Tracy: Figure 3. Phenotypes of pollen grains. A to D, Auramine O staining of grains from segregating wild-type (A and C) and OsGT1/ osgt1-1 (B and D) plants at stage 12 observed under bright-field (A and B) and fluorescence (C and D) microscopy. E to H, Calcoflu

2014-06-04T05:10:37Z

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Figure 3. Phenotypes of pollen grains. A to D, Auramine O staining of grains from segregating wild-type (A and C) and OsGT1/ osgt1-1 (B and D) plants at stage 12 observed under bright-field (A and B) and fluorescence (C and D) microscopy. E to H, Calcofluor white staining of pollen grains from segregating wild-type (E and G) and OsGT1/osgt1-1 (F and H) plants at stage 12 observed under bright-field (E and F) and fluorescence (G and H) microscopy. I to P, DAPI staining of pollen grains from segregating wild-type (I, K, M, and O) and OsGT1/osgt1-1 (J, L, N, and P) plants at stage 12 (I–L) and stage 11 (M–P) plants observed under bright-field (I, J, M, and N) and fluorescence (K, L, O, and P) microscopy. Arrows indicate nucleus. DP, Defective pollen. Bars = 20 mm.

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Tracy: Figure 3. Phenotypes of pollen grains. A to D, Auramine O staining of grains from segregating wild-type (A and C) and OsGT1/ osgt1-1 (B and D) plants at stage 12 observed under bright-field (A and B) and fluorescence (C and D) microscopy. E to H, Calcoflu