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Figure 1. Identification of OsMYB3R-2 transgenic rice and its expression pattern. A, Northern-blot assay of rice transgenic plants. Total RNA isolated from wild-type (WT) or transformed plants underwent hybridization with a [a-32P]dCTP-labeled probe of OsMYB3R-2 cDNA as described in “Materials and Methods.” B, Real-time RTPCR of the expression of OsMYB3R-2 in antisense lines. C and D, Southernblot assay of transformed rice plants. Genomic DNA isolated from wild-type or transformed plants was digested with EcoRI (E) or HindIII (H). The blot was hybridized with the open reading frame of the GUS gene labeled with [a-32P]dCTP. OL3, OL5, OL7, and OL8 and AL1, AL2, AL4, and AL5 represent overexpression (O) and antisense (A) lines of OsMYB3R-2 transgenic rice. E, Expression pattern of OsMYB3R-2 in vivo. GUS staining shows expression pattern of OsMYB3R-2 in vivo in various tissues from the T1 generation of OsMYB3R-2 promoter::GUS transgenic rice. a, Root; b, young internode; c, mature internode; d, node; e, mature leaf; f, lamina joint; g, leaf sheath; h, flower; i, immature seed.