Zhaocf at 13:29, 1 June 2014

2014-06-01T13:29:55Z

Zhaocf: Expression pattern of OsC6. A, Spatial and temporal expression analyses of OsC6 by qRT-PCR. Rice Actin1 (OsActin1) expression was used as a control. Total RNA was isolated from rice tissues: root, shoot, leaf, glume, lemma, palea, pistil at stage 13, and

2014-06-01T13:26:02Z

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Expression pattern of OsC6. A, Spatial and temporal expression
analyses of OsC6 by qRT-PCR. Rice Actin1 (OsActin1) expression
was used as a control. Total RNA was isolated from rice tissues: root,
shoot, leaf, glume, lemma, palea, pistil at stage 13, and anthers at
various stages (St 8, stage 8; St 9, stage 9; St 10, stage 10; St 11, stage 11;
St 13, stage 13). B, GUS expression in anthers at stage 10 of wild-type
(WT; left) and tdr (right) background. C, GUS expression was visible in
immature anthers at different stages (from stage 8 to stage 13). D, GUS
expression (arrowhead) in the tapetal cells at stage 10. The section was
counterstained by Safranin O. E to H, In situ analyses of OsC6. E, A
wild-type anther at stage 8 showing no OsC6 expression. F, A wild-type
anther at stage 10 showing stronger OsC6 expression in tapetal cells. G,
The expression of OsC6 decreased at stage 11. H, Successive section to
that shown in F, probed with the OsC6 sense probe. A, Anther; E,
epidermis; L, lemma; Msp, microspore; P, palea; T, tapetum; Tds,
tetrads. Bars = 2 mm in B, 100 mm in C, and 20 mm in D to H.

← Older revision		Revision as of 13:29, 1 June 2014
Line 1:		Line 1:
−	Expression pattern of OsC6. A, Spatial and temporal expression	+	Expression pattern of OsC6. A, Spatial and temporal expression analyses of OsC6 by qRT-PCR. Rice Actin1 (OsActin1) expression was used as a control. Total RNA was isolated from rice tissues: root, shoot, leaf, glume, lemma, palea, pistil at stage 13, and anthers at various stages (St 8, stage 8; St 9, stage 9; St 10, stage 10; St 11, stage 11; St 13, stage 13). B, GUS expression in anthers at stage 10 of wild-type (WT; left) and tdr (right) background. C, GUS expression was visible in immature anthers at different stages (from stage 8 to stage 13). D, GUS expression (arrowhead) in the tapetal cells at stage 10. The section was counterstained by Safranin O. E to H, In situ analyses of OsC6. E, A wild-type anther at stage 8 showing no OsC6 expression. F, A wild-type anther at stage 10 showing stronger OsC6 expression in tapetal cells. G, The expression of OsC6 decreased at stage 11. H, Successive section to that shown in F, probed with the OsC6 sense probe. A, Anther; E, epidermis; L, lemma; Msp, microspore; P, palea; T, tapetum; Tds, tetrads. Bars = 2 mm in B, 100 mm in C, and 20 mm in D to H.
−	analyses of OsC6 by qRT-PCR. Rice Actin1 (OsActin1) expression
−	was used as a control. Total RNA was isolated from rice tissues: root,
−	shoot, leaf, glume, lemma, palea, pistil at stage 13, and anthers at
−	various stages (St 8, stage 8; St 9, stage 9; St 10, stage 10; St 11, stage 11;
−	St 13, stage 13). B, GUS expression in anthers at stage 10 of wild-type
−	(WT; left) and tdr (right) background. C, GUS expression was visible in
−	immature anthers at different stages (from stage 8 to stage 13). D, GUS
−	expression (arrowhead) in the tapetal cells at stage 10. The section was
−	counterstained by Safranin O. E to H, In situ analyses of OsC6. E, A
−	wild-type anther at stage 8 showing no OsC6 expression. F, A wild-type
−	anther at stage 10 showing stronger OsC6 expression in tapetal cells. G,
−	The expression of OsC6 decreased at stage 11. H, Successive section to
−	that shown in F, probed with the OsC6 sense probe. A, Anther; E,
−	epidermis; L, lemma; Msp, microspore; P, palea; T, tapetum; Tds,
−	tetrads. Bars = 2 mm in B, 100 mm in C, and 20 mm in D to H.

File:Expression pattern of OsC6.jpg - Revision history

Zhaocf at 13:29, 1 June 2014

Zhaocf: Expression pattern of OsC6. A, Spatial and temporal expression analyses of OsC6 by qRT-PCR. Rice Actin1 (OsActin1) expression was used as a control. Total RNA was isolated from rice tissues: root, shoot, leaf, glume, lemma, palea, pistil at stage 13, and