Os01g0367100 - Revision history

Xysj2012 at 15:54, 30 June 2016

2016-06-30T15:54:26Z

Rice2012: Reverted edits by 123.113.45.72 (talk) to last revision by Xysj2012

2015-06-09T01:53:51Z

Reverted edits by 123.113.45.72 (talk) to last revision by Xysj2012

123.113.45.72: /* References */

2015-05-19T15:32:05Z

‎References

Xysj2012: /* Structured Information */

2015-05-13T07:14:11Z

‎Structured Information

Zhuo CHEN: /* References */

2014-05-07T16:19:16Z

‎References

Zhuo CHEN: /* Function */

2014-05-07T15:44:54Z

‎Function

Zhuo CHEN: /* Function */

2014-05-07T15:41:46Z

‎Function

Zhuo CHEN: /* Subcellular Localization */

2014-05-07T15:34:23Z

‎Subcellular Localization

Zhuo CHEN at 15:27, 7 May 2014

2014-05-07T15:27:40Z

Zhuo CHEN at 15:18, 7 May 2014

2014-05-07T15:18:26Z

← Older revision		Revision as of 15:54, 30 June 2016
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−	The rice ''Os01g0367100'' has been reported as ''PHD1'' in 2011. ~~''PHD1''~~ encodes a novel chloroplast-localized UDP-glucose epimerase (UGE) which plays an important role in supplying sufficient galactolipids to thylakoid membranes for proper chloroplast biogenesis and photosynthetic activity.<ref name="ref1" />	+	The rice ''Os01g0367100'' has been reported as ''PHD1'' in 2011. This gene encodes a novel chloroplast-localized UDP-glucose epimerase (UGE) which plays an important role in supplying sufficient galactolipids to thylakoid membranes for proper chloroplast biogenesis and photosynthetic activity.<ref name="ref1" />

	==Annotated Information==		==Annotated Information==

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 <references>
 <ref name="ref1">
-Li C, Wang Y, Liu L, et al. A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency[J]. PLoS genetics, 2011, 7(7): e1002196.
+Li C, Wang Y, Liu L, et al. A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency[J]. PLoS genetics, 2011, 7(7): e1002196.</ref>
 </references>

@@ Line 36: / Line 36: @@
 <references>
 <ref name="ref1">
-Li C, Wang Y, Liu L, et al. A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency[J]. PLoS genetics, 2011, 7(7): e1002196.</ref>
+Li C, Wang Y, Liu L, et al. A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency[J]. PLoS genetics, 2011, 7(7): e1002196.
 </references>

@@ Line 40: / Line 40: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 <ref name="ref1">
 Li C, Wang Y, Liu L, et al. A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis and improves photosynthetic efficiency[J]. PLoS genetics, 2011, 7(7): e1002196.</ref>
 ==Structured Information==

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 * ''PHD1'' encodes a novel chloroplast-localized UDP-glucose epimerase (UGE), which is conserved in the plant kingdom. <ref name="ref1" />
 * ''PHD1'' plays an important role in supplying sufficient galactolipids to thylakoid membranes for proper chloroplast biogenesis and photosynthetic activity and will be useful for improving crop yields and for bioenergy crop engineering.<ref name="ref1" />
-'''GO assignment(s):'''GO:0003978, GO:0019375, GO:0009507
+'''GO assignment(s):''' GO:0003978, GO:0019375, GO:0009507
 ===Wild Type VS. Mutant===

