Os01g0797600 - Revision history

Xysj2019: /* Function */

2017-03-06T09:18:38Z

‎Function

Xysj2019 at 09:17, 6 March 2017

2017-03-06T09:17:51Z

Xysj2019 at 09:14, 6 March 2017

2017-03-06T09:14:11Z

Xysj2019 at 09:12, 6 March 2017

2017-03-06T09:12:55Z

Zhangs: /* References */

2014-05-25T07:17:25Z

‎References

Zhangs: /* Labs working on this gene */

2014-05-25T07:15:55Z

‎Labs working on this gene

Zhangs: /* Expression */

2014-05-25T07:15:01Z

‎Expression

Zhangs: /* References */

2014-05-25T07:12:02Z

‎References

Zhangs: /* References */

2014-05-25T07:08:32Z

‎References

Zhangs: /* Labs working on this gene */

2014-05-25T07:06:43Z

‎Labs working on this gene

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 ===Function===
-*OsERF3 (Os01g0797600), is AP2 domain-containing protein, it is found to be significantly up-regulated in both genotypes under cold stress.<ref name="ref1" />
+*'''''AP37''''' (Os01g0797600), is AP2 domain-containing protein, it is found to be significantly up-regulated in both genotypes under cold stress.<ref name="ref1" />
-*OsERF3 (Os01g0797600) belongs to class II ERFs with a conserved EAR-motif (DLNRPPP) at the C terminal (Nakano et al. 2006). In rice, OsERF3 participated in the resistance to disease and the herbivore-induced defense responses via mediating different hormone pathways (Luet al. 2011).
+*'''''AP37''''' gene has the potential to improve drought tolerance in rice without causing undesirable growth phenotypes.
 ===Expression===

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 ===Expression===
-*The expression of OsERF3 was induced by drought, salt, ACC and ABA treatment. In addition, it showed a higher expression level in the root and sheath than that in the leaf.<ref name="ref2" />
+* The overexpression of '''''AP37''''' and '''''AP59''''' in rice under the control of the constitutive promoter OsCc1 increased the tolerance to drought and high salinity at the vegetative stage.
-*The full length of OsERF3 gene was obtained from the cDNA library using specific primers.The full length of OsERF3 gene was obtained from the cDNA library using specific primers. The mutation of EAR motif of OsERF3 (mEAR) was obtained by replacing the base of A680 with C (Fig. 1a) through PCR amplifications using specific primers (Table S1). Then, the full-length cDNA of OsERF3 and mEAR were inserted into pCAMBIA1307 modified from pCAMBIA1300, respectively. For the modification of pCAMBIA1307 vector (Fig. S1), we inserted a fragment including a CaMV 35S promoter and multiple cloning sites from vector pBluescript into the SstI and KpnI of pCAMBIA1300 vector. To generate the ERF3:GUS construct, the 1.0 kb promoter region upstream to start codon of OsERF3 was amplified using specific primers (OsERF3P-F and OsERF3P-R) (Table S1). Then, the fragment digested with EcoRI and NcoI was cloned into the multiple cloning sites of pCAMBIA1391Z vector. All of the transgenic rice plants were generated through Agrobacterium-mediated transformation. T1 seedlings were examined by PCR amplifications at the DNA and RNA levels with specific primers (Table S1); T2 and T3 seeds were screened by germinating with the selection of 50 ng/l hygromycin.<ref name="ref2" />
+* Increased tolerance to low temperatures was observed only in OsCc1:AP37 plants. More importantly, the OsCc1:AP37 plants showed significantly enhanced drought tolerance in the field, which increased grain yield by 16% to 57% over controls under severe drought conditions, yet exhibited no significant difference under normal growth conditions.
-[[File:zch.png|200px|thumb|left|Fig. 1. Comparison of EAR motif and phylogenetic analysis of EAR motif containing ERF proteins from rice and Arabidopsis.]]
+* In contrast, grain yield in OsCc1:AP59 plants in the field was reduced by 23% to 43% compared with controls under both normal and drought stress conditions.
-*The expression of OsERF3 was highly induced by ABA at 1 h, and reached peak at 2 h. Similarly, it was rapidly inducible by ACC at 0.5 h and peaked at 2 h, and then it decreased to normal level at 8 h (Fig. 2a). Moreover, OsERF3 was up-regulated by drought and peaked at 2 h. Similarly, OsERF3 was quickly induced by high salinity and peaked at 0.5 h. These results indicate that OsERF3 might be associated with ethylene- and ABArelated environmental stimuli in rice.the potential cis-elements withinthe 1 kb region (1,040 bp) upstream to the start codon(Table S2). There were more important elements involved in different hormones or stress signaling (including ethylene, ABA, Auxin and GA as well as dehydration, cold and light) and specific tissues. These data also support the molecular cues for the expression of OsERF3 in response to different hormones and stress treatments.<ref name="ref2" />
+* Microarray experiments identified 10 and 38 genes that are up-regulated by AP37 and AP59, respectively, in addition to 37 genes that are commonly induced by both factors.
 ==Labs working on this gene==
-*Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing100081, China
+* School of Biotechnology and Environmental Engineering, Myongji University, Yongin 449–728, Korea
 ==References==

