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Figure 5 Excitation/emission spectrum analysis of the BiFC signal and fluorescent proteins expressed in the transformed rice protoplasts. (a) The emission signal peak of MTS-mOrange, excited by 543-nm laser, was detected at 565 nm. (b) OsCOX11-CFP was excited by 454-nm laser and the emission signal peaks were detected at 480 nm and 505 nm. (c) When excited by 488-nm laser, the emission signal peak of the reconstituted YFP (yellow fluorescent protein) by BiFC of MTS-SPYNE-WA352218-352and MTS-SPYCE-OsCOX11 wasdetected at 522 nm, indicating that the BiFC signal did notoverlap with those of MTS-mOrange and OsCOX11-CFP.