File:FiG2.png

Product and function of the OsWRKY13 gene. A, Schematic diagrams of conserved motifs of OsWRKY13. NLS = nuclear localization signal, WRKY =WRKY motif, and zinc finger = zinc-finger motif. Conserved sequences of these structures are presented, and numbers indicate the amino acid position of each structure in OsWRKY13. B, OsWRKY13-overexpressing plants show enhanced resistance to Xanthomonas oryzae pv. oryzae PXO61 at both the seedling and booting stages. The T2 transgenic plants (1-1, 7-2, 18-10) are from T0 plants D11UM1, D11UM7, and D11UM18. 7-2N is a transgene-negative plantsegregated in the D11UM7-2 family. C, Expression analysis of OsWRKY13 in noninoculated T0 transgenic plants, wild type, mock (water)-inoculated Minghui 63 (donor of OsWRKY13, M), and PXO61-inoculated Minghui 63 (P) by RNA gel blot analysis. D, Enhanced resistance to PXO61 cosegregates with overexpression of OsWRKY13 in T2 families D11UM1-4 and D11UM7-2. E, Growth of PXO61 in leaves of T3 OsWRKY13-overexpressing (D11UM1) and wild-type plants. Bacterial populations were determined from three leaves at each timepoint by counting CFUs. Similar results were obtained in two independent biological experiments. F, Resistance of OsWRKY13-overexpressing plants for Blast. The T1 negative (D11UM18-1N) and positive (D11UM18-4) plants were from T0 plant D11UM18. Similar results were obtained in two independent biological experiments. ck = Mudanjiang 8 (wild type).