Os01g0869200 - Revision history

Xysj2017 at 09:46, 14 February 2017

2017-02-14T09:46:35Z

Xysj2017: /* Annotated Information */

2017-02-14T09:44:12Z

‎Annotated Information

Xysj2017 at 09:44, 14 February 2017

2017-02-14T09:44:04Z

Xysj2017 at 09:42, 14 February 2017

2017-02-14T09:42:41Z

Xysj2017: /* Sequence Analysis of OsMGT1 in Rice */

2017-02-14T09:41:44Z

‎Sequence Analysis of OsMGT1 in Rice

Xysj2017 at 09:41, 14 February 2017

2017-02-14T09:41:12Z

192.168.72.52 at 05:03, 14 May 2015

2015-05-14T05:03:26Z

Tuntun: /* Expression */

2014-06-09T12:32:07Z

‎Expression

Tuntun: /* Expression */

2014-06-09T12:29:41Z

‎Expression

Tuntun at 12:17, 9 June 2014

2014-06-09T12:17:30Z

@@ Line 17: / Line 17: @@
 * However, in art1 (a mutant defective in the Al response C2H2-type zinc-finger transcription factor), the Al-induced expression was not observed , confirming that OsMGT1 expression was regulated by ART1.
-===Sequence Analysis of OsMGT1 in Rice===
+===Sequence Analysis ===
 * OsMGT1 (Os01g0869200) cloned from rice (cv Nipponbare) contains six exons and five introns, encoding a peptide of 418 amino acids.
 *  Using the SOSUI program (http://bp.nuap.nagoya-u.ac.jp/sosui/), it has been predicted that OsMGT1 is a membrane protein with two transmembrane domains near the C terminus.
-* OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane  (domain.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/bin/PP_199778_LW_f1.gif)
+* OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane.
-===Evolution===
+===Sub-cellular Localization of OsMGT1===
 ==Labs working on this gene==

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:Example1234567.jpg|right|thumb|427px|'''Figure 1.''' ''Expression pattern of OsMGT1 in rice.<ref name="ref1" />.'']]
+[[File:Example1234567.jpg|right|thumb|327px|'''Figure 1.''' ''Expression pattern of OsMGT1 in rice.<ref name="ref1" />.'']]
 ===Function===
 * Up-Regulation of a Magnesium Transporter Gene '''''OsMGT1''''' Is Required for Conferring Aluminum Tolerance in Rice.
@@ Line 32: / Line 32: @@
 ===Evolution===
-−
 ==Labs working on this gene==
 Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan (Z.C.C., N.Y., J.F.M.); and Genome Resource Center, Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8602, Japan (R.M., Y.N.)

@@ Line 2: / Line 2: @@
 ==Annotated Information==
 ===Function===
 * Up-Regulation of a Magnesium Transporter Gene '''''OsMGT1''''' Is Required for Conferring Aluminum Tolerance in Rice.
@@ Line 20: / Line 21: @@
 * OsMGT1 (Os01g0869200) cloned from rice (cv Nipponbare) contains six exons and five introns, encoding a peptide of 418 amino acids.
 *  Using the SOSUI program (http://bp.nuap.nagoya-u.ac.jp/sosui/), it has been predicted that OsMGT1 is a membrane protein with two transmembrane domains near the C terminus.
-* OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane  (domain.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/bin/PP_199778_LW_f1.gif) [[File:Example1234567.jpg]]
+* OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane  (domain.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/bin/PP_199778_LW_f1.gif)
 Sub-cellular Localization of OsMGT1

@@ Line 11: / Line 11: @@
 ===Expression===
+* Real-time reverse transcription (RT)-PCR analysis revealed that OsMGT1 was expressed in both the shoots and roots at similar level in the absence of Al.
 ===Sequence Analysis of OsMGT1 in Rice===
@@ Line 21: / Line 24: @@
 Expression Pattern of OsMGT1
-Real-time reverse transcription (RT)-PCR analysis revealed that OsMGT1 was expressed in both the shoots and roots at similar level in the absence of Al . However, in the presence of Al, the expression in the roots was up-regulated by 5 times, but that in the shoots was unaffected. Root spatial analysis showed that OsMGT1 was expressed in both the root tips (0–1 cm) and mature region (1–2 cm) and the expression in both regions were up-regulated by Al. However, in art1 (a mutant defective in the Al response C2H2-type zinc-finger transcription factor), the Al-induced expression was not observed , confirming that OsMGT1 expression was regulated by ART1.
 [[File:Example123456.jpg]]

