File:图片8n3.png

Os8N3is amemberof theMtN3family. (A)Os8N3is expressed as a 1.6-kb messenger RNA. Os8N3 is shown as represented by the full-length cDNA AK070510. Exons are represented by the solid black bars, and introns are indicated above the bars. Numbers indicate the number of base pairs of nucleotides. The first and last numbers for exons indicate the predicted 5� and 3� untranslated regions, respectively. 5� and 3� probes used throughout the study for hybridization (see Results) are indicated below the diagram. (B)Os8N3product is predicted to encode a 307-aa integral membrane protein. The underlined region is a predicted uncleaved leader peptide. Eight predicted transmembrane regions are in bold. (C) Alignment of representative related proteins of the MtN3 family. Sequences were aligned by using CLUSTALW (38) and displayed by using BOXSHADE (www.ch.embnet.org�software�BOX�form.html). Protein sequence files: Medicago truncatula nodulin protein CAA69976 (MtN3), Arabidopsis MtN3-like protein AAL47380 (Arab); recombination activating gene 1 gene activation protein AAH14292(Mouse), Drosophila melanogaster saliva proteinAAD03390(Fly),and Caenorhabditis elegans protein AAC02609 (Nema).