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(A) Dual immunolocalization of WT scattered chromosomes with REC8 (red) and HEI10 (green) antibodies. Scale bar: 5 mm. (B) Dual immunolocalization of CENH3 and HEI10 at late pachytene. (C) No HEI10 signals were detected at metaphase I, chromosomes are stained with DAPI (blue). Scale bars: 5 mm. (D) Histogram of the observed distances between two adjacent HEI10 bright foci on the shortest chromosomes (percentages of the length of the synaptonemal complex). The gray bars show the observed relative frequencies of inter-HEI10 foci distances. The red curve presents the best fit of the distance to the gamma distribution. n is the interference parameter in the gamma model. v ̂ is the n value (estimated SE) for which the best fit of the observed distances to the gamma model was got. Here, the estimatev ̂ is 8.39, which indicates a strong interference among HEI10 bright foci on the shortest chromosome in rice (if there is no interference, n is 1).
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 10:12, 4 June 2014 | | 391 × 800 (71 KB) | Simon (talk | contribs) | (A) Dual immunolocalization of WT scattered chromosomes with REC8 (red) and HEI10 (green) antibodies. Scale bar: 5 mm. (B) Dual immunolocalization of CENH3 and HEI10 at late pachytene. (C) No HEI10 signals were detected at metaphase I, chromosomes are st |
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