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(C) Phylogenetic analysis revealed that OsRad21-4 was grouped with Rec8-like proteins from other species and formed a clade with Arabidopsis Syn1. The tree was derived from the multiple alignments of N-terminal regions of OsRad21-4 and other Rad21/Rec8 sequences with use of Clustal W plus a neighbor-joining feature with removal of most distance sequences. These sequences are shown as follows: OsRad21–1 and OsRad21-4 (this study, Oryza sativa, accession No. AY28893 and AY371049); Syn1, Syn2 and Syn3 (Arabidopsis thaliana, accession No. AF080619, AF281154 and AF281155); HsRad21 and HsRec8 (Homo sapiens, accession No. NM_006265 and NM_005132); MmRad21 and MmRec8 (Mus muculus, accession No. NP_033035 and AAF69524); CeRec8 (Caenorhabditis elegans, accession No. CAB05309); DmRad21 (Drosophila melanogaster, accession No. NM_143900); XRad21 (Xenopus laevis, accession No. AF051786); and SpRec8 (Schizosaccharomyces pombe, accession No. AB018077). Bootstrap values, evaluated for 100 bootstrap trails, are shown at branch points