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 <references>
 * <ref name="ref1">
-Zhang et al. BMC Genomics 2012, 13:461.Fan Zhang, Liyu Huang, Wensheng Wang, Xiuqin Zhao, Linghua Zhu, Binying Fu and Zhikang Li;Genome-wide gene expression profiling of introgressed indica rice alleles associated with seedling cold tolerance improvement in a japonica rice background
+Oh SJ, Kim YS, Kwon CW, Park HK, Jeong JS, Kim JK. Overexpression of the
-</ref>
+transcription factor AP37 in rice improves grain yield under drought conditions.
-* <ref name="ref2">
+Plant Physiol. 2009 Jul;150(3):1368-79. doi: 10.1104/pp.109.137554. PubMed PMID:
-Haiwen Zhang • Jianfei Zhang • Ruidang Quan •Xiaowu Pan • Liyun Wan • Rongfeng Huang;EAR motif mutation of rice OsERF3 alters the regulation of ethylene biosynthesis and drought tolerance. Planta (2013) 237:1443–1451
+19429605; PubMed Central PMCID: PMC2705040.
 </ref>
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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-Please input one-sentence summary here.
+The rice '''''Os01g0797600''''' was reported as '''''AP37''''' in 2009 <ref name="ref1" /> by researchers from Korea.
 ==Annotated Information==
 ===Function===
 *OsERF3 (Os01g0797600), is AP2 domain-containing protein, it is found to be significantly up-regulated in both genotypes under cold stress.<ref name="ref1" />
-*OsERF3 (Os01g0797600) belongs to class II ERFs with a conserved EAR-motif (DLNRPPP) at the C terminal (Nakano et al. 2006). In rice, OsERF3 participated in the resistance to disease and the herbivore-induced defense responses via mediating different hormone pathways (Luet al. 2011).We also found that OsERF3 negatively
+*OsERF3 (Os01g0797600) belongs to class II ERFs with a conserved EAR-motif (DLNRPPP) at the C terminal (Nakano et al. 2006). In rice, OsERF3 participated in the resistance to disease and the herbivore-induced defense responses via mediating different hormone pathways (Luet al. 2011).
 *OsERF3 is a transcriptional repressor with an ethylene-responsive element-binding factor-associated amphiphilic repression (EAR) motif (F/LDLNxxP), which transcriptionally represses the ethylene emission and drought tolerance in rice. However, its molecular mechanism to explore repression function remains unknown.<ref name="ref2" />
-*OsERF3, an AP2 (APETALA 2 Gene)/ERF transcription factor, binds the LRK6 promoter at this new motif, which might cause differential expression of LRK6 in the 93-11/Nipponbare hybrid.The DNA binding ability ofOsERF3’s ERF/AP2 domain was analysed using the one-hybrid assay.<ref name="ref3" />
+*OsERF3, is an AP2 (APETALA 2 Gene)/ERF transcription factor, binds the LRK6 promoter at this new motif, which might cause differential expression of LRK6 in the 93-11/Nipponbare hybrid.The DNA binding ability ofOsERF3’s ERF/AP2 domain was analysed using the one-hybrid assay.<ref name="ref3" />
 ===Expression===

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 * <ref name="ref3">
 QIANJIE WANG, WEIWEI QI, YING WANG, FAN SUN, XIAOYIN QIAN,XIAOJIN LUO AND JINSHUI YANG;Isolation and identification of an AP2/ERF factor that binds an allelic cis-element of rice gene LRK6;Genet. Res., Camb. (2011), 93, pp. 319–332.
 </ref>
 ==Structured Information==

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 *Rice Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China
 *State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai 200433, China.
 ==References==

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 [[File:zch2.png|400px|thumb|right|Fig. 2.Expression of OsERF3 .]]
 *The OsERF3 gene was predicted to encode a protein of 235 amino acids, with a calculated molecular weight of 24.3 kDa. OsERF3 is an AP2/ERF protein with an N-terminal domain containing one highly conserved ERF motif, and a C-terminus containing an y30 amino acid domain comprising a conserved ERF-associated amphiphilic repression(EAR) motif .<ref name="ref3" />
 ===Mutation===