IC4R010-Proteomic-2004-14730683 - Revision history

Guangyi: /* Research Findings */

2016-07-17T13:41:07Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:39:48Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:38:08Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:37:06Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:35:04Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:32:21Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:21:50Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:04:16Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T13:03:12Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-17T12:52:14Z

‎Research Findings

@@ Line 35: / Line 35: @@
 *Several different patterns of change were observed,including a gradual increase in relative protein abundance occurring during RYMV infection (a total of 10 spots and 16 spots for IR64 and Azucena, respectively), a gradual decrease in relative protein abundance occurring during the RYMV infection (a total of 2 and 5 spots for IR64 and Azucena, respectively), a transient increase in relative protein abundance occurring at one or two time-points followed by a decrease (a total of 16 and 2 spots for IR64 and Azucena, respectively), a transient decrease in relative protein abundance occurring at one or two time points followed by an increase (a total of 12 and 1 spots for IR64 and Azucena, respectively). The overall patterns of differential expression are compared in Fig. 3.
 <br>
-[[File:IC4R010-Proteomic-2004-14730683-f3.png |center |thumb |10000px |'''''']]
+[[File:IC4R010-Proteomic-2004-14730683-f3.png |center |thumb |10000px |''''''Figure 3. Number of proteins with deregulated level
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@@ Line 31: / Line 31: @@
 *Three metabolic proteins showing a differential expression pattern during RYMV infection in IR64 cells were involved in glycolysis pathway (Table 1).The relative abundance of salt stress-induced protein,pathogenesis-related protein, dehydrin, chaperonins,HSPs, and ethylene-inducible protein have been observed to change during the RYMV infection (Table 1).
 <br>
-[[File:IC4R010-Proteomic-2004-14730683-t1.png |center |thumb |10000px |'''''']]
+[[File:IC4R010-Proteomic-2004-14730683-t1.png |center |thumb |10000px |''''''Table 1. Identification of proteins demonstrating relative expression level changes during RYMV infection by MS and database searching'''''']]
 <br>
 *Several different patterns of change were observed,including a gradual increase in relative protein abundance occurring during RYMV infection (a total of 10 spots and 16 spots for IR64 and Azucena, respectively), a gradual decrease in relative protein abundance occurring during the RYMV infection (a total of 2 and 5 spots for IR64 and Azucena, respectively), a transient increase in relative protein abundance occurring at one or two time-points followed by a decrease (a total of 16 and 2 spots for IR64 and Azucena, respectively), a transient decrease in relative protein abundance occurring at one or two time points followed by an increase (a total of 12 and 1 spots for IR64 and Azucena, respectively). The overall patterns of differential expression are compared in Fig. 3.

@@ Line 25: / Line 25: @@
 *Three proteins involved in metabolism revealed a differential profile for IR64 cellular suspensions under RYMV stress: relative abundance of both aldolase C1 (Fig. 2A,No. 44) and phosphoglycerate dehydrogenase (Fig. 2A,No. 13) increased late at RYMV infection, at 5 dpi and 7 dpi, respectively, whereas the relative abundance of 2,3-biphosphoglycerate-independent phosphoglycerate mutase was altered rapidly after RYMV inoculation(Fig. 2A, Nos. 14 and 15). This protein was present on 2-DE gels as doublet spots with a similar molecular mass and pI values. On the contrary, only one enzyme implied in metabolism revealed an altered expression level pattern in Azucena cellular suspensions under RYMV stress: the relative abundance of glyceraldehyde-3-phosphate dehydrogenase (Fig. 2B, No. 65) increased at 7 dpi. This identified protein spot has a molecular mass different to the theoretical mass, suggesting that it corresponded to a fragment of the protein.
 <br>
-[[File:IC4R010-Proteomic-2004-14730683-f2a.png |center |thumb |10000px |'''''']]
+[[File:IC4R010-Proteomic-2004-14730683-f2a.png |center |thumb |10000px]]
 <br>
-[[File:IC4R010-Proteomic-2004-14730683-f2b.png |center |thumb |10000px |'''''']]
+[[File:IC4R010-Proteomic-2004-14730683-f2b.png |center |thumb |10000px |''''''Figure 2. 2-DE map of (A) IR64 cellular suspension and (B) Azucena cellular suspension. The proteins were visualized by Coomassie Brilliant Blue staining. The numbers indicate proteins whose accumulation level changed under RYMV infection.'''''']]
 <br>
 *Three metabolic proteins showing a differential expression pattern during RYMV infection in IR64 cells were involved in glycolysis pathway (Table 1).The relative abundance of salt stress-induced protein,pathogenesis-related protein, dehydrin, chaperonins,HSPs, and ethylene-inducible protein have been observed to change during the RYMV infection (Table 1).

