Os01g0831900 - Revision history

192.168.72.52 at 05:01, 14 May 2015

2015-05-14T05:01:11Z

Little baby: /* References */

2014-06-05T02:58:46Z

‎References

Little baby: /* Labs working on this gene */

2014-06-05T02:58:17Z

‎Labs working on this gene

Little baby: /* Evolution */

2014-06-05T02:57:55Z

‎Evolution

Little baby: /* Expression */

2014-06-05T02:57:34Z

‎Expression

Little baby: /* Function */

2014-06-05T02:56:44Z

‎Function

Little baby: /* Expression */

2014-06-05T02:54:32Z

‎Expression

Little baby: /* Function */

2014-06-05T02:53:41Z

‎Function

Little baby: /* Function */

2014-06-05T02:53:16Z

‎Function

Little baby: /* Labs working on this gene */

2014-06-05T02:40:46Z

‎Labs working on this gene

← Older revision		Revision as of 05:01, 14 May 2015
Line 52:		Line 52:

	==Structured Information==		==Structured Information==
−	~~{{JaponicaGene\|~~	+	[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]
−	~~GeneName = Os01g0831900\|~~
−	~~Description = Similar to Ammonium transporter\|~~
−	~~Version = NM_001051237.1 GI:115440844 GeneID:4327434\|~~
−	~~Length = 1897 bp\|~~
−	~~Definition = Oryza sativa Japonica Group Os01g0831900, complete gene.\|~~
−	~~Source = Oryza sativa Japonica Group~~
−
−	~~ORGANISM Oryza sativa Japonica Group~~
−	~~Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;~~
−	~~Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP~~
−	~~clade; Ehrhartoideae; Oryzeae; Oryza.~~
−	\|
−	~~Chromosome = [[:category:Japonica Chromosome 1\|Chromosome 1]]\|~~
−	~~AP = Chromosome 1:37352969..37354865\|~~
−	~~CDS = 37353132..37353688,37353774..37354059,37354153..37354815\|~~
−	~~GCID = <gbrowseImage1>~~
−	~~name=NC_008394:37352969..37354865~~
−	~~source=RiceChromosome01~~
−	~~preset=GeneLocation~~
−	~~</gbrowseImage1>\|~~
−	~~GSID = <gbrowseImage2>~~
−	~~name=NC_008394:37352969..37354865~~
−	~~source=RiceChromosome01~~
−	~~preset=GeneLocation~~
−	~~</gbrowseImage2>\|~~
−	CDNA = <cdnaseq>atgcacctaaggatggcgtcgccgccgcagcccgggccgtacatgccggacctgccggcggtgccggcgtggctgaacaagggcgacaccgcgtggcagctggtggcggcgacgttcgtcggcatccagtcgatgcctgggctggtggtgatctacggcagcatcgtgaagaagaagtgggccgtcaactccgccttcatggcgctgtacgcctacgcgtccacgcttatcgtgtgggtgctcgtcggcttccgcatggcgttcggcgaccggctgctcccgttctgggccaaggccgggccggcgctgacgcaggacttcctggtgcaacgcgcggtgttcccggcgacggcgcactacggcagcgacggcacgctcgagacgccgcgcaccgagccgttctacgcggaggcggcgctggtgctgttcgagttcgagttcgcggccatcacgctggtgctgctcgccgggtcgctcctggggcggatgaacatcaaggcgtggatggcgttcaccccgctctggctcctcttctcctacaccgtcggcgcgttcagcctctggggcggcggcttcctctaccagtggggcgtcatcgactactccggcggatacgtcatccacctctcctccggcgtcgccggcttcaccgccgcctactgggtgggcccgaggctgaagagcgacagggagcggttctcgccgaacaacatcctgctcatgatcgccggcggcgggctgctgtggttgggctgggccgggttcaacggcggcgcgccgtacgcccccaacgtcaccgccacggtcgccgtgctcaacaccaacgtcagcgccgcgacgagcctcctcacctggacctgcctcgacgtcatcttcttcggcaagccctccgtcatcggcgccgtgcagggtatgatgacggggctcgtctgcatcacgcccggcgccgggctggtgcacacgtggtcagcgatgctgatgggcatgttcgccggcagcgtcccgtggttcacgatgatgatcctgcacaagaaatccaccttcctcatgaaggtcgacgacaccctcgccgtcttccacacccacgccgtcgccggcatcctgggcggcgtcctcacgggcctcctcgccacgccggagctctgcgctctcgattgcccgatcccgaacatgcgcggcgtcttctacggcagcggcatcggccagctcgggaagcagctcggcggcgcgctgttcgtcaccgtctggaacctcatcgtcaccagcgccattctcctctgcatcggcctcttcatcccgctccgcatgtccgacgaccagctcatgatcggcgacgacgcggcgcacggggaggaggcctacgctctgtggggggacggtgagaagttcgacgtgacgcggccggagacgacgaggacgggaggtgcaggcggcgcgggcagggaggacaccatggagcagaggctgaccaacatgggagccaggggtgtcaccattcagttgtag</cdnaseq>\|
