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Os06g0194900

2014-06-01T07:56:59Z

Yangmei li: /* Evolution */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6% identity.The set two genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.the evolutionary sequences of the three genes as following:
[[File:Three gene phylogenetic tree.png]]

the ancestral gene for genes 1 and 3 is given number 3 because it is considered to have 15 introns like gene 2, in other words, the 14 intron structure of gene1 is derived from a 15 intron structure by gene contraction. the isozymes so far isolated from the rice show little differences in their catalytic properties. the SS genes evolved in the plant kingdom at a very early stage. probably immediately following the evolution of sucrose-oriented photosynthetic carbon assimilation system.

===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

==Knowledge Extention==
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su,
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T07:53:38Z

Yangmei li:

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6% identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.the evolutionary sequences of the three genes as following:
[[File:Three gene phylogenetic tree.png]]

the ancestral gene for genes 1 and 3 is given number 3 because it is considered to have 15 introns like gene 2, in other words, the 14 intron structure of gene1 is derived from a 15 intron structure by gene contraction. the isozymes so far isolated from the rice show little differences in their catalytic properties. the SS genes evolved in the plant kingdom at a very early stage. probably immediately following the evolution of sucrose-oriented photosynthetic carbon assimilation system.

===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

==Knowledge Extention==
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su,
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T03:19:53Z

Yangmei li: /* Evolution */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6% identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.the evolutionary sequences of the three genes as following:
[[File:Three gene phylogenetic tree.png]]

the ancestral gene for genes 1 and 3 is given number 3 because it is considered to have 15 introns like gene 2, in other words, the 14 intron structure of gene1 is derived from a 15 intron structure by gene contraction. the isozymes so far isolated from the rice show little differences in their catalytic properties. the SS genes evolved in the plant kingdom at a very early stage. probably immediately following the evolution of sucrose-oriented photosynthetic carbon assimilation system.

===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su,
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T03:19:23Z

Yangmei li: /* Evolution */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6% identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.the evolutionary sequences of the three genes as following:
[[File:Three gene phylogenetic tree.png]]
the ancestral gene for genes 1 and 3 is given number 3 because it is considered to have 15 introns like gene 2, in other words, the 14 intron structure of gene1 is derived from a 15 intron structure by gene contraction. the isozymes so far isolated from the rice show little differences in their catalytic properties. the SS genes evolved in the plant kingdom at a very early stage. probably immediately following the evolution of sucrose-oriented photosynthetic carbon assimilation system.

===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su,
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T03:07:21Z

Yangmei li: /* Evolution */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6% identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.
[[File:Three gene phylogenetic tree.png]]

===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su,
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

File:Three gene phylogenetic tree.png

2014-06-01T03:03:56Z

Yangmei li: evolutionary sequences of the three genes

evolutionary sequences of the three genes

Os06g0194900

2014-06-01T02:48:35Z

Yangmei li: /* Evolution */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6% identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.

===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su,
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T02:46:37Z

Yangmei li: /* References */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.
===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su,
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T02:28:56Z

Yangmei li: /* Comparison between SUS1 & SUS2 */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.
===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.<ref name="ref5" />

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T02:28:07Z

Yangmei li: /* Comparison between SUS1 & SUS2 */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.
===Comparison between ''SUS1'' & ''SUS2''===
''RSus1'',is upregulated by sugars. However,''RSus2'', the Sh1-type gene, is sugar as well as starvation upregulated. Possession by the same gene of a similar response to the two contrasting carbohydrate levels is unusual, this is the first case among Sus genes.Sucrose-modulated expression of ''RSus1'' was regulated at the transcriptional level as revealed from that the transcription activity of RSus1 significantly increased in the presence of sucrose , but the stability of the transcribed ''RSus1'' mRNA was not affected by sucrose abundance . However,
there are other mechanisms involved in regulating RSus1 expression. the RSuS1 proteins are extremely stable in sucrose-starved cells. the gene products of RSus1 and RSus2 formed homo- and hetero-tetrameric RSuS isoforms in etiolated rice seedlings. RSuS1 might be stabilized through the formation of heterotetramers with RSuS2 since the RSus2 gene was expressed under sucrose starvation conditions, and the RSuS2 protein level was even higher in sucrose-starved cells than in sucrose-fed cells. Regulation of RSus2 was different from that of RSus1. First, the stability of RSus2 mRNA was higher in sucrose-starved cells, suggesting that post-transcriptional regulation of RSus2 occurred. Second, although the transcriptional activity of RSus2 in sucrose-fed and sucrose-starved cells was similar, the transcription and/or the stability of RSus2 mRNA, was negatively affected by CHX. newly synthesized proteins induced by sucrose or by sucrose starvation were involved in the regulation of RSus2 expression at transcriptional and/or post-transcriptional levels. Furthermore, the changes in mRNA level and those in protein level did not show marked differences, except that the level of RSuS2 protein was higher under prolonged sucrose starvation conditions. However, it may attribute to the higher stability of the RSus2 mRNA in sucrose-starved cells.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T02:15:57Z

