Os01g0726400 - Revision history

Xysj2017 at 14:05, 14 February 2017

2017-02-14T14:05:42Z

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Xysj2012: /* Structured Information */

2015-05-13T07:27:04Z

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Amy: /* Annotated Information */

2014-06-07T05:32:38Z

‎Annotated Information

Amy: /* Labs working on this gene */

2014-06-07T05:13:18Z

‎Labs working on this gene

Amy: /* References */

2014-06-07T05:08:40Z

‎References

Amy: /* Annotated Information */

2014-06-07T05:06:28Z

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2014-06-07T05:00:27Z

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2014-06-07T04:58:58Z

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2014-06-07T04:57:43Z

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Amy: /* Evolution */

2014-06-07T04:55:39Z

‎Evolution

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 05:32, 7 June 2014
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	==Annotated Information==		==Annotated Information==
		+	CHIMERIC FLORAL ORGANS (CFO1), one kind of MIKC-type MADS box gene,which plays a key role in the regulation of floral organ identity in rice (Oryza sativa). Map-based cloning demonstrated that CFO1 encoded the OsMADS32 protein. Phylogenetic analysis revealed that CFO1/OsMADS32 belonged to a monocot-specific clade in the MIKC-type MADS box gene family. The expression domains of CF. We propose that the CFO1 gene plays a pivotal role in maintaining floral organ identity through negative regulation of DL expression.
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	Functions:		Functions:

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 )Agrawal GK, Abe K, Yamazaki M, Miyao A, Hirochika H. (2005) Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene. Plant Mol Biol 59: 125–135
 )Bommert P, Satoh-Nagasawa N, Jackson D, Hirano HY. (2005) Genetics and evolution of inflorescence and flower development in grasses. Plant Cell Physiol 46: 69–78
 ==Structured Information==

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 Please input related labs here.
-==References==
+)Agrawal GK, Abe K, Yamazaki M, Miyao A, Hirochika H. (2005) Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene. Plant Mol Biol 59: 125–135
-Please input cited references here.
+)Bommert P, Satoh-Nagasawa N, Jackson D, Hirano HY. (2005) Genetics and evolution of inflorescence and flower development in grasses. Plant Cell Physiol 46: 69–78
 ==Structured Information==

