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Expression of OsWRKY89was induced by phytohormones and various stresses. (A)RNA gel-blot analysis ofOsWRKY8 9 expression in rice. Total RNA was isolated from leaves of rice seedlings subjected to different treatments at different time points. Equal loading of 10lg total RNA to each lane was checked by ethidium bromide staining. P-labeled cDNAs of OsWRKY89, OsBBPI, or PAL fragments were used for hybridizations. Rice seedlings were treated with phytohormones ABA (100lM) and MeJA (100lM), or with UV-B and mechanical wounding. MES buffer, or 0.1% methanol treatment was used as the controls. (B–F) Histochemical staining of the transgenic rice seedlings carrying theOsWRKY89 promoter-gusfusion construct. The four-day-old rice seedling (B) and its section (D), the four-day-old rice seedling treated with UV-B for 15 min and sampled 6 h after the treatment (C) and its section (E) were stained. (F)GUS staining of a 3-week-old leaf. (G)transverse section of a root, showing expression ofGUSin the epidermal cells. Bars = 0.5 cm in (B, C) and 0.1 cm in (D–G).