Os01g0323600 - Revision history

Guangyi: /* Expression */

2016-08-08T09:21:05Z

‎Expression

Guangyi: /* Expression */

2016-08-08T09:19:56Z

‎Expression

Guangyi at 09:16, 8 August 2016

2016-08-08T09:16:36Z

Guangyi: /* Function */

2016-08-08T09:16:00Z

‎Function

Guangyi: /* Expression */

2016-08-08T09:09:03Z

‎Expression

Guangyi: /* Expression */

2016-08-08T08:54:33Z

‎Expression

Guangyi: /* Expression */

2016-08-08T08:47:09Z

‎Expression

Guangyi: /* Expression */

2016-08-08T08:38:13Z

‎Expression

Guangyi: /* References */

2016-08-08T03:43:46Z

‎References

Guangyi: /* References */

2016-08-08T03:43:21Z

‎References

@@ Line 12: / Line 12: @@
 used as WT yeast, AtSAM1 was used as a positive control.'''''']]
 *Real-time RT-PCR analysis of OsSAMS1 revealed reduced expression in the transgenic lines W, M and S, by 60.4%, 4.8%, 2.6% that of the WT, respectively, and OsSAMS2 and OsSAMS3 also showed a significant decrease in expression levels (Fig. 3A). The expression of OsSAMS2 was decreased to 76.1%, 31.5% and 15.9% in the W, M and S lines.
-[[File:15-f3.png |center |thumb |10000px |'''''']]
+[[File:15-f3.png |center |thumb |10000px |''''''Fig. 3. Identification of OsSAMS1-RNAi transgenic plants. (A) Real-time RT-PCR analysis of the expression of OsSAMS1, 2, 3 genes in OsSAMS1-RNAi transgenic seedlings. Error bars represent ±SD.'''''']]
 <br>

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 ===Expression===
 *OsSAMS1 was expressed abundantly in almost every tissue. OsSAMS2 was preferentially expressed in young roots and panicles. By contrast, the expression of OsSAMS3 was weak compared with OsSAMS1 (Fig. 1A).Thus, OsSAMS genes were constitutively expressed but the various homologues may have different functions during development.The double mutant containing OsSAMS1 or 2, or 3 alone no longer depended on externally added SAM when their expression was induced in the presence of galactose (Fig. 1B). Thus, OsSAMS1, 2 and 3 were all genetically complemented for the functional SAMS enzyme in the yeast double mutant in vivo. Moreover, as shown in Fig. 1B, the OsSAMS1 activity was comparable to OsSAMS2, and OsSAMS3 activity was weak.
-[[File:15-f1.png |center |thumb |10000px |'''''']]
+[[File:15-f1.png |center |thumb |10000px |''''''Fig. 1. Biochemical characterization of Oryza sativa S-adenosylmethionine synthetase (OsSAMS1, 2, 3) and expression patterns. (A) Expression profile of OsSAMS1,2, 3 in different ZH 10 organs detected by real-time RT-PCR. Error bars represent±SD. (B) OsSAMS1, 2, 3 complemented yeast sam1 sam2 double mutant W744-1A.
 *Real-time RT-PCR analysis of OsSAMS1 revealed reduced expression in the transgenic lines W, M and S, by 60.4%, 4.8%, 2.6% that of the WT, respectively, and OsSAMS2 and OsSAMS3 also showed a significant decrease in expression levels (Fig. 3A). The expression of OsSAMS2 was decreased to 76.1%, 31.5% and 15.9% in the W, M and S lines.
 [[File:15-f3.png |center |thumb |10000px |'''''']]

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-Please input one-sentence summary here.
+OsSAMS1, 2 and 3 are essential for histone H3K4me3 and DNA methylation to regulate gene expression related to flowering in Oryza sativa.
 ==Annotated Information==
 ===Function===
@@ Line 13: / Line 12: @@
 [[File:15-f3.png |center |thumb |10000px |'''''']]
 <br>
 ==Labs working on this gene==

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-Please input function information here.
+*OsSAMS1, 2 and 3 are essential for histone H3K4me3 and DNA methylation to regulate gene expression related to flowering in Oryza sativa.
 ===Expression===

@@ Line 7: / Line 7: @@
 ===Expression===
 *OsSAMS1 was expressed abundantly in almost every tissue. OsSAMS2 was preferentially expressed in young roots and panicles. By contrast, the expression of OsSAMS3 was weak compared with OsSAMS1 (Fig. 1A).Thus, OsSAMS genes were constitutively expressed but the various homologues may have different functions during development.The double mutant containing OsSAMS1 or 2, or 3 alone no longer depended on externally added SAM when their expression was induced in the presence of galactose (Fig. 1B). Thus, OsSAMS1, 2 and 3 were all genetically complemented for the functional SAMS enzyme in the yeast double mutant in vivo. Moreover, as shown in Fig. 1B, the OsSAMS1 activity was comparable to OsSAMS2, and OsSAMS3 activity was weak.
 *Real-time RT-PCR analysis of OsSAMS1 revealed reduced expression in the transgenic lines W, M and S, by 60.4%, 4.8%, 2.6% that of the WT, respectively, and OsSAMS2 and OsSAMS3 also showed a significant decrease in expression levels (Fig. 3A). The expression of OsSAMS2 was decreased to 76.1%, 31.5% and 15.9% in the W, M and S lines.
 ===Evolution===

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 ===Expression===
-Please input expression information here.
+*OsSAMS2 was preferentially expressed in young roots and panicles.
 ===Evolution===

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 ==References==
 *'''Baurle I, Dean C.''' The timing of developmental transitions in plants. Cell 2006;125:655–64.
 ==Structured Information==
       [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]