Mmlin66: Figure 6. Pollen viability of OsRad21-4-deﬁcient lines was severely affected. Intact anthers in wild type (A) and deﬁcient lines (B) were stained with TTC solution and spread gently, showing that the deﬁcient-line anthers contained a decreased amoun

2014-05-22T13:04:47Z

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Figure 6. Pollen viability of OsRad21-4-deﬁcient lines was severely affected. Intact anthers in wild type (A) and deﬁcient lines
(B) were stained with TTC solution and spread gently, showing that the deﬁcient-line anthers contained a decreased amount of
pollen grains as compared with wild type anthers. Viability of mature pollen grains in wild type (C and E) and deﬁcient lines
(D and F) were examined on I
-KI (C and D) or TTC (E and F) staining. Pollen grains ﬁlled with starch granule showed dark
blue, but those with little or no starch showed weak yellow during I
2
-KI staining. Viable pollen grains showed red, with yellow for
nonviable ones in TTC staining. Scale bars in (A) = 250 lm for (A) and (B), and in (C) =80 lm for (C)–(F).

File:F6.png - Revision history

Mmlin66: Figure 6. Pollen viability of OsRad21-4-deﬁcient lines was severely affected. Intact anthers in wild type (A) and deﬁcient lines (B) were stained with TTC solution and spread gently, showing that the deﬁcient-line anthers contained a decreased amoun