Os01g0757200 - Revision history

Xysj1980: /* Annotated Information */

2017-08-24T05:56:46Z

‎Annotated Information

Xysj1980 at 05:56, 24 August 2017

2017-08-24T05:56:31Z

Xysj1980: /* Annotated Information */

2017-08-24T05:56:02Z

‎Annotated Information

Xysj1980: /* Function */

2017-08-24T05:55:30Z

‎Function

Guangyi: /* Expression */

2016-07-26T06:49:36Z

‎Expression

Guangyi: /* Expression */

2016-07-26T06:49:00Z

‎Expression

Guangyi: /* Function */

2016-07-26T06:47:46Z

‎Function

Guangyi: /* Function */

2016-07-26T06:45:36Z

‎Function

Guangyi: /* Function */

2016-07-26T06:44:16Z

‎Function

Guangyi: /* Expression */

2016-07-26T06:39:32Z

‎Expression

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 ==Annotated Information==
 ===Function===
 The members of the rice (Oryza sativa) GA2ox family are differentially regulated and act in concert or individually to control GA levels during flowering, tillering, and seed germination.
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 The severe dwarf mutant M77777, designated as GA2ox3ACT,carries a T-DNA insertion at a position 587 bp upstream of the translation start codon of GA2ox3 (Figure 5A). Accumulation of GA2ox3 mRNA was significantly enhanced in the heterozygous mutant. The GA2ox3ACT mutant did not produce seeds and was therefore maintained and propagated vegetatively.
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 ===Expression===

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 Genes GA2ox3 was differentially expressed in leaves, and its expression was also temporally regulated (Figure 3B).
 GA2ox3 accumulation of its mRNAs in leaves was detected prior to the transition from vegetative to reproductive growth phases.Since expression of most GA2oxs terminated after the active tillering stage, the pattern of tiller growth throughout the rice life cycle was examined. Tiller number increased from 30 to 50 DAI (active tillering), remained constant until 75 DAI, and then increased again until 90 DAI (late tillering)when the experiment was terminated (Figure 3C). Expression of each group of GA2oxs paralleled the active and late tillering stages (cf. Figures 3B with 3C). Germination was observed from 1 DAI and reached almost 100% at 2 DAI (Figure 4A). The accumulation of most GA2ox mRNAs was detectable starting from 0 to 1 DAI and maintained at similar levels afterward, except that of GA2ox5 and GA2ox9 was moderately reduced at 2 DAI (Figure 4B).
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 [[File:1-f4b.png |center |thumb |10000px |''''''Figure 4. C20 GA2oxs Could Be Responsible for Regulating Seed Germination.(B) Expression patterns of GA2oxs in rice seedlings between 0 and ;5 DAI. Total RNA was isolated from embryos at each time point and
 analyzed by RT-PCR. The 18S rRNA gene (rRNA) was used as a control.'''''']]
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 ===Evolution===
 ==Labs working on this gene==

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 The severe dwarf mutant M77777, designated as GA2ox3ACT,carries a T-DNA insertion at a position 587 bp upstream of the translation start codon of GA2ox3 (Figure 5A). Accumulation of GA2ox3 mRNA was significantly enhanced in the heterozygous mutant. The GA2ox3ACT mutant did not produce seeds and was therefore maintained and propagated vegetatively.
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-[[File:1-f5aa.png |center |thumb |10000px |''''''Figure 5. Severely Dwarfed and Semidwarfed Rice Mutants Obtained by T-DNA Activation Tagging.(A) The severe dwarf mutant GA2ox3ACT (M77777).'''''']]
+[[File:1-f5aa.png |right |thumb |300px |''''''Figure 5. Severely Dwarfed and Semidwarfed Rice Mutants Obtained by T-DNA Activation Tagging.(A) The severe dwarf mutant GA2ox3ACT (M77777).'''''']]
 ===Expression===

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 [[File:1-f3c.png |left |thumb |10000px |''''''Figure 3. Differential Expression of Two Groups of GA2oxs Regulates Flower and Tiller Development.(C) Tiller development during the life cycle of rice. A total of eight plants were used for counting tiller number, and error bars indicate the SE of the mean at each time point.'''''']]
 [[File:1-f4a.png |center |thumb |10000px |''''''Figure 4. C20 GA2oxs Could Be Responsible for Regulating Seed Germination.(A) Germination rate of rice seeds reached 100% at 2 DAI.'''''']]
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 The members of the rice (Oryza sativa) GA2ox family are differentially regulated and act in concert or individually to control GA levels during flowering, tillering, and seed germination.
 <br>
-The three activation-tagged mutants, GA2ox5D335-341ACT,GA2ox6ACT, and GA2ox9ACT, were further characterized. Progenies displayed the same phenotypes as their parents, with GA2ox5D335-341ACT and GA2ox9ACT growing slightly shorter than the wild type, while GA2ox6ACT remained severely dwarfed throughout all growth stages (Figures 6A and 6B). GA2ox5D335-341ACT and GA2ox9ACT displayed a normal height but had longer roots and higher tiller numbers than the wild type (Table 1). Other traits significantly altered in the severe dwarf GA2ox6ACT mutant included shorter leaves, later heading date, reduced panicle length, higher tiller numbers, lower grain weight, and lower seed fertility compared with the wild type (Table 1). Germination of GA2ox6ACT seeds was also significantly delayed, as it took 20 d to reach 90% germination rate, while the wild-type and GA2ox9ACT mutant seeds took only 2 d to reach a germination rate of 97 and 98%, respectively (Figure 6C). Germination of GA2ox5D335-341ACT seeds was delayed for 4 d to reach a final 88% germination rate (Figure 6C).
+The severe dwarf mutant M77777, designated as GA2ox3ACT,carries a T-DNA insertion at a position 587 bp upstream of the translation start codon of GA2ox3 (Figure 5A). Accumulation of GA2ox3 mRNA was significantly enhanced in the heterozygous mutant. The GA2ox3ACT mutant did not produce seeds and was therefore maintained and propagated vegetatively.
 <br>
 [[File:1-t1.png |center |thumb |10000px ]]