Mmlin66: Figure 8. Abnormal meiotic chromosome behavior in microsporocytes of OsRad21-4-deﬁcient lines. Meiosis chromosome spreads were prepared from ﬁxed anthers from the deﬁcient lines (R1 and R5, data are shown only for R5). (A) leptonema; chromatins were

2014-05-22T13:28:08Z

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Figure 8. Abnormal meiotic chromosome behavior in microsporocytes of OsRad21-4-deﬁcient lines. Meiosis chromosome spreads
were prepared from ﬁxed anthers from the deﬁcient lines (R1 and R5, data are shown only for R5). (A) leptonema; chromatins
were compacted into a entangled mass. (B) zygonema; chromosomes showed defects in condensation, with most regions being
highly condensed in agglomeration. (C) and (D) pachynema, chromosomes were highly condensed compared with those of the wild
type and unsynapsed homologues, with detached regions distal to centromeres and chromosome fragments visible (arrows); (E) diplonema;
homologues precociously segregated into univalents. (F) diakinesis; univalent (not marked), bivalents (concaved arrows) and chromosome
fragments (arrows) are visible. (G) late diakinesis; homologues precociously segregated into 24 univalents. (H) late diakinesis; �50 stained
bodies, reﬂecting severe chromosome fragmentation. (I) and (J) metaphase I; chromosomes (arrows) did not align on equator. (K) anaphase
I; chromosome bridging was formed by lagging chromosomes linking two segregating groups of chromosomes (arrows). (L) anaphase
I; chromosomes segregated randomly. (M) telophase I; chromosomes cannot be segregated into each pole (arrows). (N) dyad with
lost chromosomes and unequal nuclei. (O) dyad with 1 cell having 2 unequal nuclei. (P) dyad with unequal nuclei and cytoplasm. (Q)
metaphase II, 2 daughters lost synchronization to initiate transition of metaphase II to anaphase II. (R) tetrad stage; misorientation of
spindles in 2 daughters resulted in cell division occurring in 1 daughter but not the other. (S) tetrad stage; 4 cells with unequal cytoplasm
and chromosome complements. Bar in (A) = 20 lm for (A)–(S).

File:F8.png - Revision history

Mmlin66: Figure 8. Abnormal meiotic chromosome behavior in microsporocytes of OsRad21-4-deﬁcient lines. Meiosis chromosome spreads were prepared from ﬁxed anthers from the deﬁcient lines (R1 and R5, data are shown only for R5). (A) leptonema; chromatins were