IC4R012-Proteomic-2009-19201764 - Revision history

Guangyi: /* Research Findings */

2016-07-16T06:36:29Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-16T06:32:49Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-16T06:31:51Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-16T06:30:00Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-16T06:26:54Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-16T06:25:44Z

‎Research Findings

Guangyi at 06:25, 16 July 2016

2016-07-16T06:25:01Z

Guangyi: /* Research Findings */

2016-07-16T06:13:26Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-16T06:07:09Z

‎Research Findings

Guangyi: /* Research Findings */

2016-07-16T06:02:47Z

‎Research Findings

@@ Line 22: / Line 22: @@
 *As the amount of Cu in the incubation medium increased,the Cu content in the rice embryos also increased (Fig. 2A).The maximum Cu content observed in the embryos was 0.96 mg g21 (dry weight), which occurred when the seeds germinated in the presence of 200 mM Cu.
 <br>
-[[File:IC4R012-Proteomic-2009-19201764-f2a.png |center |thumb |10000px |''''''F I G. 2. Effect of Cu treatment on the content of (A) Cu.'''''']]
+[[File:IC4R012-Proteomic-2009-19201764-f2a.png |center |thumb |10000px |''''''F I G. 2. Effect of Cu treatment on the content of (A) Cu in germinating rice embryos.'''''']]
 <br>
 *The soluble protein concentration and protein thiol content in the germinating rice seed embryos were determined on the sixth day after Cu treatment. At 100 or 200 mM, but not at 50 mM, Cu significantly increased the soluble protein concentration, as compared with the control (Fig. 2B). The protein thiol content was significantly higher in the embryos treated with 200 mM Cu than in the control (Fig. 2C)
 <br>
-[[File:IC4R012-Proteomic-2009-19201764-f2b.png |center |thumb |10000px |'''''']]
+[[File:IC4R012-Proteomic-2009-19201764-f2b.png |center |thumb |10000px |''''''Effect of Cu treatment on the content of(B) total protein in germinating rice embryos. Rice seeds were treated with the indicated concentrations of Cu for 6 d. Values shown represent means+ s.e. (n ¼ 3) for three different experiments. Means denoted by the same letter did not differ significantly (P � 0.05 according to Duncan’s multiple range test).'''''']]
 <br>
-[[File:IC4R012-Proteomic-2009-19201764-f2c.png |center |thumb |10000px |'''''']]
+[[File:IC4R012-Proteomic-2009-19201764-f2c.png |center |thumb |10000px |''''''Effect of Cu treatment on the content of(C) protein thiol (PT) in germinating rice embryos. Rice seeds were treated with the indicated concentrations of Cu for 6 d. Values shown represent means+ s.e. (n ¼ 3) for three different experiments. Means denoted by the same letter did not differ significantly (P � 0.05 according to Duncan’s multiple range test).'''''']]
 <br>
 *As shown in Fig. 3, SDS–PAGE of the total proteins from germinating rice embryos yielded some visible differences in the protein band patterns of the control versus Cu-treated embryos. These differences became more pronounced when the extracts were first heated at 808C for 5 min before loading. The intensities of two protein bands of about 12 kDa and 20 kDa increased as the concentration of Cu in the incubation medium increased.

@@ Line 22: / Line 22: @@
 *As the amount of Cu in the incubation medium increased,the Cu content in the rice embryos also increased (Fig. 2A).The maximum Cu content observed in the embryos was 0.96 mg g21 (dry weight), which occurred when the seeds germinated in the presence of 200 mM Cu.
 <br>
-[[File:IC4R012-Proteomic-2009-19201764-f2a.png |center |thumb |10000px |'''''']]
+[[File:IC4R012-Proteomic-2009-19201764-f2a.png |center |thumb |10000px |''''''F I G. 2. Effect of Cu treatment on the content of (A) Cu.'''''']]
 <br>
 *The soluble protein concentration and protein thiol content in the germinating rice seed embryos were determined on the sixth day after Cu treatment. At 100 or 200 mM, but not at 50 mM, Cu significantly increased the soluble protein concentration, as compared with the control (Fig. 2B). The protein thiol content was significantly higher in the embryos treated with 200 mM Cu than in the control (Fig. 2C)

