Os01g0907900 - Revision history

Zhuhuihui: /* Expression */

2014-06-02T09:56:25Z

‎Expression

Zhuhuihui: /* Expression */

2014-06-02T09:55:10Z

‎Expression

Zhuhuihui: /* Expression */

2014-06-02T09:53:09Z

‎Expression

Zhuhuihui: /* Phenotypes of pla2 Mutants */

2014-06-02T09:47:07Z

‎Phenotypes of pla2 Mutants

Zhuhuihui: /* Expression */

2014-06-02T09:43:30Z

‎Expression

Zhuhuihui: /* Expression */

2014-06-02T09:43:04Z

‎Expression

Zhuhuihui: /* Evolution */

2014-06-02T09:40:38Z

‎Evolution

Zhuhuihui: /* Evolution */

2014-06-02T09:39:37Z

‎Evolution

Zhuhuihui: /* Evolution */

2014-06-02T09:36:13Z

‎Evolution

Zhuhuihui: /* Evolution */

2014-06-02T08:50:27Z

‎Evolution

@@ Line 25: / Line 25: @@
 expressed in bracts and several external layers of the rachis meristem (Figure 6G), suggesting that at this stage, PLA2 may regulate meristem identity directly. At the later stages, PLA2 was expressed in branch meristems, floral meristems, and floral organs (Figures 6H and 6I). In the control experiment hybridized with sense RNA probe, no hybridization signals were detected(Figure 6J).<ref name="ref1" />.
-[[File:Figure6.jpg "In Situ Localization of PLA2 Transcripts"]]
+[[File:Figure6.jpg ]]
 (A) to (I) Antisense probe.
 (J) Sense probe.

@@ Line 25: / Line 25: @@
 expressed in bracts and several external layers of the rachis meristem (Figure 6G), suggesting that at this stage, PLA2 may regulate meristem identity directly. At the later stages, PLA2 was expressed in branch meristems, floral meristems, and floral organs (Figures 6H and 6I). In the control experiment hybridized with sense RNA probe, no hybridization signals were detected(Figure 6J).<ref name="ref1" />.
-[[File:Figure6.jpg"In Situ Localization of PLA2 Transcripts"]]
+[[File:Figure6.jpg "In Situ Localization of PLA2 Transcripts"]]
 (A) to (I) Antisense probe.
 (J) Sense probe.

@@ Line 25: / Line 25: @@
 expressed in bracts and several external layers of the rachis meristem (Figure 6G), suggesting that at this stage, PLA2 may regulate meristem identity directly. At the later stages, PLA2 was expressed in branch meristems, floral meristems, and floral organs (Figures 6H and 6I). In the control experiment hybridized with sense RNA probe, no hybridization signals were detected(Figure 6J).<ref name="ref1" />.
-[[File:Figure6.jpg]]In Situ Localization of PLA2 Transcripts.
+[[File:Figure6.jpg"In Situ Localization of PLA2 Transcripts"]]
 (A) to (I) Antisense probe.
 (J) Sense probe.
 (A) Longitudinal section of embryo at 10 d after pollination. sm, SAM; vb, vascular bundle; ra, radicle.
 (B) Crown root at 1 week after germination.
 (C) Longitudinal section of 3-week-old shoot apex.
 (D) to (F) Serial transverse sections of 3-week-old shoot apex. Panels are from the bottom to the top. PLA2 signals are strong in marginal regions.
 (G) Inflorescence apex at stage In2. Two primary rachis branches (asterisks) are formed. b1 and b2 indicate first and second bracts, respectively.
 (H) Inflorescence apex at stage In5. Asterisks indicate spikelet primordia.

@@ Line 55: / Line 55: @@
 shorter than pla1 (1.8 versus 2.3). Furthermore, in contrast with pla1, in which the angle between the blade and sheath is increased for all leaves, the third leaf of pla2 appears erect due to an irregular blade-sheath boundary (see Supplemental Figure 1 online). The transition of vegetative to reproductive phase was also delayed in pla2-1 and pla2-2. Together with an extended vegetative period, pla2 produced threefold as many leaves as the wild type (49 versus 16 leaves). Assuming that this increase reflects loss of normal PLA2 function, these results suggest
 that the PLA2 gene normally acts to inhibit leaf initiation. In addition to a shortened plastochron, pla2 plants exhibited significantly
-smaller leaves than the wild type with respect to the length of blade and sheath and also the width of leaf blade(Figure 1B; see Supplemental Figures 2A to 2C online). As the size of epidermal cells of the third leaf in pla2 was almost comparable to that in the wild type, this size reduction was exclusively due to the reduction in the number of cells (see Supplemental Figure 3 online). The possibility of a causal relationship between leaf size and plastochron is reinforced by the phenotype of pla1, which also shows reduced leaf size and plastochron but not as severe as seen for pla2 (Figure 1B; see Supplemental Figures 2A to 2C online). Thus, there exists a positive correlation between plastochron and leaf size in the wild type, pla1, and pla2.[[File:Figure1.0.jpg]]
+smaller leaves than the wild type with respect to the length of blade and sheath and also the width of leaf blade(Figure 1B; see Supplemental Figures 2A to 2C online). As the size of epidermal cells of the third leaf in pla2 was almost comparable to that in the wild type, this size reduction was exclusively due to the reduction in the number of cells . The possibility of a causal relationship between leaf size and plastochron is reinforced by the phenotype of pla1, which also shows reduced leaf size and plastochron but not as severe as seen for pla2 (Figure 1B; see Supplemental Figures 2A to 2C online). Thus, there exists a positive correlation between plastochron and leaf size in the wild type, pla1, and pla2.[[File:Figure1.0.jpg]][[File:Figure2222.jpg]]
 ===Evolution===

