AB013448 - Revision history

Zhennan at 07:24, 25 June 2016

2016-06-25T07:24:05Z

Jirongli: /* Expression */

2014-06-08T09:24:32Z

‎Expression

Jirongli: /* Evolution */

2014-06-08T09:23:09Z

‎Evolution

Jirongli: /* Expression */

2014-06-08T09:22:05Z

‎Expression

Jirongli: /* Function */

2014-06-08T09:19:57Z

‎Function

Jirongli: /* Function */

2014-06-08T09:16:37Z

‎Function

Jirongli: /* Expression */

2014-06-08T09:15:31Z

‎Expression

Jirongli: /* Evolution */

2014-06-08T09:13:57Z

‎Evolution

Jirongli: /* Structured Information */

2014-06-08T02:58:41Z

‎Structured Information

Jirongli: /* Structured Information */

2014-06-08T02:57:33Z

‎Structured Information

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 ==Annotated Information==
 ===Function===
 Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (<0.5 and <1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.【5】
 Link = [http://www.ricedata.cn/reference/list/1419.htm]|
 ===Expression===

@@ Line 21: / Line 21: @@
 ===Expression===
-The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【2】
+The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【3】
-Link = [http://www.ricedata.cn/reference/list/1419.htm]|
+Link = [http://www.ricedata.cn/reference/list/507.htm]|
 ===Evolution===

@@ Line 25: / Line 25: @@
 ===Evolution===
-Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】
+Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.【1】Link = [http://www.ricedata.cn/reference/list/13405.htm]|
 You can also add sub-section(s) at will.

@@ Line 22: / Line 22: @@
 ===Expression===
 The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.【2】
 ===Evolution===

@@ Line 4: / Line 4: @@
 Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (<0.5 and <1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.【5】
 ====常规信息====
 Pib ，水稻稻瘟病抗性基因，来自品种"BL1"，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。
@@ Line 17: / Line 18: @@
 ====分子标记辅助选择育种====
 利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。
 ===Expression===

@@ Line 56: / Line 56: @@
 AP = Chromosome 2:2293..9202|
 CDS = 2293..9202|
-GCID = <gbrowseImage1>
 AA = <aaseq>MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE
                       LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL