File:Fig.3w.png
Figure 3w. A. Immunoblot analysis of OsMPK5 in transgenic and wild-type (WT) plants after the BPH had been feeding for between 0 and 96 h. Protein (50 mg) was separated by SDS-PAGE, electroblotted, and probed with rabbit OsMPK5 polyclonal antibody (1:1.000). After incubation with goat anti-rabbit IgG (1:10.000) conjugated to alkaline phosphatase, the complex was visualized using 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium solution. Rice HSP82 (Q69QQ6) mouse monoclonal antibody (1:10,000) was used as a loading control. B, Comparative protein levels of OsMPK5 in wild-type and transgenic plants from three replicates. The western-blot signals of OsMPK5 were analyzed with Quantity One (Bio-Rad) and are shown with a histogram.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 08:19, 26 May 2014 | | 559 × 265 (71 KB) | WilliamE (talk | contribs) | Figure 3w. A. Immunoblot analysis of OsMPK5 in transgenic and wild-type (WT) plants after the BPH had been feeding for between 0 and 96 h. Protein (50 mg) was separated by SDS-PAGE, electroblotted, and probed with rabbit OsMPK5 polyclonal antibody (1:1.00 |
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