Os01g0571300 - Revision history

Xysj2012: /* Structured Information */

2015-05-13T07:16:04Z

‎Structured Information

Zhangye at 15:19, 26 December 2014

2014-12-26T15:19:26Z

Zhangye: /* Expression */

2014-12-26T12:52:23Z

‎Expression

Zhangye: /* Knowledge Extension */

2014-12-26T12:49:26Z

‎Knowledge Extension

Zhangye: /* Evolution */

2014-12-26T12:48:13Z

‎Evolution

Zhangye: /* Expression */

2014-12-26T12:47:02Z

‎Expression

Zhangye: /* Expression */

2014-12-26T12:40:44Z

‎Expression

Zhangye: /* Mutation */

2014-12-26T12:16:53Z

‎Mutation

Zhangye: /* Function */

2014-12-26T12:10:37Z

‎Function

Zhangye at 12:06, 26 December 2014

2014-12-26T12:06:34Z

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 15:19, 26 December 2014
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−	''OsHsfA7'' is a ~~number~~ of heat shock protein (HSP) genes in rice, which play an important role in plant stress tolerance and mainly regulated by heat shock transcription factors (Hsfs) <ref name="ref1"/><ref name="ref2"/>.	+	''OsHsfA7'' is a member of heat shock protein (HSP) genes in rice, which play an important role in plant stress tolerance and mainly regulated by heat shock transcription factors (Hsfs) <ref name="ref1"/><ref name="ref2"/>.

	==Annotated Information==		==Annotated Information==

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 * To examine the tolerance to drought stress, three-week-old plants were withheld water for 10 d and then re-watered for additional 10 d. Both WT and transgenic plants suffered severe blast after un-watering (Fig. 3B).
 * Whereas there was remarkable difference after re-watering. While most of the WT plant leaves showed further withered and could not be rescued, the majority of OsHsfA7-OE plants restored normal growth (Fig. 3C).
-Meanwhile, we tested the thermo-tolerance of transgenic and WT plants at booting stage, no significant difference was found in seed setting rate after treatment<ref name="ref2"/>.
+Meanwhile, we tested the thermo-tolerance of transgenic and WT plants at booting stage, no significant difference was found in seed setting rate after treatment<ref name="ref2"/>.<br>
-# However, the transcript levels of ''OSHsfA4b'', ''OSHsfA5'', ''OSHsfA7'' and five ''OSHsfA2s'' were significantly up-regulated during early stages (''OSHsfA2a'', ''OSHsfA2c'', ''OSHsfA2d'', ''OSHsfA4b'' at 15 min, ''OSHsfA2d'', ''OSHsfA2e'', ''OSHsfA7'' at 45 min and ''OSHsfA5'' at 1.5 h) of heat stress exposure, with the effect gradually diminishing when prolonged heat stress<ref name="ref4"/>.
+However, the transcript levels of ''OSHsfA4b'', ''OSHsfA5'', ''OSHsfA7'' and five ''OSHsfA2s'' were significantly up-regulated during early stages (''OSHsfA2a'', ''OSHsfA2c'', ''OSHsfA2d'', ''OSHsfA4b'' at 15 min, ''OSHsfA2d'', ''OSHsfA2e'', ''OSHsfA7'' at 45 min and ''OSHsfA5'' at 1.5 h) of heat stress exposure, with the effect gradually diminishing when prolonged heat stress<ref name="ref4"/>.<br>
 short-term heat stress and calcium on HSF expression<ref name="ref1"/>:
-* ''OsHsfA7'' has little or no expression when calcium and heat stress are given separately but showed some expression when both these treatments are combined.
+* ''OsHsfA7'' has little or no expression when calcium and heat stress are given separately but showed some expression when both these treatments are combined.<br>
 PEG stress:
-* ''OSHsfA2a'', ''OSHsfA2e'', ''OSHsfA3'', ''OSHsfA4d'', ''OSHsfA5'' together with ''OSHsfA7'' genes responded more drastically by PEG<ref name="ref4"/>
+* ''OSHsfA2a'', ''OSHsfA2e'', ''OSHsfA3'', ''OSHsfA4d'', ''OSHsfA5'' together with ''OSHsfA7'' genes responded more drastically by PEG<ref name="ref4"/><br>
 Among the three ''Hsfs'', ''OsHsfA7'' was significantly upregulated in shoot (11.8-fold) and root(13.3-fold) after SDS. <ref name="ref5"/><br>
 Expression of ''AtHsfA7a'' was reported to be elevated in cells during light stress. ''OsHsfA7'' responded to  all the four treatments(Heat,Salt,PEG,Cold)<ref name="ref6"/>.<br>
 ''OsHsfA2a'', ''OsHsfA2c'', ''OsHsfA2d'', ''OsHsfA7'', ''OsHsfA2f'', ''OsHsfB1'', ''OsHsfB2a'' and OsHsfB4c, genes showed high TA values during HS and OS treatments in Q-PCR<ref name="ref7"/>.<br>
 ''Dheeraj Mittal et al.'' propose that ''OsHsfA2f'' and ''OsHsfA7'' may be involved in the late response to the oxidative stress<ref name="ref7"/>.<br>
 ===Evolution===

