Os01g0678500 - Revision history

Xysj2012: /* Structured Information */

2015-05-13T07:24:16Z

‎Structured Information

Lvying: /* References */

2014-06-06T15:07:28Z

‎References

Lvying: /* Cellular localization */

2014-06-06T15:06:36Z

‎Cellular localization

Lvying: /* Mutation */

2014-06-06T15:02:56Z

‎Mutation

Lvying: /* Mutation */

2014-06-06T14:57:13Z

‎Mutation

Jianchao Zhang: /* Mutation */

2014-06-06T14:27:55Z

‎Mutation

Ibpchenwenbo: /* Mutation */

2014-06-06T14:23:20Z

‎Mutation

Ibpchenwenbo: /* Expression */

2014-06-06T14:21:11Z

‎Expression

Huanghs: /* Labs working on this gene */

2014-06-03T09:37:50Z

‎Labs working on this gene

Huanghs: /* Labs working on this gene */

2014-06-03T09:36:53Z

‎Labs working on this gene

@@ Line 67: / Line 67: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 62: / Line 62: @@
 <ref name="ref4">
 Hamada H, Kurusu T, Okuma E, Nokajima H, Kiyoduka M, Koyano T, Sugiyama Y, Okada K, Koga J, Saji H, Miyao A, Hirochika H, Yamane H, Murata Y, Kuchitsu K. Regulation of a proteinaceous elicitor-induced Ca2+ influx and production of phytoalexins by a putative voltage-gated cation channel, OsTPC1, in cultured rice cells. J Biol Chem. 2012 Mar 23;287(13):9931-9. </ref>
 </references>

@@ Line 29: / Line 29: @@
 ===Cellular localization===
 To study the intracellular localization of the OsTPC1 protein, ''Takamitsu Kurusu et al'' introduced the green fluorescent protein (GFP) construct fused to the N-terminus of OsTPC1 into onion epidermal cells and examined its intracellular localization by confocal laser scanning microscopy. When GFP alone was expressed, it localized to the nucleus and the cytoplasm[Fig-7 a-c],In contrast, the GFP-OsTPC1fusion protein was specifically targeted to the plasma membrane in patches (Figure-7 d–f), suggesting that OsTPC1 functions at the plasma membrane.<ref name="ref2" />
 ==Labs working on this gene==
 *Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo, 184-8588 Japan

← Older revision		Revision as of 15:02, 6 June 2014
Line 8:		Line 8:

	===Mutation===		===Mutation===
		+	[[File:Fig-6 zhoujujun.jpg\|right\|thumb\|250px\|Fig-6]]
	1.The OsTPC1overexpressors showed remarkably slowed growth and reduced fertility in adult plants[Fig-1 C,D].These phenotypes were exhibited in all six independent T1 transgenic lines and the expression level of the OsTPC1transcripts in the 35S::OsTPC1 plants well correlated with the severity of the phenotypes[Fig-1B,C]. OsTPC1 overexpressing lines showing severe phenotype also displayed a dwarf phenotype with dark green leaves in adult plants, and green colored roots in the seedling stage under light condition[Fig-1E] <ref name="ref1" />		1.The OsTPC1overexpressors showed remarkably slowed growth and reduced fertility in adult plants[Fig-1 C,D].These phenotypes were exhibited in all six independent T1 transgenic lines and the expression level of the OsTPC1transcripts in the 35S::OsTPC1 plants well correlated with the severity of the phenotypes[Fig-1B,C]. OsTPC1 overexpressing lines showing severe phenotype also displayed a dwarf phenotype with dark green leaves in adult plants, and green colored roots in the seedling stage under light condition[Fig-1E] <ref name="ref1" />

← Older revision		Revision as of 14:57, 6 June 2014
Line 20:		Line 20:

