File:AVR1-CO39 Interacts Physically with RGA5.jpg
(A) Screening of a rice yeast two-hybrid cDNA library with AVR1-CO39 identified three different clones for AVR1-CO39 Interactor 1 (ACI1-L, -M, and -S).Interactions were assayed on synthetic triple dropout (TDO) medium lacking Trp, Leu, and His and supplemented with 10 mM 3-amino-1,2,4-triazole(3AT). Autoactivation of BD:AVR1-CO39 and AD:ACI1 constructs was testedusingempty pGADT7-AD (Empty-AD) and empty pGBKT7-BD (Empty-BD)vectors. Photos show yeast colonies after 4 d of growth. (B) ACI1-L, -M, and -S align to the 39 extremity of the RGA5 gene located adjacent to the RGA4 gene on chromosome 11 (Chr. 11) of rice varieties Sasanishiki and CO39. nt, nucleotides.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 04:37, 3 June 2014 | | 998 × 764 (147 KB) | Tangbo13 (talk | contribs) | (A) Screening of a rice yeast two-hybrid cDNA library with AVR1-CO39 identified three different clones for AVR1-CO39 Interactor 1 (ACI1-L, -M, and -S).Interactions were assayed on synthetic triple dropout (TDO) medium lacking Trp, Leu, and His and supplem |
| 04:37, 3 June 2014 |  | 998 × 764 (147 KB) | Tangbo13 (talk | contribs) | (A) Screening of a rice yeast two-hybrid cDNA library with AVR1-CO39 identified three different clones for AVR1-CO39 Interactor 1 (ACI1-L, -M, and -S).Interactions were assayed on synthetic triple dropout (TDO) medium lacking Trp, Leu, and His and supplem |
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