−	AA = <aaseq>MHLRMASPPQPGPYMPDLPAVPAWLNKGDTAWQLVAATFVGIQS MPGLVVIYGSIVKKKWAVNSAFMALYAYASTLIVWVLVGFRMAFGDRLLPFWAKAGPA LTQDFLVQRAVFPATAHYGSDGTLETPRTEPFYAEAALVLFEFEFAAITLVLLAGSLL GRMNIKAWMAFTPLWLLFSYTVGAFSLWGGGFLYQWGVIDYSGGYVIHLSSGVAGFTA AYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPYAPNVTATVAVLNTNV SAATSLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHTWSAMLMGMFAGSV PWFTMMILHKKSTFLMKVDDTLAVFHTHAVAGILGGVLTGLLATPELCALDCPIPNMR GVFYGSGIGQLGKQLGGALFVTVWNLIVTSAILLCIGLFIPLRMSDDQLMIGDDAAHG EEAYALWGDGEKFDVTRPETTRTGGAGGAGREDTMEQRLTNMGARGVTIQL</aaseq>\|
−	DNA = <dnaseqindica>1178..1734#807..1092#51..713#acttcgcgttccatcgctcgctcgctctctcgctttgcttcgagctgagaatgcacctaaggatggcgtcgccgccgcagcccgggccgtacatgccggacctgccggcggtgccggcgtggctgaacaagggcgacaccgcgtggcagctggtggcggcgacgttcgtcggcatccagtcgatgcctgggctggtggtgatctacggcagcatcgtgaagaagaagtgggccgtcaactccgccttcatggcgctgtacgcctacgcgtccacgcttatcgtgtgggtgctcgtcggcttccgcatggcgttcggcgaccggctgctcccgttctgggccaaggccgggccggcgctgacgcaggacttcctggtgcaacgcgcggtgttcccggcgacggcgcactacggcagcgacggcacgctcgagacgccgcgcaccgagccgttctacgcggaggcggcgctggtgctgttcgagttcgagttcgcggccatcacgctggtgctgctcgccgggtcgctcctggggcggatgaacatcaaggcgtggatggcgttcaccccgctctggctcctcttctcctacaccgtcggcgcgttcagcctctggggcggcggcttcctctaccagtggggcgtcatcgactactccggcggatacgtcatccacctctcctccggcgtcgccggcttcaccgccgcctactgggtaaacacacgtgcaaccgtgcattgcatgcatcgtgttcgactgtttgttgcactattcacacgcctccatgttacaacgtgactctgacaggtgggcccgaggctgaagagcgacagggagcggttctcgccgaacaacatcctgctcatgatcgccggcggcgggctgctgtggttgggctgggccgggttcaacggcggcgcgccgtacgcccccaacgtcaccgccacggtcgccgtgctcaacaccaacgtcagcgccgcgacgagcctcctcacctggacctgcctcgacgtcatcttcttcggcaagccctccgtcatcggcgccgtgcagggtatgatgacggggctcgtctgcatcacgcccggcgccggtaacaaatctttcgtccgatcaaaaccacagcacacacacagttcatgggtccaaatcctcacgtacgttttgcatggacgcagggctggtgcacacgtggtcagcgatgctgatgggcatgttcgccggcagcgtcccgtggttcacgatgatgatcctgcacaagaaatccaccttcctcatgaaggtcgacgacaccctcgccgtcttccacacccacgccgtcgccggcatcctgggcggcgtcctcacgggcctcctcgccacgccggagctctgcgctctcgattgcccgatcccgaacatgcgcggcgtcttctacggcagcggcatcggccagctcgggaagcagctcggcggcgcgctgttcgtcaccgtctggaacctcatcgtcaccagcgccattctcctctgcatcggcctcttcatcccgctccgcatgtccgacgaccagctcatgatcggcgacgacgcggcgcacggggaggaggcctacgctctgtggggggacggtgagaagttcgacgtgacgcggccggagacgacgaggacgggaggtgcaggcggcgcgggcagggaggacaccatggagcagaggctgaccaacatgggagccaggggtgtcaccattcagttgtagaagacgggaagggtgtgcttgctcgttccagtgattctgatgatgtatggcactgatgtatgttactttcaacgtttagtgttaattcggtgatgtaatgctggtgtagtttctgttattgttaactgaattagttcatcgaaaacatgagaattatctggtt</dnaseqindica>\|
−	~~Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051237.1 RefSeq:Os01g0831900]\|~~
−	}}
−	[[Category:Genes]]
−	~~[[Category:Japonica mRNA]]~~
−	[[Category:Oryza Sativa Japonica Group]]
−	[[Category:Japonica ~~Genes]]~~
−	~~[[Category:Japonica Chromosome 1]]~~
−	~~[[Category:~~Chromosome 1]]

