OrfB - Revision history

192.168.72.52 at 05:34, 15 May 2015

2015-05-15T05:34:34Z

Hui Sun: /* Labs working on this gene */

2014-06-07T15:28:57Z

‎Labs working on this gene

Hui Sun: /* Labs working on this gene */

2014-06-07T15:28:00Z

‎Labs working on this gene

Hui Sun: /* Expression */

2014-06-07T15:18:52Z

‎Expression

Hui Sun: /* References */

2014-06-07T15:17:49Z

‎References

Hui Sun: /* Expression */

2014-06-07T15:16:36Z

‎Expression

Hui Sun: /* Knowledge Extension */

2014-06-07T15:06:32Z

‎Knowledge Extension

Hui Sun: /* Expression */

2014-06-07T15:03:52Z

‎Expression

Hui Sun: /* Mutation */

2014-06-07T15:00:26Z

‎Mutation

Hui Sun: /* Function */

2014-06-07T14:59:58Z

‎Function

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-It is generally believed that the CMS trait is
+Please input function information here.
-===Mutation===
+===Expression===
-Experimental evidence for polymorphisms in orfB structural organization and mitochondrial transcript profiles of the orfB gene in the CMS-WA rice system. The sterile line orfB gene transcript profile was characterized by two transcripts of ~1.1 kb and ~0.7 kb, and one ~0.7 kb transcript was detected in the fertile lines. The ~1.1 kb transcript present in the sterile line remained unedited. However, in the presence of nuclear encoded restoration of fertility (Rf) gene(s) in fertile restored hybrid lines (APMS-6A × BR-1870; F1 generation), the ~1.1 kb orfB transcripts were fully edited. The editing of the orfB gene ~1.1 kb transcript co-segregated with fertility restoring alleles in a segregating population of F2 progeny of restored hybrid F1 plants.<ref name="ref4" />
+Please input expression information here.
-===Expression===
+===Evolution===
-OrfB gene is located at the
+Please input evolution information here.
-=== Evolution ===
+You can also add sub-section(s) at will.
 ==Labs working on this gene==
-*Advanced Laboratory for Plant Genetic Engineering (formerly IIT-BREF Biotek), Indian Institute of Technology, Kharagpur- 721302, India
+Please input related labs here.
 ==References==
-<references>
+==Structured Information==
-<ref name="ref1"> Schnable PS, Wise RP (1998) The molecular basis of cytoplasmic
+     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Mitochondrion]]

@@ Line 35: / Line 35: @@
 ==Labs working on this gene==
-*Advanced Laboratory for Plant Genetic Engineering (formerly IIT-BREF
+*Advanced Laboratory for Plant Genetic Engineering (formerly IIT-BREF Biotek), Indian Institute of Technology, Kharagpur- 721302, India
 ==References==

@@ Line 35: / Line 35: @@
 ==Labs working on this gene==
-Please input related labs here.
+*Advanced Laboratory for Plant Genetic Engineering (formerly IIT-BREF
 ==References==

@@ Line 26: / Line 26: @@
 sequences were caused by rearrangements of mitochondrial genomes resulting in the birth of new orfs composed of
 fragments of other mitochondrial respiratory-related genes
-or non-coding sequences <ref name="ref5" /><ref name="ref6" /><ref name="ref7" /> . Usually, such
+or non-coding sequences <ref name="ref1" /><ref name="ref3" /><ref name="ref5" /> . Usually, such
 chimeric orfs were located at the flanking of mitochondrial
 respiratory/ATP synthesis-related genes and co-transcribed

@@ Line 49: / Line 49: @@
 <ref name="ref4">Srirupa Das, et al.An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of
 Oryza sativa L. subsp. indica.BMC Plant Biology 2010, 10:39 </ref>
 {{Mitochondrion|
 GeneName = orfB|

← Older revision		Revision as of 15:16, 7 June 2014
Line 20:		Line 20:

	===Expression===		===Expression===
		+	OrfB gene is located at the
		+	downstream of mitochondrial nad2 gene in the CMS.The
		+	novel orfB gene had high similarity with YMF19 family
		+	protein.In many cases, CMS-associated genes or
		+	sequences were caused by rearrangements of mitochondrial genomes resulting in the birth of new orfs composed of
		+	fragments of other mitochondrial respiratory-related genes
		+	or non-coding sequences <ref name="ref5" /><ref name="ref6" /><ref name="ref7" /> . Usually, such
		+	chimeric orfs were located at the flanking of mitochondrial
		+	respiratory/ATP synthesis-related genes and co-transcribed
		+	with these genes.

	=== Evolution ===		=== Evolution ===

← Older revision		Revision as of 15:03, 7 June 2014
Line 20:		Line 20:

	===Expression===		===Expression===
−	~~Editing of the orfB transcripts~~
−	~~(a) The fertile line~~
−	~~Mitochondrial RNA editing of the orfB transcript was~~
−	~~assessed in the fertile rice line. Fourteen cDNA clones~~
−	~~obtained from cDNA library of fertile rice line were~~
−	~~sequenced. Determination of the orfB cDNA sequence~~
−	~~from overlapping clones from the cDNA library showed~~
−	~~four C→T conversions within the coding region relative~~
−	~~to the orfB genomic sequence.~~

	=== Evolution ===		=== Evolution ===

@@ Line 10: / Line 10: @@
 ===Mutation===
-Experimental evidence for polymorphisms in orfB structural organization and mitochondrial transcript profiles of the orfB gene in the CMS-WA rice system. The sterile line orfB gene transcript profile was characterized by two transcripts of ~1.1 kb and ~0.7 kb, and one ~0.7 kb transcript was detected in the fertile lines. The ~1.1 kb transcript present in the sterile line remained unedited. However, in the presence of nuclear encoded restoration of fertility (Rf) gene(s) in fertile restored hybrid lines (APMS-6A × BR-1870; F1 generation), the ~1.1 kb orfB transcripts were fully edited. The editing of the orfB gene ~1.1 kb transcript co-segregated with fertility restoring alleles in a segregating population of F2 progeny of restored hybrid F1 plants.<ref name="ref1" />
+Experimental evidence for polymorphisms in orfB structural organization and mitochondrial transcript profiles of the orfB gene in the CMS-WA rice system. The sterile line orfB gene transcript profile was characterized by two transcripts of ~1.1 kb and ~0.7 kb, and one ~0.7 kb transcript was detected in the fertile lines. The ~1.1 kb transcript present in the sterile line remained unedited. However, in the presence of nuclear encoded restoration of fertility (Rf) gene(s) in fertile restored hybrid lines (APMS-6A × BR-1870; F1 generation), the ~1.1 kb orfB transcripts were fully edited. The editing of the orfB gene ~1.1 kb transcript co-segregated with fertility restoring alleles in a segregating population of F2 progeny of restored hybrid F1 plants.<ref name="ref4" />
 RFLPs were observed for the orfB gene in the mitochondrial genome between the sterile and the fertile lines,showing that mitochondrial orfB gene was present as a single copy with differential organization in the sterile and fertile lines.
 Mitochondrial RNA editing of the orfB transcript was assessed in the fertile rice line. Fourteen cDNA clones obtained from cDNA library of fertile rice line were

@@ Line 7: / Line 7: @@
 which result from the expressions of chimeric open
 reading frames (orf) or alterations in genomic structure and
-transcriptions patterns of mitochondrial genes <ref name="ref1,2,3" />.
+transcriptions patterns of mitochondrial genes <ref name="ref1" /><ref name="ref2" /><ref name="ref3" />.
 ===Mutation===