IC4R004-Proteomic-2007-17849412 - Revision history

Liman: /* Research Findings */

2016-07-18T05:04:19Z

‎Research Findings

Liman: /* Research Findings */

2016-07-18T05:00:31Z

‎Research Findings

Liman: /* Research Findings */

2016-07-18T03:28:03Z

‎Research Findings

Liman: /* Protein Extraction and 2-D PAGE */

2016-07-18T03:11:03Z

‎Protein Extraction and 2-D PAGE

Liman: /* The Background of This Project */

2016-07-18T03:03:17Z

‎The Background of This Project

Liman: /* Plant Culture & Treatment */

2016-07-18T02:59:45Z

‎Plant Culture & Treatment

Liman: Created page with "==Project Title== * '''Proteomic analysis of rice (Oryza sativa) seeds during germination''' ==The Background of This Project== ==Plant Culture & Treatment== ==Protein Ex..."

2016-07-18T02:57:39Z

Created page with "==Project Title== * '''Proteomic analysis of rice (Oryza sativa) seeds during germination''' ==The Background of This Project== ==Plant Culture & Treatment== ==Protein Ex..."

New page

==Project Title==
* '''Proteomic analysis of rice (Oryza sativa) seeds during germination'''

==The Background of This Project==

==Plant Culture & Treatment==

==Protein Extraction and 2-D PAGE==

==Research Findings==

==Labs working on this Project==
* Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany,
Chinese Academy of Sciences, Beijing, P. R. China
* College of Life Sciences, Capital Normal University, Beijing, P. R. China
* Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China

==Corresponding Author==
* shshen@ibcas.ac.cn

@@ Line 16: / Line 16: @@
 * Rice seed weights increased rapidly during the first 20 h imbibition (phase I), and then experienced a stable stage until 50 h (phase II). After these two periods, the seed weights continued to increase (phase III) (Fig. 1).
-[[File:IC4R004-Proteomic-2007-17849412-f1.png |center |thumb |800px |'''Figure 1 Changes of the weight for 1000 rice seeds in the germination process.''']]
+[[File:IC4R004-Proteomic-2007-17849412-f1.png |center |thumb |500px |'''Figure 1 Changes of the weight for 1000 rice seeds in the germination process.''']]
 * 148 protein spots changed in abundance more than two-fold during germination. Among them, 63 decreased, 69 increased (including 20 induced proteins that were absent in the control gels), and another 16 increased first and then decreased (Fig. 2).
-[[File:IC4R004-Proteomic-2007-17849412-f2.png |center |thumb |800px |'''Figure 2 2-DE maps of rice seeds in the germination process. Arrows indicate the changed proteins. D, stands for the downregulated protein; M, stands for the complicated changed protein; I, stands for the induced protein which does not exist in the control gels; and U, stands for the up-regulated protein.''']]
+[[File:IC4R004-Proteomic-2007-17849412-f2.png |center |thumb |1000px |'''Figure 2 2-DE maps of rice seeds in the germination process. Arrows indicate the changed proteins. D, stands for the downregulated protein; M, stands for the complicated changed protein; I, stands for the induced protein which does not exist in the control gels; and U, stands for the up-regulated protein.''']]
 * The down-regulated proteins were mainly storage proteins, such as globulin and glutelin, and proteins associated with seed maturation, such as “early embryogenesis protein” and “late embryogenesis abundant protein”, and proteins related to desiccation, such as “abscisic acid-induced protein” and “cold regulated protein”.

@@ Line 14: / Line 14: @@
 ==Research Findings==
-* 148 protein spots changed in abundance more than two-fold during germination. Among them, 63 decreased, 69 increased (including 20 induced proteins that were absent in the control gels), and another 16 increased first and then decreased (Fig. 1).
+* Rice seed weights increased rapidly during the first 20 h imbibition (phase I), and then experienced a stable stage until 50 h (phase II). After these two periods, the seed weights continued to increase (phase III) (Fig. 1).
-[[File:IC4R004-Proteomic-2007-17849412-f1.png |center |thumb |800px |'''Figure 1 2-DE maps of rice seeds in the germination process. Arrows indicate the changed proteins. D, stands for the downregulated protein; M, stands for the complicated changed protein; I, stands for the induced protein which does not exist in the control gels; and U, stands for the up-regulated protein.''']]
+[[File:IC4R004-Proteomic-2007-17849412-f1.png |center |thumb |800px |'''Figure 1 Changes of the weight for 1000 rice seeds in the germination process.''']]
 * The down-regulated proteins were mainly storage proteins, such as globulin and glutelin, and proteins associated with seed maturation, such as “early embryogenesis protein” and “late embryogenesis abundant protein”, and proteins related to desiccation, such as “abscisic acid-induced protein” and “cold regulated protein”.

← Older revision		Revision as of 03:28, 18 July 2016
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	==Research Findings==		==Research Findings==
		+	* 148 protein spots changed in abundance more than two-fold during germination. Among them, 63 decreased, 69 increased (including 20 induced proteins that were absent in the control gels), and another 16 increased first and then decreased (Fig. 1).

		+	[[File:IC4R004-Proteomic-2007-17849412-f1.png \|center \|thumb \|800px \|'''Figure 1 2-DE maps of rice seeds in the germination process. Arrows indicate the changed proteins. D, stands for the downregulated protein; M, stands for the complicated changed protein; I, stands for the induced protein which does not exist in the control gels; and U, stands for the up-regulated protein.''']]
		+
		+	* The down-regulated proteins were mainly storage proteins, such as globulin and glutelin, and proteins associated with seed maturation, such as “early embryogenesis protein” and “late embryogenesis abundant protein”, and proteins related to desiccation, such as “abscisic acid-induced protein” and “cold regulated protein”.
		+
		+	* The degradation of storage proteins mainly happened at the late stage of germination phase II (48 h imbibition), while that of seed maturation and desiccation associated proteins occurred at the early stage of phase II (24 h imbibition).
		+
		+	* In addition to a-amylase, the up-regulated proteins were mainly those involved in glycolysis such as UDP-glucose dehydrogenase, fructokinase, phosphoglucomutase, and pyruvate decarboxylase.

	==Labs working on this Project==		==Labs working on this Project==

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 ==Protein Extraction and 2-D PAGE==
+* 0.25 g of seeds were ground in 2 mL of precooled homogenization buffer containing 20 mM Tris/HCl (pH 7.5), 250 mM sucrose, 10 mM ethylene glycol-bis(b-aminoethylether)-N,N,N0,N0- tetraacetic acid (EGTA), 1 mM PMSF, 1 mM DTT, and 1% Triton X-100. After homogenization, the homogenate was shifted into a microtube and centrifuged at 15 000 g for 15 min at 47C. The pellet was discarded, and the supernatant was mixed with 1/4 volume 50% cold trichloroacetic acid (TCA) and kept in an ice bath for 30 min. Then it was centrifuged at 15 000 g for 15 min at 47C, and the supernatant was discarded. The pellet was washed with cold acetone three times. After centrifugation, the pellet was vacuum-dried. The dried powder was solubilized in a sample buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 2% ampholine (pH 3.5–10), and 20 mM DTT. The protein solution could be used for 2-DE or stored in 2807C until used.
 ==Research Findings==

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 ==The Background of This Project==
+* Although seed germination is a major subject in plant physiological research, there is still a long way to go to elucidate the mechanism of seed germination. Recently, functional genomic strategies have been applied to study the germination of plant seeds. In this project, the researchers conducted a proteomic analysis of seed germination in rice (Oryza sativa indica cv. 9311) – a model monocot.
 ==Plant Culture & Treatment==