@@ Line 34: / Line 34: @@
 ==References==
-Please input cited references here.
 Xuefeng Ma， Zhijun Cheng， Ruizhen Qin et al. OsARG encodes an arginase that plays critical roles in panicle development and grain production in rice. ''The Plant Journal'' ，2013（ 73）：190–200

@@ Line 26: / Line 26: @@
 ==Labs working on this gene==
-Please input related labs here.
 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

@@ Line 20: / Line 20: @@
 ===Evolution===
-Please input evolution information here.
 You can also add sub-section(s) at will.

@@ Line 13: / Line 13: @@
 ===Expression===
-Please input expression information here.
 We examined the pattern of OsARG expression in various organs using quantitative real-time PCR. OsARG was expressed ubiquitously in all organs, including the root, stem, leaf blade, leaf sheath and panicle. Compared to WT, the expression in nglf-1 was reduced (Figure f). For a detailed evaluation of OsARG expression, we generated transgenic plants expressing the GUS reporter gene driven by the native promoter of OsARG. The GUS signal was found in the root, stem, leaf and panicle, with the strongest signal in the spikelets, consistent with the RT-PCR results (Figure g).

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-Please input function information here.
 Nitrogen is a crucial nutrient for plant growth and development. Arginine is considered to be an important amino acid for nitrogen transport and storage, playing a crucial role during plant seedling development. However, little is known about the role of arginine in nitrogen remobilization at the reproductive stage. We isolated a rice mutant nglf-1 with reduced plant height, small panicle and grain size, and low seed-setting rate (10% in nglf-1 compared to 93% in wild-type). Map-based cloning revealed that the mutant phenotype was caused by loss of function of a gene (OsARG) encoding an arginine hydrolysis enzyme, which is consistent with arginine accumulation in the mutant. The phenotype was partially corrected supplying exogenous nitrogen, and fully corrected by expression of a wild-type OsARG transgene. Over-expression of OsARG in rice (cv. Kitaake) increased grain number per plant under nitrogen-limited conditions. OsARG was ubiquitously expressed in various organs, but most strongly in developing panicles. The OsARG protein was localized in the mitochondria, consistent with other arginases. Our results suggest that the arginase encoded by OsARG, a key enzyme in Arg catabolism, plays a critical role during panicle development, especially under conditions of insufficient exogenous nitrogen. OsARG is a potential target for crop improvement.
 Crossing nglf-1 with IRAT129, a japonica variety, to construct a genetically segregating population. Within the F2 population, nglf-1 exhibited normal Mendelian segregation (1632 normal:560 nglf-1; v2 = 0.322; P > 0.05), indicating the involvement of a single gene mutation. Bulked segregant analysis with SSR markers covering the whole rice genome found that the RM335 marker on the short arm of chromosome 4 was linked to the nglf-1 locus, which was subsequently delimited using SSR primers AL36-1 and AL1-1, and further mapped to a 72 kb interval, delimited by the two markers In10 and AL1-1, using 510 F2 mutant plants (Figure a). Based on the Rice Genome Annotation Project (http://rice.plantbiology.msu.edu/), the mapped region contains 13 recognizable