@@ Line 43: / Line 43: @@
 ===Knowledge Extension===
+*Plants respond to heat stress by enhancing the expression of genes encoding heat shock protein (HSPs)genes through activation of heat shock factors (HSFs)
 ==Labs working on this gene==

@@ Line 39: / Line 39: @@
 ===Evolution===
-Please input evolution information here.
+*''OsHsfA7'' shares 31.7% and 62.3% identity at the amino acid level with ''HsfA7'' of Medicago and Sorghum. Phylogenetic analysis revealed that genetic relationship between ''Oryza sativa'' and Sorghum was closer<ref name="ref2"/>.
 ===Knowledge Extension===

@@ Line 19: / Line 19: @@
 [[File: OsHsfA7 expression3.png|right|thumb|300px|'''Figure 3.'''Phenotype of the OsHsfA7 over-expression transgenic plants in response to drought treatment.(from reference <ref name="ref2"/>).'']]
-#over-expression of ''OsHsfA7'' can improve salt resistance of transgenic rice<ref name="ref2"/>:
+over-expression of ''OsHsfA7'' can improve salt resistance of transgenic rice<ref name="ref2"/>:
-#*After irrigating with 200 mM NaCl for 10 d, leaf apex of WT plants became brown and dried, whereas over-expressing ''OsHsfA7'' leaves remained green (Fig. 2B).
+* After irrigating with 200 mM NaCl for 10 d, leaf apex of WT plants became brown and dried, whereas over-expressing ''OsHsfA7'' leaves remained green (Fig. 2B).
-#*Leaf REC and MDA content were lower in the transgenic lines than in the WT (Fig. 2E, F).
+* Leaf REC and MDA content were lower in the transgenic lines than in the WT (Fig. 2E, F).
-#*Hydroponic seedlings were treated with 200 mM NaCl for 24 h then transferred to 1/2 MS solution for recovery. After 4 d, leaves of WT were completely curled and wilted, while those of OsHsfA7-OE were rolled only in the tip part (Fig. 2C).
+* Hydroponic seedlings were treated with 200 mM NaCl for 24 h then transferred to 1/2 MS solution for recovery. After 4 d, leaves of WT were completely curled and wilted, while those of OsHsfA7-OE were rolled only in the tip part (Fig. 2C).
-#*After 10 d recovery, leaves of WT were withered and almost all plants were completely dead, while most of the transgenic plants remained alive and only the leaf tips scorched(Fig. 2D).
+* After 10 d recovery, leaves of WT were withered and almost all plants were completely dead, while most of the transgenic plants remained alive and only the leaf tips scorched(Fig. 2D).
-#thermo-tolerance and drought stress<ref name="ref2"/>：
+thermo-tolerance and drought stress<ref name="ref2"/>：
-#*To examine the tolerance to drought stress, three-week-old plants were withheld water for 10 d and then re-watered for additional 10 d. Both WT and transgenic plants suffered severe blast after un-watering (Fig. 3B).
+* To examine the tolerance to drought stress, three-week-old plants were withheld water for 10 d and then re-watered for additional 10 d. Both WT and transgenic plants suffered severe blast after un-watering (Fig. 3B).
-#*Whereas there was remarkable difference after re-watering. While most of the WT plant leaves showed further withered and could not be rescued, the majority of OsHsfA7-OE plants restored normal growth (Fig. 3C).
+* Whereas there was remarkable difference after re-watering. While most of the WT plant leaves showed further withered and could not be rescued, the majority of OsHsfA7-OE plants restored normal growth (Fig. 3C).
-#Meanwhile, we tested the thermo-tolerance of transgenic and WT plants at booting stage, no significant difference was found in seed setting rate after treatment<ref name="ref2"/>.
+Meanwhile, we tested the thermo-tolerance of transgenic and WT plants at booting stage, no significant difference was found in seed setting rate after treatment<ref name="ref2"/>.
-#However, the transcript levels of ''OSHsfA4b'', ''OSHsfA5'', ''OSHsfA7'' and five ''OSHsfA2s'' were significantly up-regulated during early stages (''OSHsfA2a'', ''OSHsfA2c'', ''OSHsfA2d'', ''OSHsfA4b'' at 15 min, ''OSHsfA2d'', ''OSHsfA2e'', ''OSHsfA7'' at 45 min and ''OSHsfA5'' at 1.5 h) of heat stress exposure, with the effect gradually diminishing when prolonged heat stress<ref name="ref4"/>.
+# However, the transcript levels of ''OSHsfA4b'', ''OSHsfA5'', ''OSHsfA7'' and five ''OSHsfA2s'' were significantly up-regulated during early stages (''OSHsfA2a'', ''OSHsfA2c'', ''OSHsfA2d'', ''OSHsfA4b'' at 15 min, ''OSHsfA2d'', ''OSHsfA2e'', ''OSHsfA7'' at 45 min and ''OSHsfA5'' at 1.5 h) of heat stress exposure, with the effect gradually diminishing when prolonged heat stress<ref name="ref4"/>.
+short-term heat stress and calcium on HSF expression<ref name="ref1"/>:
-#short-term heat stress and calcium on HSF expression<ref name="ref1"/>:
+* ''OsHsfA7'' has little or no expression when calcium and heat stress are given separately but showed some expression when both these treatments are combined.
-#*''OsHsfA7'' has little or no expression when calcium and heat stress are given separately but showed some expression when both these treatments are combined.
+PEG stress:
-#PEG stress:
+* ''OSHsfA2a'', ''OSHsfA2e'', ''OSHsfA3'', ''OSHsfA4d'', ''OSHsfA5'' together with ''OSHsfA7'' genes responded more drastically by PEG<ref name="ref4"/>
-#*''OSHsfA2a'', ''OSHsfA2e'', ''OSHsfA3'', ''OSHsfA4d'', ''OSHsfA5'' together with ''OSHsfA7'' genes responded more drastically by PEG<ref name="ref4"/>
+Among the three ''Hsfs'', ''OsHsfA7'' was significantly upregulated in shoot (11.8-fold) and root(13.3-fold) after SDS. <ref name="ref5"/><br>
-#Among the three ''Hsfs'', ''OsHsfA7'' was significantly upregulated in shoot (11.8-fold) and root(13.3-fold) after SDS. <ref name="ref5"/>
+Expression of ''AtHsfA7a'' was reported to be elevated in cells during light stress. ''OsHsfA7'' responded to  all the four treatments(Heat,Salt,PEG,Cold)<ref name="ref6"/>.<br>
-#Expression of ''AtHsfA7a'' was reported to be elevated in cells during light stress. ''OsHsfA7'' responded to  all the four treatments(Heat,Salt,PEG,Cold)<ref name="ref6"/>.
+''OsHsfA2a'', ''OsHsfA2c'', ''OsHsfA2d'', ''OsHsfA7'', ''OsHsfA2f'', ''OsHsfB1'', ''OsHsfB2a'' and OsHsfB4c, genes showed high TA values during HS and OS treatments in Q-PCR<ref name="ref7"/>.<br>
-#''OsHsfA2a'', ''OsHsfA2c'', ''OsHsfA2d'', ''OsHsfA7'', ''OsHsfA2f'', ''OsHsfB1'', ''OsHsfB2a'' and OsHsfB4c, genes showed high TA values during HS and OS treatments in Q-PCR<ref name="ref7"/>.
+''Dheeraj Mittal et al.'' propose that ''OsHsfA2f'' and ''OsHsfA7'' may be involved in the late response to the oxidative stress<ref name="ref7"/>.<br>
 ===Evolution===

