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Figure 7. Expression patterns of cyclin genes in the OsMYB3R-2 transgenic rice, cold response and survival ratio, and cellular free Pro in the OsCycB1;1 transgenic rice lines. A, Expression patterns of cyclin-responsive genes in wild-type (WT) and OsMYB3R-2 transgenic rice under room temperature (25℃) or cold treatment (4℃) for 24 h. The method of obtaining seedlings at the same stage is described in “Materials and Methods.” Tubulin was used as an internal control. AL1 and AL4, OsMYB3R-2- antisense lines; OL5, OL7, and OL8, OsMYB3R-2-overexpressing lines. The experiments on the response of cyclin genes to cold were repeated at least twice. B, The response of the OsCycB1;1 transgenic rice lines to cold stress. AT, Grown for 1 week after the cold treatment (3℃) for 72 h; BT, before the cold treatment (3℃), which is a control without cold treatment; OE, OsCycB1;1- overexpressing lines; RNAi, OsCycB1;1-RNAi lines. C, The determination of cellular free Pro in OsCycB1;1 transgenic rice lines. AT, After the cold treatment (2℃) for 24 h; BT, before the cold treatment (2℃); FW, fresh weight of materials. Error bars in B and C show SE and are from three independent replications. Data represent means and SE of experiments performed in triplicate.