Tracy: Figure 5. Expression pattern of OsGT1. A, Quantitative RT-PCR analyses in various organs. Transcript levels were normalized to UBIQUITIN and calculated by the comparative cycle threshold method. Error bars indicate SD. 7DR, Seven-day-old seedling roots; 7

2014-06-04T05:12:35Z

New page

Figure 5. Expression pattern of OsGT1. A, Quantitative RT-PCR analyses in various organs. Transcript levels were normalized to UBIQUITIN and calculated
by the comparative cycle threshold method. Error bars indicate SD. 7DR, Seven-day-old seedling roots; 7DS, 7-dold seedling shoots; SAM, shoot apical meristem; ML, mature leaves; IF, immature panicles (2 cm long); 3DAPS, seeds at 3 d after pollination; stages 7 to 12, anthers at different developmental stages. B, Quantitative real-time RT-PCR analyses of OsGT1 in wild-type and
heterozygous anthers at mitotic stage.
C, GUS expression in a spikelet harboring
an OsGT1 promoter::GUS construct.
An, Anther; Ca, carpel; Pa, palea.
Bar = 1 mm. D, GUS staining of pollen
grains. Bar = 10 mm.

File:Image5.png - Revision history

Tracy: Figure 5. Expression pattern of OsGT1. A, Quantitative RT-PCR analyses in various organs. Transcript levels were normalized to UBIQUITIN and calculated by the comparative cycle threshold method. Error bars indicate SD. 7DR, Seven-day-old seedling roots; 7