	Fig-2:Isolation of Tos17insertional mutant of the OsTPC1. (A) The structure of the OsTPC1gene and insertion site of Tos17in the OsTPC1 gene of the mutant line (NF1041). The exon and intron regions of OsTPC1are presented as closed and open bars, respectively. (B) A schematic representation of OsTPC1mRNA and the location of the deletion in the Ostpc1mutant. The approximate locations of primer sequences are shown by arrows. (C) PCR analysis of OsTPC1mRNA in Ostpc1and wild type. PCR products generated by using primers exon11P and P2, which amplify the mRNA region derived fromthe splicing of exon 11 to exon 21. (D) Yeast complementation analysis using Ostpc1cDNA. Square, K927 (cch1)/pYES2; circle, K927 (cch1)/pYES2-OsTPC1; triangle, K927 (cch1)/pYES2-Ostpc1. Vertical bars represent SE (n= 3) <ref name="ref1" />		Fig-2:Isolation of Tos17insertional mutant of the OsTPC1. (A) The structure of the OsTPC1gene and insertion site of Tos17in the OsTPC1 gene of the mutant line (NF1041). The exon and intron regions of OsTPC1are presented as closed and open bars, respectively. (B) A schematic representation of OsTPC1mRNA and the location of the deletion in the Ostpc1mutant. The approximate locations of primer sequences are shown by arrows. (C) PCR analysis of OsTPC1mRNA in Ostpc1and wild type. PCR products generated by using primers exon11P and P2, which amplify the mRNA region derived fromthe splicing of exon 11 to exon 21. (D) Yeast complementation analysis using Ostpc1cDNA. Square, K927 (cch1)/pYES2; circle, K927 (cch1)/pYES2-OsTPC1; triangle, K927 (cch1)/pYES2-Ostpc1. Vertical bars represent SE (n= 3) <ref name="ref1" />
		+
		+	3.By performing elicitor sensitivity in both transgenic cultured cells overexpressing OsTPC1, it indicates that the elicitor-induced browning of the cells was much more evident in OsTPC1-overexpressing cells than in b-glucuronidase (GUS)-expressing control cells, and theirdarkness corresponded to theOsTPC1expression level in35S:OsTPC1 transgenic cells[Fig-5 a,b]. However in the Tos17 insertional mutant of the OsTPC1 cells,cell browning was remarkably reduced and the mitochondrial reductase activity is not induced, even at concentrations of TvX up to 120ug/ml[Fig6 a,c]. <ref name="ref2" />

	===Expression===		===Expression===

@@ Line 14: / Line 14: @@
 Fig-1:Phenotypes of plants overexpressing OsTPC1cDNA. (A) The OsTPC1expression vector for rice transformation. 35S P, CaMV 35S promoter; OsTPC1, ORF regions (2,274 bp in size) for OsTPC1cDNA; nos T, terminator sequence of the gene for nopaline synthase. 35S::GUSwas
 used as vector controls. (B) RT-PCR analysis of OsTPC1in the plants with sense OsTPC1. ActincDNA was used as a control. (C) Phenotype of overexpressing OsTPC1lines (T1) at adult stage. Plants were grown for 60 d in the greenhouse. Right: plant overexpressing OsTPC1mRNA and left: control plant overexpressing GUSgene. White bars indicate 10 cm. (D) Phenotype of overexpressing OsTPC1lines (T1) at seedling stage. Seedlings were grown for 10 d in the MS medium (16 h light/8 h darkness, 28℃). White bars indicate 10 cm. (E) Root greening observed in overexpressing OsTPC1lines (T1). Seedlings were grown for 14 d in the MS medium (continuous light, 28℃). Red bars indicate 1 mm. Control lines:C1-1 and C2-5, OsTPC1overexpressors: S2-3, S2-4 and S2-5.<ref name="ref1" />
 ===Expression===

@@ Line 9: / Line 9: @@
 ===Mutation===
 .The OsTPC1overexpressors showed remarkably slowed growth and reduced fertility in adult plants[Fig-1 C,D].These phenotypes were exhibited in all six independent T1 transgenic lines and the expression level of the OsTPC1transcripts in the 35S::OsTPC1 plants well correlated with the severity of the phenotypes[Fig-1B,C]. OsTPC1 overexpressing lines showing severe phenotype also displayed a dwarf phenotype with dark green leaves in adult plants, and green colored roots in the seedling stage under light condition[Fig-1E] <ref name="ref1" />
 ===Expression===
 Expression of the OsTPC1mRNA is detected at a similar level ubiquitously in calli, mature leaves and shoots except for roots where its expression was weaker.[Fig-4] <ref name="ref1" />

@@ Line 21: / Line 21: @@
 *Genome & Drug Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
 *Graduate School of Natural Science and Technology, Okayama University, Okayama,700-8530, Japan
-*�Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
+*Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
 *Food Science Research Laboratories, Meiji Co., Ltd., 5-3-1, Chiyoda, Sakado, Saitama 350-0289, Japan
 *Environmental Biology Division, National Institute for Environmental Studies, Tsukuba, Ibaraki, 305-8506 Japan

@@ Line 17: / Line 17: @@
 *Department of Biology, Tokyo Gakugei University, Tokyo, 184-8501 Japan
 *CREST, Japan Science and Technology Agency, Saitama, 332-0012 Japan
 ==References==