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 ==Annotated Information==
 ) CFO1 Is an Important Regulator of Floral Organ Identity in Rice
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 In cfo1 flowers, the initiation and development of floral organ primordia in whorls 3 and 4 were delayed, and the numbers of stamens and pistils were altered, which indicated that normal floral meristem development was disturbed. However, compared with the above-mentioned mutants, floral meristem determinacy was affected less by mutation of CFO1, because no repeated organs developed and the floral meristem was consumed by the pistils in cfo1 flowers. These results suggest that CFO1 is required for normal floral meristem development but contributes very weakly to the regulation of floral meristem determinacy.
 We investigated CFO1 expression in wild-type plants using in situ hybridization. The CFO1 gene was expressed initially in the primary and secondary branch meristems (Fig. 9D).Abundant CFO1 transcripts were detected subsequently in the meristems of spikelets and florets (Fig. 9, E and F). During stages Sp6 and Sp7, when the stamen primordia were initiated, analysis of serial longitudinal and transverse sections indicated that the expression signals of CFO1 were focused on several specific domains and organs (Fig. 9, G–K). First, CFO1 was expressed strongly in organ primordia, such as rudimentary glumes (Fig. 9, G and H), the mrp (lines in Fig. 9, I–K), lodicules (triangles in Fig. 9, G, H, and L), upper and central regions of stamens (asterisks in Fig. 9, I–K), and the upper region of the pistil (Fig. 9, G–K). Second, CFO1 was expressed in vascular bundles of floral organ primordia, such as the rachilla (arrows in Fig. 9G), sterile lemma (arrows in Fig. 6I),
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 [[File:Fig9.png]]
 Genetic analysis demonstrated that the cfo1trait was controlled by a single recessive gene.The cfo1 locus was mapped on the short arm of chromosome 1 within an approximately 645-kb region between the simple sequence repeat markers RM1152 and RM128 (Fig. 7, A and B). Fortunately, a gene that encodes a 196-residue MADS box transcription factor, OsMADS32, occurs in this region. Sequence comparison revealed that OsMADS32 in the cfo1 mutant was missing a single nucleotide (T) at position 214 of the open reading frame, which caused a premature translation stop (Fig. 7, C and D). An RsaI restriction site in the wild-type allele was abolished in the cfo1 mutant allele because of this single-nucleotide deletion (Fig. 7C).
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 CFO1 Belongs to a Monocot-Specific Class.The MIKC-type genes are classified as a plant-specific subfamily of the MADS box gene family. This subfamily contains a well-conserved MADS (M) domain and three additional plant-specific domains: an intervening (I) domain, a keratin-like coiled-coil (K) domain, and a C-terminal (C) domain. The MIKC-type genes can be further divided into two subgroups, MIKCC and MIKC*, based on the intron-exon structure(Fig8). The 153 MIKCC-type genes were divided into 13 distinct classes: A or AP1-like class (A-AP1), B-AP3, B-PI, Bs-TT16, C/D-AG, E-SEP, F-SOC1, T-SVP, AGL6-like, AGL12-like, ANR1-like, FLC-like, and CFO1-like (Fig. 8). Interestingly, CFO1 and its orthologs from grasses and P. dactylifera constituted the earliest diverging CFO1-like class in the MIKCC family (Fig. 8). This class included Pd from P. dactylifera, TaAGL14andTaAGL15from wheat, ZmMADS32 and ZmLOC100279931 from maize, HvWM16 from barley, Sb03g03380 from S. bicolor, Bd12g48690 fromBrachypodium distachyon, and CFO1 from rice (Fig. 8). These results suggest that the CFO1-like gene class is monocot specific.
 ==Labs working on this gene==

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 [[File:Fig7.png]]
-CFO1 Belongs to a Monocot-Specific Class.The MIKC-type genes are classified as a plant-specific subfamily of the MADS box gene family. This subfamily contains a well-conserved MADS (M) domain and three additional plant-specific domains: an intervening (I) domain, a keratin-like coiled-coil (K) domain, and a C-terminal (C) domain. The MIKC-type genes can be further divided into two subgroups, MIKCC and MIKC*, based on the intron-exon structure. (Fig8)
+CFO1 Belongs to a Monocot-Specific Class.The MIKC-type genes are classified as a plant-specific subfamily of the MADS box gene family. This subfamily contains a well-conserved MADS (M) domain and three additional plant-specific domains: an intervening (I) domain, a keratin-like coiled-coil (K) domain, and a C-terminal (C) domain. The MIKC-type genes can be further divided into two subgroups, MIKCC and MIKC*, based on the intron-exon structure(Fig8). The 153 MIKCC-type genes were divided into 13 distinct classes: A or AP1-like class (A-AP1), B-AP3, B-PI, Bs-TT16, C/D-AG, E-SEP, F-SOC1, T-SVP, AGL6-like, AGL12-like, ANR1-like, FLC-like, and CFO1-like (Fig. 8). Interestingly, CFO1 and its orthologs from grasses and P. dactylifera constituted the earliest diverging CFO1-like class in the MIKCC family (Fig. 8). This class included Pd from P. dactylifera, TaAGL14andTaAGL15from wheat, ZmMADS32 and ZmLOC100279931 from maize, HvWM16 from barley, Sb03g03380 from S. bicolor, Bd12g48690 fromBrachypodium distachyon, and CFO1 from rice (Fig. 8). These results suggest that the CFO1-like gene class is monocot specific.
 ==Labs working on this gene==

