Yang Yingying: An ATPase activity is associated with XB24 and effects XA21 auto- phosphorylation. (A) ATPase activity assay on purified Ntap-XB24 and Ntap- XB24S154Aprotein from transgenic plants. The same amount of proteins was used. (Left) Representative autoradiogram

2014-06-06T14:03:06Z

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An ATPase activity is associated with XB24 and effects XA21 auto-
phosphorylation. (A) ATPase activity assay on purified Ntap-XB24 and Ntap-
XB24S154Aprotein from transgenic plants. The same amount of proteins was
used. (Left) Representative autoradiogram. (Right) Quantitative results of
three independent experiments. ATP hydrolysis was quantified based on
radioactivity of the reaction product Pi. Error bars indicate SDs. (B) Effects of
XB24 on XA21 autophosphorylation. In vitro autophosphorylation assays
were performed on GST-XA21K668 and GST-XA21K668K736E, respectively, in
the presence of the purified His-XB24 protein. (C) Effects of XB24 ATPase on
XA21 autophosphorylation. An in vitro autophosphorylation assay was
performed on GST-XA21K668 in the presence of the same amount of rice-
expressed Ntap-XB24 or Ntap-XB24S154A. (Upper) Representative auto-
radiogram. (Lower) Quantitative results (mean + SD) from three independ-
ent experiments. CK, control provided using autophosphorylation assay on
GST-XA21K668 in the absence of XB24. The autophosphorylation level from
CK was arbitrarily set as “1.”

File:Fig. 2B.png - Revision history

Yang Yingying: An ATPase activity is associated with XB24 and effects XA21 auto- phosphorylation. (A) ATPase activity assay on purified Ntap-XB24 and Ntap- XB24S154Aprotein from transgenic plants. The same amount of proteins was used. (Left) Representative autoradiogram