Os01g0201700 - Revision history

Xysj2012: /* Structured Information */

2015-05-13T07:03:47Z

‎Structured Information

Ban Buzhu at 01:50, 24 May 2014

2014-05-24T01:50:02Z

Ban Buzhu at 01:48, 24 May 2014

2014-05-24T01:48:41Z

Ban Buzhu at 16:49, 22 May 2014

2014-05-22T16:49:01Z

Ban Buzhu at 16:47, 22 May 2014

2014-05-22T16:47:10Z

Ban Buzhu at 16:26, 22 May 2014

2014-05-22T16:26:52Z

Ban Buzhu at 16:25, 22 May 2014

2014-05-22T16:25:28Z

Ban Buzhu at 16:23, 22 May 2014

2014-05-22T16:23:02Z

Ban Buzhu at 16:19, 22 May 2014

2014-05-22T16:19:55Z

Ban Buzhu at 16:17, 22 May 2014

2014-05-22T16:17:14Z

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 </references>
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Knowledge Extension===
 *[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]
- * the ABC model can also partially explain how stamen determination is specified in the monocot plant rice <ref name="ref2" />. For example, a rice B-class gene, SUPERWOMEN1 (SPW1 or MADS16), which is orthologous to the Arabidopsis APETALA3 gene, has been shown to be crucial for stamen specification . spw1 mutants show homeotic conversions of stamens to carpels and lodicules to palea/lemma-like structures. In Arabidopsis, the C-class gene AGAMOUS (AG) acts to specify stamen and carpel identities and floral meristem determinacy . Studies in rice identified two C-class MADS box genes, MADS3 and MADS58, that may have distinct functions in specifying stamen identity, with MADS3 playing a more important role <ref name="ref2" />.
+*the ABC model can also partially explain how stamen determination is specified in the monocot plant rice <ref name="ref2" />. For example, a rice B-class gene, SUPERWOMEN1 (SPW1 or MADS16), which is orthologous to the Arabidopsis APETALA3 gene, has been shown to be crucial for stamen specification . spw1 mutants show homeotic conversions of stamens to carpels and lodicules to palea/lemma-like structures. In Arabidopsis, the C-class gene AGAMOUS (AG) acts to specify stamen and carpel identities and floral meristem determinacy . Studies in rice identified two C-class MADS box genes, MADS3 and MADS58, that may have distinct functions in specifying stamen identity, with MADS3 playing a more important role <ref name="ref2" />.
 *Other MADS box genes, such as SPOROCYTELESS/NOZZLE (SPL/NZZ) <ref name="ref5" /><ref name="ref6" />.andAG from Arabidopsis and MADS2 from maize <ref name="ref6" />, have been implicated in regulating anther development. SPL/NZZ regulates the formation of anther walls and pollen mother cells, as the primary sporogenous cells cannot form pollen mother cells in spl anthers, thereby blocking early cell differentiation [5].AGhas been shown to activate the expression of SPL/NZZ, suggesting that this gene is necessary for early stamen development <ref name="ref7" />. During later developmental stages, AG continues its expression in the anther and regulates anther dehiscence by directly regulating the expression of the gene that encodes a jasmonic acid (JA) synthetic enzyme, DEFECTIVE IN ANTHER DEHISCENCE1 <ref name="ref7" />. Maize MADS2 is required for anther dehiscence and pollen maturation, and knockdown of MADS2 resulted in abortion of anthers and defective pollen development<ref name="ref7" />..

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 ===Knowledge Extension===
-[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]
+*[http://ricewiki.big.ac.cn/index.php?title=Os02g0170300 OsMADS29 1.5 Knowledge Extension ]
 ==Labs working on this gene==
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 <ref name="ref4">
 Moon, Y.H., Kang, H.G., Jung, J.Y., Jeon, J.S., Sung, S.K., and An, G. (1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiol. 120: 1193–1204</ref>
 </references>

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 [[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]
 [[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]
 '''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />.

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 [[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]
 [[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]
 '''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />.

