Os01g0667400 - Revision history

192.168.72.52 at 04:51, 14 May 2015

2015-05-14T04:51:43Z

Liying: /* Expression */

2014-05-29T03:43:32Z

‎Expression

Liying: /* Expression */

2014-05-29T03:41:49Z

‎Expression

Liying: /* Expression */

2014-05-29T03:40:59Z

‎Expression

Liying: /* Expression */

2014-05-29T03:40:05Z

‎Expression

Liying: /* Expression */

2014-05-29T03:37:18Z

‎Expression

Liying: /* Expression */

2014-05-29T03:34:38Z

‎Expression

Liying: /* Expression */

2014-05-29T03:31:00Z

‎Expression

Liying: /* Expression */

2014-05-29T03:21:49Z

‎Expression

Liying: /* Function */

2014-05-29T01:55:53Z

‎Function

← Older revision		Revision as of 04:51, 14 May 2015
Line 53:		Line 53:

	==Structured Information==		==Structured Information==
−	~~{{JaponicaGene\|~~	+	[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]
−	~~GeneName = Os01g0667400\|~~
−	~~Description = Conserved hypothetical protein\|~~
−	~~Version = NM_001050343.1 GI:115439056 GeneID:4326320\|~~
−	~~Length = 1538 bp\|~~
−	~~Definition = Oryza sativa Japonica Group Os01g0667400, complete gene.\|~~
−	~~Source = Oryza sativa Japonica Group~~
−
−	~~ORGANISM Oryza sativa Japonica Group~~
−	~~Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;~~
−	~~Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP~~
−	~~clade; Ehrhartoideae; Oryzeae; Oryza.~~
−	\|
−	~~Chromosome = [[:category:Japonica Chromosome 1\|Chromosome 1]]\|~~
−	~~AP = Chromosome 1:29046116..29047653\|~~
−	~~CDS = 29046806..29047498\|~~
−	~~GCID = <gbrowseImage1>~~
−	~~name=NC_008394:29046116..29047653~~
−	~~source=RiceChromosome01~~
−	~~preset=GeneLocation~~
−	~~</gbrowseImage1>\|~~
−	~~GSID = <gbrowseImage2>~~
−	~~name=NC_008394:29046116..29047653~~
−	~~source=RiceChromosome01~~
−	~~preset=GeneLocation~~
−	~~</gbrowseImage2>\|~~
−	CDNA = <cdnaseq>atgtccgtgacgacggccatggacctgctctcgccgctcgccgcggcgtgccaccagcagatgctctatcaaggccagccactggagtcgccgccggcgcctgctcccaaagtgcacggcatcgtgccacacgacgagccggtcttcctgcagtggccgcagagcccctgcctgtcggccgtcgacctcggcgccgccattcttggcggccagtacatgcacctgccggtgcccgctccgcagccaccgtcgtcgccgggcgcggcgggcatgttctgggggctctgcaacgacgtgcaagcgccaaacaacaccggccacaagagctgcgcctggagcgccgggctcggccagcactggtgcggctccgccgatcagctcggcctcggcaagagcagcgcggcgtcgatcgccaccgtgtctaggccggaggaggcgcacgacgtcgacgccacgaagcacggtctgctacagtacggctttggcatcaccacgccgcaagtgcacgtggacgttacctcctcggctgctggcgttctgcctcctgttccgtcctcgccgtcgccgccgaacgccgccgtcaccgtcgcgagcgtggccgccaccgctagcctgactgattttgctgcaagtgctatatctgctggcgccgtcgctaacaatcagtttcaaggtactctttttgttctttga</cdnaseq>\|
−	AA = <aaseq>MSVTTAMDLLSPLAAACHQQMLYQGQPLESPPAPAPKVHGIVPH DEPVFLQWPQSPCLSAVDLGAAILGGQYMHLPVPAPQPPSSPGAAGMFWGLCNDVQAP NNTGHKSCAWSAGLGQHWCGSADQLGLGKSSAASIATVSRPEEAHDVDATKHGLLQYG FGITTPQVHVDVTSSAAGVLPPVPSSPSPPNAAVTVASVAATASLTDFAASAISAGAV ANNQFQGTLFVL</aaseq>\|
−	DNA = <dnaseqindica>156..848#tcacgccgccgccaccaatcctcccggcgccccagccggtgcagccgcagcagcagcttgtctcgcctgtggcggcgcctacctcgtcgtcgtcttcctcctccgaccgttcgtccgggtccagcaagcctgcgagggctacgtcgacgcaggcgatgtccgtgacgacggccatggacctgctctcgccgctcgccgcggcgtgccaccagcagatgctctatcaaggccagccactggagtcgccgccggcgcctgctcccaaagtgcacggcatcgtgccacacgacgagccggtcttcctgcagtggccgcagagcccctgcctgtcggccgtcgacctcggcgccgccattcttggcggccagtacatgcacctgccggtgcccgctccgcagccaccgtcgtcgccgggcgcggcgggcatgttctgggggctctgcaacgacgtgcaagcgccaaacaacaccggccacaagagctgcgcctggagcgccgggctcggccagcactggtgcggctccgccgatcagctcggcctcggcaagagcagcgcggcgtcgatcgccaccgtgtctaggccggaggaggcgcacgacgtcgacgccacgaagcacggtctgctacagtacggctttggcatcaccacgccgcaagtgcacgtggacgttacctcctcggctgctggcgttctgcctcctgttccgtcctcgccgtcgccgccgaacgccgccgtcaccgtcgcgagcgtggccgccaccgctagcctgactgattttgctgcaagtgctatatctgctggcgccgtcgctaacaatcagtttcaaggtactctttttgttctttgatatgaagaatttaattaactggtcaaatcatgaatcaccaccatttagctagattatctgttggcattttttcccctttgttcagtatcacatgcatgcatcatattcatacgctcatgtttaaatggattaattagaagtgtcacttcaggggagtctaacttctcttgtagtgttgtcctcaatttcagtttcgtttggatttaactacaaaatgacaaattttttactgaaagaagaattgtatatgcatgaaattgtactgtactagtttcctttagggcccaatccaaaccagccgttctcaaacaagggccctgtgcccaggcatggcaatgataataagacaagtttaggccatgtaaaatccttttcttttgtgtgtctgtacaatcttatcacttcaatgcagatagactttccagtaaagatgaagaaatttgtcattttgtaagcatgtagatttagacaagaccaaaatttgtgagctgttctccatcaggtcaaattgagtaggtttcttgatgggatgttacaaatatgcgaactactagtactactgctatttcatggtgatggggaacatcatgtcatcgcatgtagatgcattcagctcccacatgctccctccatccaaaaaaaagacaaaccctggtttgcatgtgcaacgtttgaccgtccgtcgtattt</dnaseqindica>\|
−	~~Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050343.1 RefSeq:Os01g0667400]\|~~
−	}}
−	[[Category:Genes]]
−	~~[[Category:Japonica mRNA]]~~
−	[[Category:Oryza Sativa Japonica Group]]
−	[[Category:Japonica ~~Genes]]~~
−	~~[[Category:Japonica Chromosome 1]]~~
−	~~[[Category:~~Chromosome 1]]

@@ Line 20: / Line 20: @@
 Molecular characterization of DWT1:
-[[File:dwt1-5.jpg|100px|frame|center|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
+[[File:dwt1-5.jpg|thumb|100px|frame|center|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
-[[File:dwt1-6.jpg|100px|frame|center|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
+[[File:dwt1-6.jpg|thumb|100px|frame|center|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The morphology of the second internodes of dwt1, wild type, and complemented transgenic plants (DWT1-COM).  E–H. Nuclear localization of DWT1 protein. YFP fluorescence image (E), light view (F), PI stained image (G) and overlay of the three images (H) of Nicotiana benthamiana leaf epidermal cell transformed with the 35S:DWT1-YFP construct.]]
 Expression pattern of DWT1 transcripts and proteins:
-[[File:dwt1-7.jpg|100px|frame|center|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
+[[File:dwt1-7.jpg|thumb|100px|frame|center|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
 type.]]
-[[File:dwt1-8.jpg|100px|frame|center|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
+[[File:dwt1-8.jpg|thumb|100px|frame|center|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
-[[File:dwt1-9.jpg|100px|frame|center|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
+[[File:dwt1-9.jpg|thumb|100px|frame|center|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
 ===Evolution===

