatgctaaacttttgcttaatttgcttgtagaccatagcagtgatccaagctggccatggccttcttcagcttggctcttgcaagatcgtgagtacagctatatattgctccctctatttttttaatatataacattgttaattttattctattatatcttttaaacttccatcatcaatagttctctgaatgttcagttaaaaaacgtaaattatactccatccgttctaaaataaactaacatcatactataatgtgatgcatcctattacaacgaatcgaatctgggatgcgttatatcttagtatgaccttggttttttttaagacggatggtgtactacataggtagattttacattataaatggctgtgcaacattacaattttattggatattactccctccgttccataatataatataaggcacaactactttttttagatgttacataatataaggcatgcatacaattaatttctcttctccattaaactattatttttttaaattctccactctcgagatctctaattctattggatgcatgtattatatttattagggtgatctaaactaggagatgataataattgtttcttggtctttggattaagagtggttatacattatattttggaacagagggagtatctttataggggcaattgttatatatatatattttttgatacgaaaataacggttaaagttactccacgcaacagaccgaataaaatgtgtcatatgcatttagtaccgagaggtacagtgagacatggccctttcttgacttgtttttgtcgcgttggtcaaagacgccgacgtggacgtgtttgtggggggcactagctaattgggcaaaacgtacgtacgctaactgtgcagataccggaggacctgcacttcgtgctgcggatgcggcacatgttcgagtcgctcggctaccagtcgggcttcttcctctcccagctcttctcctcctcgcgggggacctcgccgtcgccgtcgtcgttcccgcttaagcagcagcagccgccgccgccgcagctcttcaactggccgggccacgcgccgccgcagctcccgcccggcgcgtcgccgctcttcccgcccgggccggccgcgttccacccgtcgtcccggccaatgccgcccttccccggcggcggcaaggatgagagccacctgttccatctcccgccggcggcggcggccaagcagccgcagcacatggacgagcaccaccaccaccagcagcagccgatggcggcgccgcagcagcacggcggggaggcgcccgagggcgacctgaaatggcccaacgggctgtcgttcttcaccgcgctcaccggccggaccgaggacgcgaagttcctcttcggcggcggcggaggcggcggcgcggacgacgggagcaagacggcggcggcggcgcaggacgcggggcacggcggcgcggagaacgtggaggagtacctgagcctggagagccactccaacaaggcgaggaggatggagagcgcccagagcaccaagttcaagaggagcttcacgttgccggcgaggatgagctcgtccaccacgtcgacgtccccgtcggtgtcggcgtcgacggcgccggcgccgccgcagcagcagcaagggatggagtacaggggcccacacgagggcggcgtctactcggacctaatggagacgttcttggagtagcctaagctagcttagctagcgaccacaccaagctagcaaacatggaagcatgatcatgatcaagtgtttgttcttgtcacctcttagttttttccttcttttctttctcccaaactatgtttaagcttttgagcggtctggtttggagtttgcttctgtttaattaattaattaattagatcatgtagtaatcctttttgttcctccctagttgtactagctctctccaagtgcaaaaacatga</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001074358.2 RefSeq:Os11g0446000]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 11]]
[[Category:Chromosome 11]]

Os11g0446000

2014-06-07T12:45:25Z

Xiaowugui9: /* Annotated Information */

Os11g0446000

2014-06-07T12:44:40Z

Xiaowugui9: /* mutant */

Os11g0446000

2014-06-07T12:40:20Z

Xiaowugui9: /* Annotated Information */

Os11g0446000

2014-06-07T12:35:45Z

Xiaowugui9: /* Function */