Os01g0859300 - Revision history

Xysj1980: /* Mutation */

2017-03-27T12:08:03Z

‎Mutation

Xysj2012: /* Structured Information */

2015-05-13T07:41:08Z

‎Structured Information

Zhangye: /* Knowledge Extension */

2014-12-26T15:21:00Z

‎Knowledge Extension

Zhangye at 11:41, 24 December 2014

2014-12-24T11:41:54Z

Zhangye: /* Function */

2014-12-24T04:06:54Z

‎Function

Zhangye: /* Knowledge Extension */

2014-12-24T01:37:33Z

‎Knowledge Extension

Zhangye: /* Evolution */

2014-12-24T01:32:44Z

‎Evolution

Zhangye: /* Subcellular localization */

2014-12-24T01:29:55Z

‎Subcellular localization

Zhangye: /* Expression */

2014-12-24T01:27:50Z

‎Expression

Zhangye: /* Mutation */

2014-12-24T01:14:16Z

‎Mutation

@@ Line 14: / Line 14: @@
 ''OsABI5-1'' and ''OsABI5-2'' had obvious transactivation capacity.By quantitative b-galactosidase assays using ONPG as a substrate, the ''OsABI5-1'' fusion construct showed a nearly twofold higher activity level than the ''OsABI5-2'' fusion construct, suggesting that deletion of additional protein sequences elevated the transactivation activity, and the additional 10 amino acids behind the bZIP domain of ''OsABI5-2'' can affect the activity strength<ref name="ref2" />.
 *DNA binding activity of ''OsABI5-1'' and ''OsABI5-2'' to the cis-acting G-box element in yeast:
-''OsABI5-2'' can function as a transacting factor for the G-box element and the additional 10 amino acids of ''OsABI5-2'' are necessary for the binding specificity to the G-box. The binding activity of O''sABI5-1'' to the G-box element is disrupted by deleting the 10 amino acids<ref name="ref2" />.
+* ''OsABI5-2'' can function as a transacting factor for the G-box element and the additional 10 amino acids of ''OsABI5-2'' are necessary for the binding specificity to the G-box. The binding activity of O''sABI5-1'' to the G-box element is disrupted by deleting the 10 amino acids<ref name="ref2" />.
 *Interaction between ''OsABI5-1'' and ''OsABI5-2'':
 ''OsABI5-1'' and ''OsABI5-2'' could interact directly in vitro, and that ''OsABI5-2'' could interact with itself. In Arabidopsis, hetero and homo-dimerization among homologs of the ''ABI5'' family have already been documented. Whether ''OsABI5-1'' and ''OsABI5-2'' could form heterodimers remains to be confirmed<ref name="ref2" />.

@@ Line 62: / Line 62: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 35: / Line 35: @@
 ===Knowledge Extension===
-*Abscisic Acid (ABA) is an important phytohormone involved in abiotic stress resistance in plants. A group of bZIP transcription factors play important roles in the ABA signaling pathway in ''Arabidopsis''. ''OsABI5''(''Os01g0859300'') is a number of bZIP transcription factors,which also named ''OsbZIP10''(''Os01g0859300''). Most of these ''OsbZIPs'' were significantly induced by ABA, ACC and abiotic stresses<ref name="ref5"/>.
+*Abscisic Acid (ABA) is an important phytohormone involved in abiotic stress resistance in plants. A group of bZIP transcription factors play important roles in the ABA signaling pathway in ''Arabidopsis''. ''OsABI5''(''Os01g0859300'') is a member of bZIP transcription factors,which also named ''OsbZIP10''(''Os01g0859300''). Most of these ''OsbZIPs'' were significantly induced by ABA, ACC and abiotic stresses<ref name="ref5"/>.
 *All of the bZIPs could be classified into three subgroups. Each subgroup contains both rice and Arabidopsis bZIPs, indicating that the divergence of these subgroups predated the divergence of dicots and monocots. It also indicated that the protein sequences of these bZIPs were well conserved between dicots and monocots<ref name="ref5"/>.
 *Co-transformation experiments with ABI5 cDNA constructs resulted in specific transactivation of the ABA-inducible wheat Em, Arabidopsis AtEm6, bean-Phaseolin, and barley HVA1 and HVA22 promoters<ref name="ref4"/>.