Yangmei li:

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that ''RSus2'' is closely related to maize Sus as is ''RSUSl'' to ''Sh1''.The amino acid sequences deduced from ''RSUS2'' and ''RSUS3'' share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of ''RSUSl''.
===Comparison between ''SUS1'' & ''SUS2''===

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T02:07:42Z

Yangmei li: /* Knowledge Extention */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.<ref name="ref5" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T02:06:48Z

Yangmei li: /* Knowledge Extention */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.
* In maize, the genes encoding for SuS, ''Sus1'' and ''Sh1'',show contrasting carbohydrate responses; that is,''Sus1'' is upregulated while ''Sh1'' is downregulated in the presence of abundant carbohydrate supplies.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-06-01T01:49:06Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of ''RSus2'' requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />
*The transcription rates of ''RSus2'' were similar in cells grown in the sucrose-free and sucrose-containing media, suggesting that there was constitutive expression of ''RSus2'' mRNA in cells under vary-ing sugar availability.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T09:12:28Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of RSus2 requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological function in rice cells other than as a housekeeping gene.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T09:11:57Z

Yangmei li: /* References */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of RSus2 requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological
function in rice cells other than as a housekeeping gene.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T09:11:32Z

Yangmei li: /* References */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of RSus2 requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological
function in rice cells other than as a housekeeping gene.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
<ref name="ref5">
</ref>Y.C Liao, A.Y Wang Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice.Physiol Plant, 118 (2003), pp. 319–327
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T09:03:28Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />
* the expression of RSus2 requires newly synthesized proteins induced by sucrose and/or starvation. RSus2 may have an important biological
function in rice cells other than as a housekeeping gene.<ref name="ref5" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T08:59:30Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress,the expression of Sus genes is also differentially affected by sugars.
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T08:56:21Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]
Expression of different Sus genes is spatially and temporally regulated and is differentially modulated in response to anoxia, low
temperatures and osmotic stress
''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T08:43:11Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role in providing sucrose to the sink tissues in general.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T08:36:09Z

Yangmei li: /* Evolution */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic function according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
Sequence similarities in SUS proteins from rice and maize indicate that RSus2 is closely related to maize Sus as is RSUSl to Sh1.The amino acid sequences deduced from RSUS2 and RSUS3 share an 89.6%identity.The set wo genes maybe evolved from the same ancestor after the divergence of RSUSl.

===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-24T08:19:45Z

Yangmei li: /* Function */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6), It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as cellulose<ref name="ref3"/>

===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-22T01:47:41Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>
===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is ubiquitously expressed,may play a house keeping role.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-22T01:47:20Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>
===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll, RSuS2 was evenly distributed in seed tissues other than the endosperm, RSus2 is
ubiquitously expressed,may play a house keeping role.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T14:43:46Z

Yangmei li: /* Knowledge Extention */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>
===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
===Knowledge Extention===
* sucrose synthase (SS) genes in Populus expression patterns were constitutive and partially overlapping but diverse.

