File talk:Figure 1 - Molecular characterization of OsDWARF4 and OsDWARF4L1-D11..jpg

(a) Phylogenetic relationships among brassinosteroid-biosynthetic cytochrome P450s of rice, A. thaliana and tomato. Bar, 0.1 amino-acid substitutions per site. (b) Genomic organization of the OsDWARF4 gene. Boxes represent exons, and lines separating boxes represent introns. The arrowhead indicates the site of Tos17 insertion in osdwarf4-1. (c) Relative positions of the major domains in OsDWARF4. All of the major domains found in the cytochrome P450 superfamily are conserved in OsDWARF4. The arrowhead indicates the site of Tos17 insertion in osdwarf4-1. (d) Expression of OsDWARF4 and OsDWARF4L1/D11 in various organs of wild-type rice. The gene encoding Histone H3 was used as a control. (e) Feedback regulation of OsDWARF4 and OsDWARF4L1/D11 in brassinolide-treated wild-type plants (left) and brassinosteroid-related mutants (right). Values under each panel indicate relative amounts of each transcript. (f) Function of recombinant CYP90B2/OsDWARF4 and CYP724B1/D11 proteins. Campesterol (CR) was incubated with each recombinant protein and analyzed by GC-MS. Inset shows that the mass spectrum of the trimethylsilyl ester of each metabolite coincided with that of the trimethylsilyl ether of synthesized (22S)-22-hydroxycampesterol (22-OHCR).