@@ Line 59: / Line 59: @@
 Lu G, Gao C, Zheng X, et al. Identification of OsbZIP72 as a positive regulator of ABA response and drought tolerance in rice[J]. Planta, 2009, 229(3): 605-615.
 </ref>
-<references>
+</references>
 ==Structured Information==

@@ Line 5: / Line 5: @@
 Two ''OsABI5'' splicing variants were identified, designated ''OsABI5-1'', and ''OsABI5-2'' and their different expression patterns in tissues were analyzed. ''OsABI5'' variants may have overlapping and distinct functions to fine tune gene expression in ABA signaling as transcription factors together with ''OsVP1''
 <ref name="ref2"/>.
 ===Mutation===

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 ===Evolution===
-Please input evolution information here.
+*homology gene:
+**It shares high amino acid sequence homology with Arabidopsis ''AtABI5''and barley ''HvABI5'' within five regions. Amino acids homology analysis showed that the OsABI5-encoded peptide contains a typical basic leucine zipper domain. The deduced amino acid sequence of ''OsABI5'' contains conserved regions similar to other ''ABI5'' or ''ABI5-like'' genes in other species. The Arabidopsis ''AtABI5'' gene is involved in seed dormancy, maturation and stress responses. <ref name="ref1"/><ref name="ref3"/>.
 ===Knowledge Extension===

@@ Line 25: / Line 25: @@
 ===Subcellular localization===
+The OsABI5::GFP fusion protein was targeted to the nuclei of the cells, which suggested that OsABI5 was a nuclear protein and functioned as a transcription factor to regulate expression of down-stream genes<ref name="ref1" />.
 ===Evolution===

@@ Line 17: / Line 17: @@
 ===Expression===
-Please input expression information here.
+[[File: OsABI5 ExpressionPattern1.png|right|thumb|300px|'''Figure 1.''' ''RT-PCR analysis of OsABI5 expression patterns under ABA and stress conditions(from reference <ref name="ref1"/>).'']]
 ===Subcellular localization===

← Older revision		Revision as of 01:14, 24 December 2014
Line 7:		Line 7:

	===Mutation===		===Mutation===
		+	''OsABI5-1'' and ''OsABI5-2''<br>
		+	*Sequence analysis showed that the two ''OsABI5'' cDNAs are transcribed from the same initiation site and have a different exon. Exon skip was presented in the splicing variants. Amino acid analysis showed that the encoded peptides shared the identical bZIP domain, and the additional 10 amino acids of ''OsABI5-2'' presented behind the leucine zipper at the C-terminal. Southern blot analysis indicated that the ''OsABI5'' gene presented only one copy in the rice genome, which provide further support that ''OsABI5-1'' and ''OsABI5-2'' are different splicing variants of the ''OsABI5'' gene<ref name="ref2" />.
		+	*Differential transactivation activity in ''OsABI5-1'' and ''OsABI5-2'':
		+	''OsABI5-1'' and ''OsABI5-2'' had obvious transactivation capacity.By quantitative b-galactosidase assays using ONPG as a substrate, the ''OsABI5-1'' fusion construct showed a nearly twofold higher activity level than the ''OsABI5-2'' fusion construct, suggesting that deletion of additional protein sequences elevated the transactivation activity, and the additional 10 amino acids behind the bZIP domain of ''OsABI5-2'' can affect the activity strength<ref name="ref2" />.
		+	*DNA binding activity of ''OsABI5-1'' and ''OsABI5-2'' to the cis-acting G-box element in yeast:
		+	''OsABI5-2'' can function as a transacting factor for the G-box element and the additional 10 amino acids of ''OsABI5-2'' are necessary for the binding specificity to the G-box. The binding activity of O''sABI5-1'' to the G-box element is disrupted by deleting the 10 amino acids<ref name="ref2" />.
		+	*Interaction between ''OsABI5-1'' and ''OsABI5-2'':
		+	''OsABI5-1'' and ''OsABI5-2'' could interact directly in vitro, and that ''OsABI5-2'' could interact with itself. In Arabidopsis, hetero and homo-dimerization among homologs of the ''ABI5'' family have already been documented. Whether ''OsABI5-1'' and ''OsABI5-2'' could form heterodimers remains to be confirmed<ref name="ref2" />.

	===Expression===		===Expression===