@@ Line 20: / Line 20: @@
 *In order to confirm the RYMV infection in cellular suspensions, we performed Northern blot analysis of total RNA isolated from non-stress and RYMV-inoculated cellular suspensions harvested at 1 hpi, 2 dpi, 5 dpi, and 7 dpi for both IR64 and Azucena cultivars (Fig. 1). The accumulation of newly synthesized viral RNA was followed by Northern blot analysis using a probe corresponding to RYMV ORF4. No RNA or residual RNA from virus was detected 1 hpi in both IR64 and Azucena cellular suspensions. In contrast, viral RNA (4452 nucleotides) was clearly detected after 2 dpi for both cultivars, and as previously observed in rice leaves, viral subgenomic RNAs were also detected in cellular suspensions for both cultivars.
 <br>
-[[File:IC4R010-Proteomic-2004-14730683-f1.png |center |thumb |10000px |'''''']]
+[[File:IC4R010-Proteomic-2004-14730683-f1.png |center |thumb |10000px |''''''Figure 1. Detection of viral RNA. (A) The lanes contain 10 mg of total RNA from nonstressed cellular suspensions and RYMV inoculated cellular suspensions and harvested
 <br>
 *Three proteins involved in metabolism revealed a differential profile for IR64 cellular suspensions under RYMV stress: relative abundance of both aldolase C1 (Fig. 2A,No. 44) and phosphoglycerate dehydrogenase (Fig. 2A,No. 13) increased late at RYMV infection, at 5 dpi and 7 dpi, respectively, whereas the relative abundance of 2,3-biphosphoglycerate-independent phosphoglycerate mutase was altered rapidly after RYMV inoculation(Fig. 2A, Nos. 14 and 15). This protein was present on 2-DE gels as doublet spots with a similar molecular mass and pI values. On the contrary, only one enzyme implied in metabolism revealed an altered expression level pattern in Azucena cellular suspensions under RYMV stress: the relative abundance of glyceraldehyde-3-phosphate dehydrogenase (Fig. 2B, No. 65) increased at 7 dpi. This identified protein spot has a molecular mass different to the theoretical mass, suggesting that it corresponded to a fragment of the protein.

@@ Line 26: / Line 26: @@
 [[File:IC4R010-Proteomic-2004-14730683-f2a.png |center |thumb |10000px |'''''']]
 <br>
-[[File:IC4R010-Proteomic-2004-14730683.png |center |thumb |10000px |'''''']]
+[[File:IC4R010-Proteomic-2004-14730683-f2b.png |center |thumb |10000px |'''''']]
 <br>
 *Three metabolic proteins showing a differential expression pattern during RYMV infection in IR64 cells were involved in glycolysis pathway (Table 1).The relative abundance of salt stress-induced protein,pathogenesis-related protein, dehydrin, chaperonins,HSPs, and ethylene-inducible protein have been observed to change during the RYMV infection (Table 1).
 <br>
 *Several different patterns of change were observed,including a gradual increase in relative protein abundance occurring during RYMV infection (a total of 10 spots and 16 spots for IR64 and Azucena, respectively), a gradual decrease in relative protein abundance occurring during the RYMV infection (a total of 2 and 5 spots for IR64 and Azucena, respectively), a transient increase in relative protein abundance occurring at one or two time-points followed by a decrease (a total of 16 and 2 spots for IR64 and Azucena, respectively), a transient decrease in relative protein abundance occurring at one or two time points followed by an increase (a total of 12 and 1 spots for IR64 and Azucena, respectively). The overall patterns of differential expression are compared in Fig. 3.
 ==Labs working on this Project==