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 ===Subcellular Localization===
-* Confocal micrographs showing chloroplast targeting of PHD1(Figure 4A). Rice protoplasts transformed with 35S::PHD1-GFP (upper panel) and 35S::GFP (lower panel) plasmids are shown. Chlorophyll autofluorescence (middle); GFP fluorescence (left); merged images (right). Bars = 5 µm.  <ref name="ref1" />
+* Confocal micrographs showing chloroplast targeting of ''PHD1''(Figure 4A). Rice protoplasts transformed with ''35S::PHD1-GFP'' (upper panel) and ''35S::GFP'' (lower panel) plasmids are shown. Chlorophyll autofluorescence (middle); GFP fluorescence (left); merged images (right). Bars = 5 µm.  <ref name="ref1" />
-* PHD1 protein distribution in chloroplast subfractions. Percoll-purified intact chloroplasts were lysed and subjected to differential centrifugation fractionation into envelope, stroma, and thylakoid fractions. Proteins were separated by SDS-PAGE, and blotted against the PHD1 antibody and specific chloroplast subcompartment protein antibodies. Tic 40 is an envelope membrane protein, RbcL a stroma protein, and PsbA a thylakoid membrane protein. HSP82 was used as a cytosolic protein marker. About 15 µg of total proteins from extrachloroplast (Ep), purified chloroplast (Cp), envelope (E), stroma (S), and thylakoid (T) subfractions were loaded per line. RbcL seen in the Ep fraction is most likely due to leakage from the stroma of broken chloroplasts. (Figure 4B)<ref name="ref1" />
+* ''PHD1'' protein distribution in chloroplast subfractions. Percoll-purified intact chloroplasts were lysed and subjected to differential centrifugation fractionation into envelope, stroma, and thylakoid fractions. Proteins were separated by SDS-PAGE, and blotted against the ''PHD1'' antibody and specific chloroplast subcompartment protein antibodies. ''Tic 40'' is an envelope membrane protein, ''RbcL'' a stroma protein, and ''PsbA'' a thylakoid membrane protein. ''HSP82'' was used as a cytosolic protein marker. About 15 µg of total proteins from extrachloroplast (Ep), purified chloroplast (Cp), envelope (E), stroma (S), and thylakoid (T) subfractions were loaded per line. ''RbcL'' seen in the Ep fraction is most likely due to leakage from the stroma of broken chloroplasts. (Figure 4B)<ref name="ref1" />
-* Immunogold localization of PHD1. Thin sections of chloroplasts in leaf mesophyll cells were incubated with PHD1 antibodies (Figure 4C and Figure 4D) and preimmune serum (Figure 4E and Figure 4F). The gold label is found preferentially associated with thylakoids of the chloroplasts as seen in (Figure 4D). Chl, chloroplast; Cyt, cytosol; Mit, mitochondria; CW, cell wall. Bar = 0.5 µm.<ref name="ref1" /><br><br>
+* Immunogold localization of ''PHD1''. Thin sections of chloroplasts in leaf mesophyll cells were incubated with ''PHD1'' antibodies (Figure 4C and Figure 4D) and preimmune serum (Figure 4E and Figure 4F). The gold label is found preferentially associated with thylakoids of the chloroplasts as seen in (Figure 4D). Chl, chloroplast; Cyt, cytosol; Mit, mitochondria; CW, cell wall. Bar = 0.5 µm.<ref name="ref1" /><br><br>
 [[File:Subcellular localization of PHD1.png|center|thumb|500px|'''Figure 4.''' ''Subcellular localization of PHD1 (from reference<ref name="ref1" />).'']]

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 ===Evolution===
-* The phylogenetic analyses between ''PHD1'' and its 16 putative homologs  made by ''Chunlai Li'' et al. in 2011 showed that ''PHD1'' is closely related to ''Sb03g014730'' from sorghum and ''LOC100193101'' from maize.(Figure 3)<ref name="ref1" />
+* The phylogenetic analyses between ''PHD1'' and its 16 putative homologs  made by ''Chunlai Li'' et al. in 2011 showed that ''PHD1'' is closely related to ''Sb03g014730'' from sorghum and ''LOC100193101'' from maize.(Figure 3)<ref name="ref1" /><br><br>
 [[File:Phylogenetic_analysis_of_PHD1.png|center|thumb|500px|'''Figure 3.''' ''Phylogenetic analysis of PHD1 (from reference<ref name="ref1" />).'']]
 ==Labs working on this gene==

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 ===Expression===
-* ''in situ'' hybridization performed by ''Chunlai Li'' et al. showed that the ''PHD1'' signal was detected in the shoot apical meristem and young leaves (B), leaf mesophyll cells around vascular bundles (C), young inflorescences (D), and axillary buds (E).<ref name="ref1" /><br><br>
+* ''in situ'' hybridization performed by ''Chunlai Li'' et al. showed that the ''PHD1'' signal was detected in the shoot apical meristem and young leaves (Figure 2B), leaf mesophyll cells around vascular bundles (Figure 2C), young inflorescences (Figure 2D), and axillary buds (Figure 2E).<ref name="ref1" /><br><br>
 [[File:Expression_analysis_of_PHD1.png|center|thumb|500px|'''Figure 2.''' ''Expression analysis of PHD1 (from reference<ref name="ref1" />).'']]
 ===Evolution===
-* The phylogenetic analyses between ''PHD1'' and its 16 putative homologs  made by ''Chunlai Li'' et al. in 2011 showed that ''PHD1'' is closely related to ''Sb03g014730'' from sorghum and ''LOC100193101'' from maize.<ref name="ref1" />
+* The phylogenetic analyses between ''PHD1'' and its 16 putative homologs  made by ''Chunlai Li'' et al. in 2011 showed that ''PHD1'' is closely related to ''Sb03g014730'' from sorghum and ''LOC100193101'' from maize.(Figure 3)<ref name="ref1" />
 [[File:Phylogenetic_analysis_of_PHD1.png|center|thumb|500px|'''Figure 3.''' ''Phylogenetic analysis of PHD1 (from reference<ref name="ref1" />).'']]