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 *mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.
 * mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />.
-*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.
+*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable  '''OsMADS3''' transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />.
 * mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.

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 ===Mutation===
 '''OsMADS3''' alleles:mads3-1,mads3-2,mads3-3,mads3-4.<br>
-*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs, whereas mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />.
+*mads3-1 is a weak allele carrying an insertion in the C-terminal region of the gene and showing no defective floral organs<ref name="ref2" />.
-*mads3-3 is a strong allele in the Dongjin (a japonica cultivar) background. It contains a T-DNA insertion in the second intron of the gene, which leads to no detectable MADS3 transcript and homeotic transformation of nearly all stamens in whorl 3 into lodicule-like organs <ref name="ref2" />. mads3-4, which results in a mutation in the middle region of the K-domain of '''OsMADS3''', seems to be another intermediate mutant with reduced expression of '''OsMADS3'''<ref name="ref1" />.
+* mads3-2 is an intermediate allele with a mutation at the C terminus of the K-domain and reduced expression of the gene, which resulted in mild transformation of stamens into lodicules<ref name="ref2" />.
 ===Expression===

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 [[File:Figure 1. Phenotypic Analysis of mads3-4..png|right|thumb|320px|'''Figure 1.''' ''Mutant VS. WT(from reference) <ref name="ref1" />.<br>(A) Plant after bolting.(B) Panicle at the heading stage.(C) to (F) Flowers at stage 11 ([C] and [E]) and stage 12 ([D] and [F]) .(G) and (H) Pollen grains at stage 12 stained by I2-KI.(I) and (J) Transverse sections of fresh anthers prepared from flowers like those in (D) and (F) without the staining treatment.
 MP, mature pollen; Msp, microspore; T, tapetum; WT, wild type. Bars = 5 mm in (C) to (F), 50 mm in (G) and (H), and 10 mm in (I) and (J).'']]
-[[File:Figure 2. Analyses of ROS and Superoxide Anion levels in Wild-Type.png|right|thumb|320px|'''Figure 2.''' ''Analyses of ROS and Superoxide Anion levels in Wild-Type and mads3-4 Anthers. <ref name="ref2" />.<br>A) and (B) ROS (A) and superoxide anion (O2; [B]) levels. Data are presented as means 6 SE (n = 3).(C) and (D) NBT staining analysis of O2 production from early stages 8 to 12 showing the shallow to dark-blue colors.(E) and (F) O2 localization in the tapetum and microspores in tissue sections of anthers at stage 9. Bars = 5 mm. Msp, microspore; T, tapetum; WT, wild type. BS8, anthers before stage 8; S8-S12, anthers at stage 8 to 12.'']]
-[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 3.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]
+[[File:Figure 3. Analyses of the MT-1-4b-amiRNA Lines.png|right|thumb|320px|'''Figure 2.''' ''Analyses of the MT-1-4b-amiRNA Lines. <ref name="ref1" />.'']]
-[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 4.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]
+[[File:Figure 4. Molecular Identification of Rice MADS3..png|right|thumb|320px|'''Figure 3.''' ''Molecular Identification of Rice MADS3. <ref name="ref1" />'']]
-[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 5.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]
+[[File:Figure 5. Rice MADS3 Expression Pattern Analysis..png|right|thumb|320px|'''Figure 4.''' Rice MADS3 Expression Pattern Analysis. <ref name="ref1" />'']]
-[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 6.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]
+[[File:Figure 6. Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily.png|right|thumb|320px|'''Figure 5.''' Phylogenetic Analysis of MADS Domain Proteins in the AG Subfamily. <ref name="ref2" />'']]
 '''OsMADS3'''(Os01g0201700)plays key roles in both stamen identity specification and late anther development ,and Loss-of-function mutants of '''OsMADS3''' result severe defects in stamen identity and lodicule number<ref name="ref1" /><ref name="ref2" />.'''OsMADS3''' is highly expressed in the tapetum and microspores during late anther development and is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b<ref name="ref1" />.