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T09:38:46Z

Yangmei li:

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>
===Mutation===

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />
===Knowledge Extention===

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T09:23:52Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* ''SUS2'' was in the phloem in addition to the mesophyll.<ref name="ref4" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T09:23:14Z

Yangmei li: /* References */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* SUS2 was in the phloem in addition to the mesophyll.<ref name="ref4" />
*

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
<ref name="ref4">A.Y. Wang, M.H. Kao, W.H. Yang, Y. Sayion, L.F. Liu, P.D. Lee, J.C. Su, Differentially and developmentally regulated expression of three rice
sucrose synthase genes, Plant Cell Physiol. 40 (1999) 800–807.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T09:21:46Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

* SUS2 was in the phloem in addition to the mesophyll.<ref name="ref4" />
*

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T09:12:28Z

Yangmei li: /* Labs working on this gene */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan
* Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 106, R.O.C
* Department of Agronomy, National Taiwan University, Taipei, Taiwan 106, R.O.C.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:53:24Z

Yangmei li: /* Expression */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).

* ''SUS2'' transcript levels were higher in root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS transcripts.

* ''SUS2'' was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1.

* ''SUS2'' was abundantly expressed in the early stage of the grain filling at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

* ''SUS2'' was highly expressed in the developing caryopsis, correlating strongly with the stage of rapid increase in grain dry weight,and for a longer period thanSUS3, which implies that SUS2,along with SUS3 and SUS4, plays an important role in the grain filling process.

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:39:32Z

Yangmei li: /* Labs working on this gene */

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
* National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:35:08Z

Yangmei li:

The rice '''''Os06g0194900''''' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:34:27Z

Yangmei li:

The rice '''Os06g0194900'''''Italic text'' is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:33:30Z

Yangmei li:

The rice Os06g0194900 is the second gene of ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:18:19Z

Yangmei li:

The rice ''Sus'' gene family is comprised of six genes,''SUS''2 is belongs to the ''Sus'' gene family,and play a significantly role on ''Sus'' gene family.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:13:57Z

Yangmei li:

The rice Sus gene family is comprised of six genes

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:11:56Z

Yangmei li: /* Function */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose<ref name="ref3"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:11:31Z

Yangmei li: /* References */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239.</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543.</ref>
<ref name="ref3">C.H. Haigler, M. Ivanova-Datcheva, P.S. Hogan, V.V. Salnikov, S. Hwang, K. Martin, D.P. Delmer, Carbon partitioning to cellulose synthesis, Plant
Mol. Biol. 47 (2001) 29–51.</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:09:41Z

Yangmei li: /* Function */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

Recently a growing body of evidence suggests that one of the major roles of Sus is to channel UDP-glucose to the synthesis of cell-wall polysaccharides such as
cellulose

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:03:23Z

Yangmei li: /* Function */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family.The six SUS genes can be classified into three recognized Sus groups, the Sus1 group (SUS1, 2and3),the SusA group (SUS4) and the NG (SUS5 and 6),

It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T08:00:55Z

Yangmei li: /* Function */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family. It has been reported that sugar metabolism of rice seedlings is affected by hypoxia typically caused by submergence, and that Sus activity is suggested to be one of the key factors for the altered sugar metabolism in rice plants under submerged condition. overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T07:58:07Z

Yangmei li: /* Expression */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family. In addition, overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .

The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.<ref name="ref2" />

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T07:56:22Z

Yangmei li: /* Expression */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family. In addition, overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .
The mRNA levels for the SUS genes were highest in elongating sink organs such as roots, developing leaf blades and elongating uppermost internodes, with SUS1 and 2 being the isoforms predominantly expressed within these tissues. In contrast to this, SUS transcript levels in the mature leaf blades and leaf sheaths were low. The transcript levels of each SUS gene were plotted individually to examine specific expression patterns (Figure 1).''SUS''2 transcript levels were higher in
root and internode tissue than other tissues examined.In all of the internode segments the transcripts of SUS1 and SUS2 together accounted for most of the SUS
transcripts, and the transcript levels of the other four SUS genes were either negligible or not detected. SUS2 was most highly expressed in the bottom segment and decreased in subsequent segments before a slight increase in the top two segments.Overall SUS2 expression levels were considerably lower than those for SUS1. SUS1 mRNA was most abundantly expressed in the early stage of the grain filling, at 1–3 DAF, and gradually decreased thereafter. The transcript level of SUS2 was at similar levels to those of SUS1 at 1 and 2 DAF, then increased and reached maximal levels during 5–10 DAF, and decreased thereafter.