@@ Line 20: / Line 20: @@
 Molecular characterization of DWT1:
-[[File:dwt1-5.jpg|300px|frame|center|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
+[[File:dwt1-5.jpg|100px|frame|center|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
-[[File:dwt1-6.jpg|300px|frame|center|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
+[[File:dwt1-6.jpg|100px|frame|center|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
 morphology of the second internodes of dwt1, wild type, and complemented transgenic plants (DWT1-COM).  E–H. Nuclear localization of
 DWT1 protein. YFP fluorescence image (E), light view (F), PI stained image (G) and overlay of the three images (H) of Nicotiana benthamiana leaf epidermal cell transformed with the 35S:DWT1-YFP construct.]]
 Expression pattern of DWT1 transcripts and proteins:
-[[File:dwt1-7.jpg|300px|frame|center|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
+[[File:dwt1-7.jpg|100px|frame|center|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
 type.]]
-[[File:dwt1-8.jpg|300px|frame|center|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
+[[File:dwt1-8.jpg|100px|frame|center|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
-[[File:dwt1-9.jpg|300px|frame|center|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
+[[File:dwt1-9.jpg|100px|frame|center|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
 ===Evolution===

@@ Line 20: / Line 20: @@
 Molecular characterization of DWT1:
-[[File:dwt1-5.jpg|500px|frame|center|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
+[[File:dwt1-5.jpg|300px|frame|center|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
-[[File:dwt1-6.jpg|500px|frame|center|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
+[[File:dwt1-6.jpg|300px|frame|center|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
 morphology of the second internodes of dwt1, wild type, and complemented transgenic plants (DWT1-COM).  E–H. Nuclear localization of
 DWT1 protein. YFP fluorescence image (E), light view (F), PI stained image (G) and overlay of the three images (H) of Nicotiana benthamiana leaf epidermal cell transformed with the 35S:DWT1-YFP construct.]]
 Expression pattern of DWT1 transcripts and proteins:
-[[File:dwt1-7.jpg|500px|frame|center|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
+[[File:dwt1-7.jpg|300px|frame|center|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
 type.]]
-[[File:dwt1-8.jpg|500px|frame|center|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
+[[File:dwt1-8.jpg|300px|frame|center|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
-[[File:dwt1-9.jpg|500px|frame|center|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
+[[File:dwt1-9.jpg|300px|frame|center|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
 ===Evolution===

@@ Line 20: / Line 20: @@
 Molecular characterization of DWT1:
-[[File:dwt1-5.jpg|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
+[[File:dwt1-5.jpg|500px|frame|center|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
-[[File:dwt1-6.jpg|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
+[[File:dwt1-6.jpg|500px|frame|center|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
 morphology of the second internodes of dwt1, wild type, and complemented transgenic plants (DWT1-COM).  E–H. Nuclear localization of
 DWT1 protein. YFP fluorescence image (E), light view (F), PI stained image (G) and overlay of the three images (H) of Nicotiana benthamiana leaf epidermal cell transformed with the 35S:DWT1-YFP construct.]]
 Expression pattern of DWT1 transcripts and proteins:
-[[File:dwt1-7.jpg|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in
+[[File:dwt1-7.jpg|500px|frame|center|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
 type.]]
-[[File:dwt1-8.jpg|300px|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
+[[File:dwt1-8.jpg|500px|frame|center|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
-[[File:dwt1-9.jpg|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
+[[File:dwt1-9.jpg|500px|frame|center|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
 ===Evolution===

