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Figure 7. Subcellular localization. A to D, Mesophyll protoplasts prepared from young seedlings were cotransformed with fusion constructs Pubi::OsGT1-GFP and P35S::ManI-RFP. Images observed under GFP channel (A) and RFP channel (B) were merged (C); D is a bright-field image. E to H, Onion epidermal cells were cobombarded with fusion constructs. The GFP channel image (E) and RFP channel image (F) were merged (G); H is a bright-field image. I, GFP image of mesophyll cells xpressing control construct Pubi::GFP. J, GFP image of onion epidermal cell expressing control construct P35S::GFP. Bars = 5 mm for mesophyll protoplasts and 50 mm for onion cells.