@@ Line 25: / Line 25: @@
 expressed in bracts and several external layers of the rachis meristem (Figure 6G), suggesting that at this stage, PLA2 may regulate meristem identity directly. At the later stages, PLA2 was expressed in branch meristems, floral meristems, and floral organs (Figures 6H and 6I). In the control experiment hybridized with sense RNA probe, no hybridization signals were detected(Figure 6J).<ref name="ref1" />.
-[[File:Figure6.jpg]In Situ Localization of PLA2 Transcripts.
+[[File:Figure6.jpg]]In Situ Localization of PLA2 Transcripts.
 (A) to (I) Antisense probe.

@@ Line 39: / Line 39: @@
 The predicted PLA2 protein contains three RNA recognition motifs (RRM1, RRM2, and RRM3) and shows high similarity to the maize TE1 and the fission yeast MEI2 proteins (Figures 5B and 5C). The amino acid identities between PLA2 and TE1 in the three RRMs were 75.4, 74.6, and 89.5%, respectively, and those between PLA2 and MEI2 were 24.6, 29.9, and 52.6%, respectively (Figures 5B and 5C).
 Anderson et al. (2004) have reported six MEI2-like proteins in rice and nine in Arabidopsis and divided them into two subfamilies: a MEI2-like subfamily and a TE1-like subfamily. PLA2 is identical to OML1 of the TE1-like subfamily. By BLAST search, we have identified an additional MEI2-like protein in rice, OML7, which like OML6, has only the C-terminal RRM. A phylogenetic tree based on a comparison of the highly conserved RRM3 domain indicates that PLA2 is likely to be the orthologue of TE1 (Figure 5D; see Supplemental Figure 5 online).[[File:Figure5.0.jpg]]
-Structures of PLA2 and Related Genes.
+Structures of PLA2 and Related Genes.<ref name="ref1" />.
 (A) Exon/intron structure of the PLA2 gene. Six boxes indicate exons. RNA recognition motifs (RRMs) are shaded. Locations of the two pla2 mutations are indicated: base and amino acid substitutions in exon 6 in pla2-1 and base substitution and non-sense mutation in pla2-2.
 (B) Deduced amino acid sequence of PLA2 protein. Underlining indicates RRMs.

@@ Line 38: / Line 38: @@
 ), terminal ear1 (te1) in maize (Zea mays; Veit et al., 1998), and ALTEREDMERISTEM PROGRAM1 (AMP1), PHYTOCHROME B (PHYB), and SERRATE (SE) in Arabidopsis thaliana (Reed et al., 1993;Helliwell et al., 2001; Prigge andWagner, 2001). pla1, te1, and amp1 show shorter plastochron. By contrast, phyB and se show longer plastochron than the wild type. With each of these genes encoding a distinct class of protein and showing distinct loss-offunction phenotypes, the regulation of plastochron appears complex.
 The predicted PLA2 protein contains three RNA recognition motifs (RRM1, RRM2, and RRM3) and shows high similarity to the maize TE1 and the fission yeast MEI2 proteins (Figures 5B and 5C). The amino acid identities between PLA2 and TE1 in the three RRMs were 75.4, 74.6, and 89.5%, respectively, and those between PLA2 and MEI2 were 24.6, 29.9, and 52.6%, respectively (Figures 5B and 5C).
-Anderson et al. (2004) have reported six MEI2-like proteins in rice and nine in Arabidopsis and divided them into two subfamilies: a MEI2-like subfamily and a TE1-like subfamily. PLA2 is identical to OML1 of the TE1-like subfamily. By BLAST search, we have identified an additional MEI2-like protein in rice, OML7, which like OML6, has only the C-terminal RRM. A phylogenetic tree based on a comparison of the highly conserved RRM3 domain indicates that PLA2 is likely to be the orthologue of TE1 (Figure 5D; see Supplemental Figure 5 online).[[File:Figure5.0jpg|right|thumb|150px|]]
+Anderson et al. (2004) have reported six MEI2-like proteins in rice and nine in Arabidopsis and divided them into two subfamilies: a MEI2-like subfamily and a TE1-like subfamily. PLA2 is identical to OML1 of the TE1-like subfamily. By BLAST search, we have identified an additional MEI2-like protein in rice, OML7, which like OML6, has only the C-terminal RRM. A phylogenetic tree based on a comparison of the highly conserved RRM3 domain indicates that PLA2 is likely to be the orthologue of TE1 (Figure 5D; see Supplemental Figure 5 online).[[File:Figure5.0.jpg]]
 ==Labs working on this gene==