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T07:32:19Z

Yangmei li: /* Annotated Information */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family. In addition, overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .
Although all three genes are expressed in the developing kernels, ''Sh1'' has the dominant role in cell-wall synthesis whereas Sus1 plays a role in starch synthesis.
the entire Sus gene family in rice does in fact comprise the six reported genes.
the established sequences have 8950 and 7586 bp for ''RSus2'' and ''RSus3'',respectively

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T07:31:28Z

Yangmei li: /* Annotated Information */

Please input one-sentence summary here.

The rice Sus gene family is comprised of six genes
==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family. In addition, overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .
Although all three genes are expressed in the developing kernels, ''Sh1'' has the dominant role in cell-wall synthesis whereas Sus1 plays a role in starch synthesis.
the entire Sus gene family in rice does in fact comprise the six reported genes.
the established sequences have 8950 and 7586 bp for ''RSus2'' and ''RSus3'',respectively

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T07:30:31Z

Yangmei li: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family. In addition, overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .
Although all three genes are expressed in the developing kernels, ''Sh1'' has the dominant role in cell-wall synthesis whereas Sus1 plays a role in starch synthesis.
the entire Sus gene family in rice does in fact comprise the six reported genes.
the established sequences have 8950 and 7586 bp for ''RSus2'' and ''RSus3'',respectively

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-20T07:30:04Z

Yangmei li: /* Annotated Information */

Please input one-sentence summary here.

==Annotated Information==
The rice Sus gene family is comprised of six genes
===Function===
In rice three genes encoding for sucrose synthase (Sus),six genes comprise the entire rice ''Sus'' gene family. In addition, overall ''Sus'' activity and expression of ''SUS''2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress.Sucrose is cleaved ''in vivo'' either by sucrose synthase (Sus) (UDP-glucose: D-fructose 2-glucosyl-transferase, EC 2.4.1.13) or by invertase. Sus catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. There is considerable evidence to indicate that Sus plays a major role(s) in metabolism in a number of different growth processes within a variety of sink tissues.Sus is also proposed to supply UDP-glucose for cellulose synthesis in the cell wall, and in cotton fiber which is supported by experimental evidence from antisense suppression of the enzyme.<ref name="ref1"/>

===Expression===
[[File:1-s2.0-S0168945208000472-gr2.jpg|right|thumb|500px|'''Figure1.''' ''The transcript levels of the ''SUS'' genes in various rice tissues(from reference <ref name="ref2"/>).''.jpg]]

''Sus'' transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. ''SUS2'' was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s)<ref name="ref2" />

''Sus'' gene expression has also been reported to be induced in response to environmental stresses such as hypoxia and cold. ''Sus'' is reported to be encoded by multiple genes which have distinct and partially overlapping expression patterns. For example, maize has at least three genes encoding ''Sus'', ''Sh1'', ''Sus1'' and ''Sus3'' .
Although all three genes are expressed in the developing kernels, ''Sh1'' has the dominant role in cell-wall synthesis whereas Sus1 plays a role in starch synthesis.
the entire Sus gene family in rice does in fact comprise the six reported genes.
the established sequences have 8950 and 7586 bp for ''RSus2'' and ''RSus3'',respectively

===Evolution===
the evolution of multiple SS genes is not for the need of diversified catalytic functions but to meet the differential temporal and spatial requirements of the catalytic functiong according to the scheme of plant development and in response to environmental changes.this trend may be especially enhanced in the rice where the plant must adapt to the aerobic( leaf,stem,and grain) and anaerobic (root) conditions simultaneously and accumulate starch in a short period of the life span.<ref name="ref1" />

==Labs working on this gene==
Please input related labs here.