@@ Line 16: / Line 16: @@
 ===Expression===
-Please input expression information here.
+[[File: OsHsfA7 expression2.png|right|thumb|300px|'''Figure 2.'''Influence of high salt stress on rice seedlings.(from reference <ref name="ref2"/>).'']]
 ===Evolution===

@@ Line 11: / Line 11: @@
 ===Mutation===
+[[File: OsHsfA7 mutant1.png|left|thumb|300px|'''Figure 1.''' Root morphology of wild-type and OsHsfA7-OE transgenic plants.(from reference <ref name="ref2"/>).'']]
 ===Expression===

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-Please input function information here.
+*''HsfAs'' not only responded to high temperature but also to oxidative stress, high salinity, chilling and other stresses<ref name="ref1"/><ref name="ref2"/><ref name="ref3"/><ref name="ref4"/><ref name="ref5"/><ref name="ref6"/><ref name="ref7"/>.
 ===Mutation===

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+''OsHsfA7'' is a number of heat shock protein (HSP) genes in rice, which play an important role in plant stress tolerance and mainly regulated by heat shock transcription factors (Hsfs) <ref name="ref1"/><ref name="ref2"/>.
 ==Annotated Information==
 ===Function===
 Please input function information here.
 ===Expression===
@@ Line 11: / Line 14: @@
 Please input evolution information here.
-You can also add sub-section(s) at will.
+===Knowledge Extension===
 ==Labs working on this gene==
-Please input related labs here.
+*Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University
 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==