← Older revision		Revision as of 04:57, 7 June 2014
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−	Genetic analysis demonstrated that the cfo1trait was controlled by a single recessive gene.The cfo1 locus was mapped on the short arm of chromosome 1 within an approximately 645-kb region between the simple sequence repeat markers RM1152 and RM128 (Fig. 7, A and B). Fortunately, a gene that encodes a 196-residue MADS box transcription factor, OsMADS32, occurs in this region. Sequence comparison revealed that OsMADS32 in the cfo1 mutant was missing a single nucleotide (T) at position 214 of the open reading frame, which caused a premature translation stop (Fig. 7, C and D). An RsaI restriction site in the wild-type allele was abolished in the cfo1 mutant allele because of this single-nucleotide deletion (Fig. 7C). CFO1 Belongs to a Monocot-Specific Class.The MIKC-type genes are classified as a plant-specific subfamily of the MADS box gene family. This subfamily contains a well-conserved MADS (M) domain and three additional plant-specific domains: an intervening (I) domain, a keratin-like coiled-coil (K) domain, and a C-terminal (C) domain. The MIKC-type genes can be further divided into two subgroups, MIKCC and MIKC*, based on the intron-exon structure. (Fig8)	+	Genetic analysis demonstrated that the cfo1trait was controlled by a single recessive gene.The cfo1 locus was mapped on the short arm of chromosome 1 within an approximately 645-kb region between the simple sequence repeat markers RM1152 and RM128 (Fig. 7, A and B). Fortunately, a gene that encodes a 196-residue MADS box transcription factor, OsMADS32, occurs in this region. Sequence comparison revealed that OsMADS32 in the cfo1 mutant was missing a single nucleotide (T) at position 214 of the open reading frame, which caused a premature translation stop (Fig. 7, C and D). An RsaI restriction site in the wild-type allele was abolished in the cfo1 mutant allele because of this single-nucleotide deletion (Fig. 7C).
		+	[[File:Fig7.png]]CFO1 Belongs to a Monocot-Specific Class.The MIKC-type genes are classified as a plant-specific subfamily of the MADS box gene family. This subfamily contains a well-conserved MADS (M) domain and three additional plant-specific domains: an intervening (I) domain, a keratin-like coiled-coil (K) domain, and a C-terminal (C) domain. The MIKC-type genes can be further divided into two subgroups, MIKCC and MIKC*, based on the intron-exon structure. (Fig8)

	==Labs working on this gene==		==Labs working on this gene==

← Older revision		Revision as of 04:55, 7 June 2014
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−	~~===Evolution===~~	+	Genetic analysis demonstrated that the cfo1trait was controlled by a single recessive gene.The cfo1 locus was mapped on the short arm of chromosome 1 within an approximately 645-kb region between the simple sequence repeat markers RM1152 and RM128 (Fig. 7, A and B). Fortunately, a gene that encodes a 196-residue MADS box transcription factor, OsMADS32, occurs in this region. Sequence comparison revealed that OsMADS32 in the cfo1 mutant was missing a single nucleotide (T) at position 214 of the open reading frame, which caused a premature translation stop (Fig. 7, C and D). An RsaI restriction site in the wild-type allele was abolished in the cfo1 mutant allele because of this single-nucleotide deletion (Fig. 7C). CFO1 Belongs to a Monocot-Specific Class.The MIKC-type genes are classified as a plant-specific subfamily of the MADS box gene family. This subfamily contains a well-conserved MADS (M) domain and three additional plant-specific domains: an intervening (I) domain, a keratin-like coiled-coil (K) domain, and a C-terminal (C) domain. The MIKC-type genes can be further divided into two subgroups, MIKCC and MIKC*, based on the intron-exon structure. (Fig8)
−	~~Please input evolution information here~~.
−
−	~~You can also add sub~~-~~section~~(s) at ~~will~~.

	==Labs working on this gene==		==Labs working on this gene==