open reading frames (ORFs). Sequence comparison revealed a single nucleotide substitution resulting in a stop codon (TGA) in ORF8 of nglf-1. ORF8, gene LOC_Os04g01590, encodes a protein homologous to arginase and consists of six exons, with a total size of 1382 bp, including a 1023 bp coding region, a 126 bp 5′ UTR, and a 233 bp 3′ UTR. Thus the substitution occurred in the arginase domain of nglf-1, resulting in premature termination of the protein (Figure b), and thus ORF8 was considered a candidate for putative gene Nglf.

@@ Line 15: / Line 15: @@
 Please input expression information here.
 We examined the pattern of OsARG expression in various organs using quantitative real-time PCR. OsARG was expressed ubiquitously in all organs, including the root, stem, leaf blade, leaf sheath and panicle. Compared to WT, the expression in nglf-1 was reduced (Figure f). For a detailed evaluation of OsARG expression, we generated transgenic plants expressing the GUS reporter gene driven by the native promoter of OsARG. The GUS signal was found in the root, stem, leaf and panicle, with the strongest signal in the spikelets, consistent with the RT-PCR results (Figure g).
 [[File:]]
-[[File:]]
+[[File:little baby f.png]]
 ===Evolution===

@@ Line 9: / Line 9: @@
 In the rice genome, OsARG is the only gene encoding arginase. A BLAST search with translated OsARG revealed that most plant arginases are encoded by one or two genes.OsARG has high identity with proteins from other organisms, including Arabidopsis thaliana (Flores et al., 2008), Solanum lycopersicum (Chen et al., 2004), Pinus taeda (Todd et al., 2001) and Glycine max (Goldraij and Polacco, 1999) . OsARG also has high identity with arginases from five genera of monocotyledonous plants, including the arginases in Zea mays, Sorghum bicolor, Brachypodium distachyon, Triticum aestivum and Hordeum vulgare, all of which are members of the same clade, distinguishing them from all dicotyledonous species analyzed. The shared identity may indicate a different role in monocotyledonous plants than in dicotyledonous species . The OsARG protein comprises 340 residues with a predicted molecular mass of 36.96 kDa and pI of 5.90. Sequencealignment indicated a conserved arginase domain, and all plant arginases contain two His and four Asp residues that bind the Mn2+ co-factor (Chen et al., 2004). A predicted mitochondrial targeting peptide is located at the N-terminal end, and this region showed the greatest diversity among various plant arginases
 Improving nitrogen use efficiency is an important objective of breeding programs. In maize over-expressing the Gln1-3 gene, an increase in kernel number was observed under either high-nitrogen or low-nitrogen growth conditions (Martin et al., 2006). A recent investigation of rice transformed with the cytosolic glutamine synthetase (GS1) gene indicated that, due to enhanced nitrogen use efficiency, lines over-expressing GS1 exhibited a 25–35% higher spikelet yield (Brauer et al., 2011). Our data indicate the possibility of increasing OsARG expression, highlighting the potential use of manipulation of OsARG expression to improve rice yield under sub-optimal nitrogen conditions. However, extensive field experiments are needed. OsARG not only functions in nitrogen catabolism, but also participates directly in the control of grain yield in rice.
 [[File:little baby a.b.png]][[File:little baby c.d.e.png]]