@@ Line 19: / Line 19: @@
 The DWT1 gene is highly expressed in young panicles, but undetectable in the internodes, suggesting that DWT1 expression is spatially or temporally separated from its effect on the internode growth. Altered expression of genes involved in cell division and cell elongation, cytokinin/gibberellin homeostasis and signaling have been revealed in DWT1 shorter internodes. Previous reports indicated no effect of these hormonal pathways on the coordination between the tiller and the main shoot growth, but latest resoults reveals that the DWT1 activity is directly or indirectly associated with GA signaling in the internode elongation.
 Molecular characterization of DWT1:
-[[File:dwt1-5.jpg|left|thumb|300px|A. Map-based cloning of the DWT1 gene. The chromosomal region containing DWT1 is diagrammed as the top line with molecular markers shown above the line. The number below the corresponding markers indicates the numbers of recombinants between the markers and DWT1. The BAC clones are shown as overlapping lines. B. Structure of the DWT1 gene. The mutant sequence has one nucleotide C763 deletion in the second exon. Black boxes indicate exons, white boxes indicate UTRs and lines indicate introns. The grey box shows the homeobox domain. ]]
+{| align=center border=1
-[[File:dwt1-6.jpg|left|thumb|300px|C. The plant stature of dwt1, the wild type, and complemented transgenic plants (DWT1-COM). D. The
+|-
 morphology of the second internodes of dwt1, wild type, and complemented transgenic plants (DWT1-COM).  E–H. Nuclear localization of
-DWT1 protein. YFP fluorescence image (E), light view (F), PI stained image (G) and overlay of the three images (H) of Nicotiana benthamiana leaf epidermal cell transformed with the 35S:DWT1-YFP construct.]]
+DWT1 protein. YFP fluorescence image (E), light view (F), PI stained image (G) and overlay of the three images (H) of Nicotiana benthamiana leaf epidermal cell transformed with the 35S:DWT1-YFP construct.
-<br style="clear: left"/>
+</gallery>
 Expression pattern of DWT1 transcripts and proteins:
-[[File:dwt1-7.jpg|left|thumb|300px|A. qRT-PCR analysis of DWT1 gene expression levels in the wild-type tissues including coleoptiles (36 h after seed germination), root tips, mature leaf and sheath, the second internode during elongating (1 cm in length), segments of the upper (U), middle (M), and lower (L) parts of the second internode with 3 cm in length, dormant tiller bud, panicle (less than 1 cm in
+{| align=center border=1
 length), spikelet at stage Sp8 during development of pistil [63], young embryo (10 days after fertilization), and callus with 20–days regeneration. Rice ACTIN1 (OsACTIN1) was used as a control. B. qRT-PCR analysis of DWT1 gene expression levels in young panicle of wild
-type.]]
+type.
-[[File:dwt1-8.jpg|left|thumb|300px|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer. ]]
+File:dwt1-8.jpg|300px|C–L. In situ hybridization of DWT1. Signals were detected in the primary branch meristem (E), secondary branch meristem (C, F), top portion of the panicle (G), shoot apical and radical apical of young embryo (I), and endodermis and exodermis of root tip (K). D, H, J and L were corresponding sections hybridized with the sense probe.I,II,III,IV, the first, second, third and the forth internode, respectively; pb, primary branch meristem; sb, secondary branch meristem; yl, young leave; fm, floral meristem; ifm, inflorescence meristem; exd, exodermis layer; end, endodermis layer.
-[[File:dwt1-9.jpg|left|thumb|300px| M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.]]
+File:dwt1-9.jpg|M–R. YFP fluorescence image of the branch meristem (M–O) and the internode (P–R) in transgenic plants expressing pDWT1:DWT1-YFP. M and P are fluorescence image, N and Q are light view, O and R are overlapping of fluorescence image and light view. S. Western-blot analysis of DWT1 protein levels.
-<br style="clear: left"/>
+</gallery>
 ===Evolution===

← Older revision		Revision as of 01:55, 29 May 2014
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−	~~Please input one-sentence summary here~~.	+	DWARF TILLER1 (DWT1) controls the developmental uniformity of the main shoot and tillers in rice (Oryza sativa).

	==Annotated Information==		==Annotated Information==