==References==
<references>
<ref name="ref1"> Ju-Wei HUANG;Jen-Tao CHEN;Wei-Ping YU;Lie-Fen SHYUR;Ai-Yu WANG;Hsien-Yi SUNG;Ping-Du LEE;Jong-Ching Su
Complete Structures of Three Rice Sucrose Synthase Isogenes and Differential Regulation of Their Expressions
Bioscience, Biotechnology, and Biochemistry, 1996, 60(2): 233-239</ref>
<ref name="ref2"> Tatsuro Hirose;Graham N. Scofield;Tomio Terao
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Science, 2008, 174(5): 534-543</ref>
</references>

==Structured Information==
{{JaponicaGene|
GeneName = Os06g0194900|
Description = Sucrose synthase 2 (EC 2.4.1.13)|
Version = NM_001063582.1 GI:115466895 GeneID:4340386|
Length = 5882 bp|
Definition = Oryza sativa Japonica Group Os06g0194900, complete gene.|
Source = Oryza sativa Japonica Group

ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
AP = Chromosome 6:4795629..4801510|
CDS = 4795896..4795943,4796041..4796179,4796291..4796535,4796666..4796984,4797324..4797548 ,4797637..4797803,4797886..4798002,4798087..4798260,4798357..4798452 ,4798535..4798751,4798847..4798965,4799115..4799307,4799391..4799542 ,4799632..4799752,4800164..4800258|
GCID = <gbrowseImage1>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage1>|
GSID = <gbrowseImage2>
name=NC_008399:4795629..4801510
source=RiceChromosome06
preset=GeneLocation
</gbrowseImage2>|
CDNA = <cdnaseq>atggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacacaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacgacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaa</cdnaseq>|
AA = <aaseq>MAAKLARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRH QLLAEFDALIEADKEKYAPFEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNVSEL AVEELSVSEYLAFKEQLVDGHTNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRH LSSKLFQDKESLYPLLNFLKAHNHKGTTMMLNDRIQSLRGLQSSLRKAEEYLMGIPQD TPYSEFNHRFQELGLEKGWGDCAKRVLDTIHLLLDLLEAPDPANLEKFLGTIPMMFNV VILSPHGYFAQSNVLGYPDTGGQVVYILDQVRALENEMLLRIKQQGLDITPKILIVTR LLPDAVGTTCGQRVEKVIGTEHTDILRVPFRSENGILRKWISRFDVWPFLETYTEDVA NEIMREMQAKPDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTKYPNSDIYLDKF DSQYHFSCQFTADLIAMNHTDFIITSTFQEIAGSKDTVGQYESHIAFTLPGLYRVVHG IDVFDPKFNIVSPGADMSVYFPYTEADKRLTAFHPEIEELLYSEVENDEHKFVLKDKN KPIIFSMARLDRVKNMTGLVEMYGKNAHLRDLANLVIVCGDHGNQSKDREEQAEFKKM YGLIDQYKLKGHIRWISAQMNRVRNGELYRYICDTKGVFVQPAFYEAFGLTVIEAMTC GLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFEKCKQDSTYWDNISQGG LQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASAVP LAVDGESTSK</aaseq>|
DNA = <dnaseqindica>5568..5615#5332..5470#4976..5220#4527..4845#3963..4187#3708..3874#3509..3625#3251..3424#3059..3154#2760..2976#2546..2664#2204..2396#1969..2120#1759..1879#1253..1347#aactatttattggtccttctggatctcggagaaaccctccattcgtttgctcgtctctgaccaccattgggtatgttgcttccattgccaaactgttcccttttacccataggctgattgatcttggctgtgtgattttttgcttgggtttttgagctgattcagcggcgcttgcagcctcttgatcgtggtcttggctcgcccatttcttgcgattctttggtgggtcgtcagctgaatcttgcaggagtttttgctgacatgttcttgggtttactgctttcggtaaatctgaaccaagaggggggtttctgctgcagtttagtgggtttactatgagcggattcggggtttcgaggaaaaccggcaaaaaacctcaaatcctcgacctttagttttgctgccacgttgctccgccccattgcagagttctttttgcccccaaatttttttttacttggtgcagtaagaatcgcgcctcagtgattttctcgactcgtagtccgttgatactgtgtcttgcttatcacttgttctgcttaatcttttttgcttcctgaggaatgtcttggtgcctgtcggtggatggcgaaccaaaaatgaagggttttttttttttgaactgagaaaaatctttgggtttttggttggattctttcatggagtcgcgaccttccgtattcttctctttgatctccccgcttgcggattcataatattcggaacttcatgttggctctgcttaatctgtagccaaatcttcatatctccagggatctttcgctctgtcctatcggatttaggaattaggatctaactggtgctaatactaaagggtaatttggaaccatgccattataattttgcaaagtttgagatatgccatcggtatctcaatgatacttactaaaacccaacaaatccatttgataaagctggttcttttatccctttgaaaacattgtcagagtatattggttcaggttgatttattttgaatcagtactcgcactctgcttcgtaaaccatagatgctttcagttgtgtagatgaaacagctgtttttagttatgttttgatcttccaatgcttttgtgtgatgttattagtgttgatttagcatggctttcctgttcagagatagtcttgcaatgcttagtgatggctgttgactaattattcttgtgcaagtgagtggttttggtacgtgttgctaagtgtaacctttctttgcagttcctgaaattgagtcatggctgccaagctagctcgcctccacagtctccgcgaacgcctcggtgccaccttctcgtctcatcccaatgagttgattgcactcttctctaggtgaagatctcaatataatatcttgtgtcttcttggaacctgctactaattgttttgagttttcaatgattttatccatacagttggttagttcttagtcaacccagatgtgaaacgataacatgttagtatgttacaaattcttagctgacaaggttatgcatctctttagttttaatgatggtatctataataatctgtgatgagctctaacatttttgtcttgagtcatgaatcttagatgatgttgaaatattgtttgttcatagctagattaagtacagatcatgtggtcttaaaaataagaagtaaaaatcaggtttatgtggtagtgccattaacatagttcagccgcagaaaggatgtgagatctaagcattggttctatttcttaaatatcactctatgcaggtatgttaaccagggaaagggaatgctccagcgtcaccagctgcttgcggagttcgatgccttgatcgaagctgacaaagagaaatatgctccctttgaagacattctccgggctgctcaggtaaatttggcaatgagccaggatggttatgatagtcaactcaaagtcaaataccagtctttctaacttacttccttgactgtatgtaggaagccattgtgctgccgccctgggttgcactggccatcaggccaaggcctggtgtctgggactacattcgggtgaatgtaagtgagttggcagtggaagagctgagtgtttctgagtacttggcattcaaggaacagcttgttgatggacagtaagtaccagaacgacgatttgcagattataactgaagcaaaataacagtgactgaattttacttctgtttttcttaaacagcaccaacagcaactttgttcttgagcttgattttgagcccttcaatgcctccttcccgcgcccgtccatgtccaagtccatcggaaatggggtgcagttccttaaccgtcacctgtcgtccaagttgttccaggacaaggagagcctctaccccttgctgaacttcctgaaagcccataaccacaagggcacggtaagcttccaatttgtggaacataccaaatgcttttatactagatgttttttcttgtgtgaattgctttaaatgttagtcctttagaaacccacttgacattctctgtaagttccaccaaatgacgggatccgaattttctattacagacaatgatgctgaatgacagaattcagagccttcgtgggctccaatcatcccttagaaaggcagaagaatatctgatgggcattcctcaagacacgccctactcggagttcaaccacaggtgattactttattatttctccttatcatatagcccatgttatactactgcaaatggaataattccttacaatgtgattcatgatttgaatacaggttccaagagctcggtttggagaagggttggggtgactgtgcaaagcgtgtgcttgacaccatccacttgcttcttgaccttcttgaggcccctgatccggccaacttggagaagttccttggaactattccaatgatgttcaatgttgttatcctgtctccgcatggatactttgcccaatccaatgtgttgggataccctgatactggtggtcaggtagagctttttaacaaattttccccttatatacagtacttcaaatatttctagttttgacctgttattctgttgtttgcaggttgtgtacattttggaccaagtccgcgctttggagaatgagatgcttttgaggatcaagcagcaaggccttgatatcacacctaagatcctcattgtatgttcaagatgttggtcttgaatgtttcaagttttaaacacttctcagtgcgaattgtagagtaacgtgcggacatgattttacttgatgcaggtaaccaggctgttgcctgatgctgttggtactacatgcggccagcgtgtggagaaggttattggaactgagcacactgacattcttcgtgttccattcaggagtgagaatggtatcctccgcaagtggatctcccgttttgatgtctggccattcctggaaacatacactgaggtaaatggtttatctgacaacgtccttcagagctgagcacaaaccatgaattctgagagctcatctttttttttttggttgcaggatgttgcaaacgaaattatgagggaaatgcaagccaaacctgatctcatcattggcaattacagtgatggaaaccttgttgccactctgctggctcacaaattaggagttacccaggtatgtttggttgtaaaccactcctttaaatatttctttcattaagtcaattacttatgatttctcttctacatggatacagtgtaccattgctcatgccttggagaaaaccaaataccccaactcagacatatacttggacaagtttgacagccagtaccacttctcatgccaattcactgctgatcttatcgccatgaatcacactgatttcatcatcaccagtacattccaagaaattgctggaaggtaaaattttctaaagagcagtagaatattgctgcgttgtgccaacacctaaacgggtatctgatgtttgtttcctctgtttcttcagcaaggacactgtggggcagtatgaatcacacattgcattcacccttcctgggctttaccgagttgtgcatggcatagatgtttttgatcccaagttcaacattgtctctcctggagctgacatgagtgtctacttcccgtacaccgaggctgacaagaggctcactgctttccaccctgaaattgaggagcttctctacagtgaagtcgagaacgatgaacacaagtgagtactgaactgctggttccgtccctgttattcacctaccatgtaacaaataattaaacactgatttgtgtgtttggtctagctttggctcttcagtctactaagtgccatgcactagaactttgtagatttgaactgcaattcttaattttctcactccttagtagaccacaataataattattgtgtctgacccagctagtttacaatagtgacttcaatgcttgttttttccccttgaacaattgtttactttctagcagtggagtttggcatctaaacttgaatacacgctgttcttttttgtgtataaaatgtttatttaatgttctgcaggtttgtattgaaggacaagaacaagccaatcatcttctccatggctcgtcttgaccgagtgaagaacatgacaggtctggttgagatgtatggtaagaatgcacatctcagggatttggcaaaccttgtgattgtttgtggtgaccacggcaatcagtccaaggacagggaggagcaggctgagttcaagaagatgtacggtctcattgaccagtacaagttgaaggggcatatccgctggatctcagctcagatgaaccgtgttcgtaacggggagttgtaccgatacatttgtgacaccaagggagtctttgtccaggtatatacatacgctgtcatattctactgctatatttctattctgagcttccagtagcaaatgatttctactatttgtaaagaacagccttgtgcttgagactaaaactgcttttaaaattgttttttagcctgcattctatgaagcgtttggtctgactgtcatcgaagccatgacatgtggtttgccaacaatcgcaacatgccatggtggccctgctgagattattgttgatggggtgtctggtctgcacattgatccttaccacagtgacaaggctgctgatatcttggtcaacttctttgagaagtgcaagcaggattcaacctactgggacaatatttcacagggaggtctgcagaggatttacgagaagtatgtatttcatgatctgcaaatatataacattttttttttacgattgacatataactgtagaaatcatgtgtccaatctatattgatcctacaaatttggacttgtcaggtacacctggaagctgtactctgagaggctgatgaccttgactggtgtatacggattctggaagtacgtaagcaaccttgagaggcgcgagactcgccgttacattgagatgttctatgctctgaaataccgcagcctggtaagttactctcgctgttcaacagcttgttaatttcagtattattgcactgagcatatctctgaattctgttgctacatttctttgttgaatccaggccagcgccgtcccattggctgtcgatggagagagcacatccaagtaatggaggggaaaatatgcatcttcagcaggagaagccgtcagctgcattcgaatttgataattttctgtagttgtcatttggcatccatgtttgccatggatgtgtactatgtctaaggtttcagtacttttgcgagattttgggcagtgcttgcctcaaataaacgccggttcctggtgtttatcgttcagaattcaatgcaatgttttattgccgcgcttcctcaatctgtcatgttgaatgaccactccctagtgccctactgtt</dnaseqindica>|
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001063582.1 RefSeq:Os06g0194900]|
}}
[[Category:Genes]]
[[Category:Japonica mRNA]]
[[Category:Oryza Sativa Japonica Group]]
[[Category:Japonica Genes]]
[[Category:Japonica Chromosome 6]]
[[Category:Chromosome 6]]

Os06g0194900

2014-05-17T15:52:52Z

Yangmei li: /* Expression */