File:Figure2. RSS3 and JAZ9 together Suppress bHLH094-Mediated Gene Activation.jpg

(A) bHLH094 enhances the activity of the gene promoter containing E-box (CANNTG).

(B) A combinational expression of RSS3 and JAZ9 suppresses the bHLH094-mediated gene activation driven by the promoter containing G-box (CACGTG). Effector plasmids containing bHLH094, RSS3, RSS3_D21, and JAZ9 driven by the CaMV 35S promoter were introduced into rice protoplasts, concomitant with a reporter plasmid containing a triplicate E-box (A) or G-box (B) fused to the basal promoter-fLUC (reporter). fLUC activity was measured and normalized with that of rLUC. Empty vector was added to equalize the amount of plasmids transfected in each combination. Mean ± sd; n = 3 to 8. Asterisks indicate a Student’s t test P value < 0.05 in (A). Statistical differences were determined by one-way ANOVA followed by Tukey posthoc analysis, and different letters indicate significant differences (P value < 0.05) in (B). fLUC, firefly luciferase; rLUC, renilla luciferase.