@@ Line 32: / Line 32: @@
 *As shown in Fig. 3, SDS–PAGE of the total proteins from germinating rice embryos yielded some visible differences in the protein band patterns of the control versus Cu-treated embryos. These differences became more pronounced when the extracts were first heated at 808C for 5 min before loading. The intensities of two protein bands of about 12 kDa and 20 kDa increased as the concentration of Cu in the incubation medium increased.
 <br>
-[[File:IC4R012-Proteomic-2009-19201764-f3.png |center |thumb |10000px |'''''']]
+[[File:IC4R012-Proteomic-2009-19201764-f3.png |center |thumb |10000px |''''''F I G. 3. SDS–PAGE of germinating rice embryo extracts from seeds treated with the indicated concentrations of Cu for 6 d. Equal amounts of protein(20 mg) were loaded in each gel lane. Arrows indicate protein bands altered by Cu treatment. Experiments were performed three times with similar results.]]
 <br>
 *The change in protein patterns was further analysed by 2-DE for better separation and characterization. Digital image analysis of a Coomassie-stained 2-DE gel revealed .520 rice embryo protein spots, of which 16 of the smaller proteins(5–25 kDa) were Cu-responsive. Compared with the control,Cu treatment up-regulated expression of 13 spots and downregulated expression of three spots (Fig. 4).

@@ Line 36: / Line 36: @@
 *The change in protein patterns was further analysed by 2-DE for better separation and characterization. Digital image analysis of a Coomassie-stained 2-DE gel revealed .520 rice embryo protein spots, of which 16 of the smaller proteins(5–25 kDa) were Cu-responsive. Compared with the control,Cu treatment up-regulated expression of 13 spots and downregulated expression of three spots (Fig. 4).
 <br>
-[[File:IC4R012-Proteomic-2009-19201764-f4a.png |center |thumb |10000px |'''''']]
+[[File:IC4R012-Proteomic-2009-19201764-f4a.png |center |thumb |10000px ]]
-[[File:IC4R012-Proteomic-2009-19201764-f4b.png |center |thumb |10000px |'''''']]
+[[File:IC4R012-Proteomic-2009-19201764-f4b.png |center |thumb |10000px |''''''F I G. 4. Two-dimensional PAGE of germinating rice embryo extracts from seeds treated with 0 (Con) or 200 mM Cu for 6 d. Equal amounts of protein(1 mg) were loaded on each gel. Arrows indicate the proteins identified.'''''']]
 <br>

@@ Line 31: / Line 31: @@
 <br>
 *As shown in Fig. 3, SDS–PAGE of the total proteins from germinating rice embryos yielded some visible differences in the protein band patterns of the control versus Cu-treated embryos. These differences became more pronounced when the extracts were first heated at 808C for 5 min before loading. The intensities of two protein bands of about 12 kDa and 20 kDa increased as the concentration of Cu in the incubation medium increased.
 <br>
 *The change in protein patterns was further analysed by 2-DE for better separation and characterization. Digital image analysis of a Coomassie-stained 2-DE gel revealed .520 rice embryo protein spots, of which 16 of the smaller proteins(5–25 kDa) were Cu-responsive. Compared with the control,Cu treatment up-regulated expression of 13 spots and downregulated expression of three spots (Fig. 4).
 <br>

@@ Line 25: / Line 25: @@
 <br>
 *The soluble protein concentration and protein thiol content in the germinating rice seed embryos were determined on the sixth day after Cu treatment. At 100 or 200 mM, but not at 50 mM, Cu significantly increased the soluble protein concentration, as compared with the control (Fig. 2B). The protein thiol content was significantly higher in the embryos treated with 200 mM Cu than in the control (Fig. 2C)
 ==Labs working on this Project==

@@ Line 20: / Line 20: @@
 [[File:IC4R012-Proteomic-2009-19201764-f1.png |center |thumb |10000px |''''''F I G. 1. Effect of Cu on rice radicle elongation. Rice seeds were treated with the indicated concentrations of Cu for 4, 6 or 8 d. Values shown represent means+ s.e. (n ¼ 3) for three different experiments. Means denoted by the same letter did not differ significantly (P � 0.05 according to Duncan’s multiple range test).'''''']]
 <br>
 ==Labs working on this Project==

@@ Line 16: / Line 16: @@
 ==Research Findings==
-*As compared with the control (no Cu; Fig. 1), treatment with 50 or 100 mM Cu for 4 d had no significant effect on radicle
+*As compared with the control (no Cu; Fig. 1), treatment with 50 or 100 mM Cu for 4 d had no significant effect on radicle elongation. On the other hand, prolonged treatment (6 or 8 d) with Cu (�50 mM) had a negative effect, with radicle length decreasing as the concentration of Cu in the incubation medium increased.
 <br>
 [[File:IC4R012-Proteomic-2009-19201764-f1.png |center |thumb |10000px |''''''F I G. 1. Effect of Cu on rice radicle elongation. Rice seeds were treated with the indicated concentrations of Cu for 4, 6 or 8 d. Values shown represent means+ s.e. (n ¼ 3) for three different experiments. Means denoted by the same letter did not differ significantly (P � 0.05 according to Duncan’s multiple range test).'''''']]