@@ Line 21: / Line 21: @@
 Expression Pattern of OsMGT1
-Real-time reverse transcription (RT)-PCR analysis revealed that OsMGT1 was expressed in both the shoots and roots at similar level in the absence of Al . However, in the presence of Al, the expression in the roots was up-regulated by 5 times, but that in the shoots was unaffected. Root spatial analysis showed that OsMGT1 was expressed in both the root tips (0–1 cm) and mature region (1–2 cm) and the expression in both regions were up-regulated by Al. However, in art1 (a mutant defective in the Al response C2H2-type zinc-finger transcription factor), the Al-induced expression was not observed , confirming that OsMGT1 expression was regulated by ART1.(http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/figure/fig2/)[[File:Example123456.jpg]]
+Real-time reverse transcription (RT)-PCR analysis revealed that OsMGT1 was expressed in both the shoots and roots at similar level in the absence of Al . However, in the presence of Al, the expression in the roots was up-regulated by 5 times, but that in the shoots was unaffected. Root spatial analysis showed that OsMGT1 was expressed in both the root tips (0–1 cm) and mature region (1–2 cm) and the expression in both regions were up-regulated by Al. However, in art1 (a mutant defective in the Al response C2H2-type zinc-finger transcription factor), the Al-induced expression was not observed , confirming that OsMGT1 expression was regulated by ART1.
 Sub-cellular Localization of OsMGT1

@@ Line 30: / Line 30: @@
    Plant Physiology, 2012, 159(4): 1624-1633
 ==Structured Information==
-{{JaponicaGene|
+     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]

@@ Line 10: / Line 10: @@
 Sequence Analysis of OsMGT1 in Rice
-OsMGT1 (Os01g0869200) cloned from rice (cv Nipponbare) contains six exons and five introns (Supplemental Fig. S1A), encoding a peptide of 418 amino acids. Using the SOSUI program (http://bp.nuap.nagoya-u.ac.jp/sosui/), it has been predicted that OsMGT1 is a membrane protein with two transmembrane domains near the C terminus (Supplemental Fig. S1B). OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane  (domain.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/bin/PP_199778_LW_f1.gif)
+OsMGT1 (Os01g0869200) cloned from rice (cv Nipponbare) contains six exons and five introns (Supplemental Fig. S1A), encoding a peptide of 418 amino acids. Using the SOSUI program (http://bp.nuap.nagoya-u.ac.jp/sosui/), it has been predicted that OsMGT1 is a membrane protein with two transmembrane domains near the C terminus (Supplemental Fig. S1B). OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane  (domain.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/bin/PP_199778_LW_f1.gif) [[File:Example1234567.jpg]]
 Expression Pattern of OsMGT1
@@ Line 19: / Line 19: @@
 The subcellular localization of OsMGT1 was investigated in rice protoplasts by transiently expressing OsMGT1 fused with GFP at both C terminal and N terminal under the control of 35S promoter. Either construct gave a signal at the plasma membrane , in contrast to GFP alone, which signal was observed in cytosol and nuclei.(http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/figure/fig3/)
 ===Evolution===

@@ Line 6: / Line 6: @@
 ===Expression===
-     OsMGT1 was expressed in both the roots and shoots in the absence of Al, but the expression only in the roots was rapidly up-regulated by Al. Furthermore, the expression did not respond to low pH and other metals including cadmium and lanthanum, and was regulated by an Al-responsive transcription factor, AL RESISTANCE TRANSCRIPTION FACTOR1. An investigation of subcellular localization showed that OsMGT1 was localized to the plasma membrane.
-===Evolution===
+Sequence Analysis of OsMGT1 in Rice
-Please input evolution information here.
 ==Labs working on this gene==
-Please input related labs here.
+Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan (Z.C.C., N.Y., J.F.M.); and Genome Resource Center, Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8602, Japan (R.M., Y.N.)
 ==References==
-Please input cited references here.
+. Zhi Chang Chen;Naoki Yamaji;Ritsuko Motoyama;Yoshiaki Nagamura;Jian Feng Ma
+  Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice
 ==Structured Information==
 {{JaponicaGene|