Os01g0927000 - Revision history

Guangyi: /* References */

2016-10-10T06:26:26Z

‎References

Guangyi: /* References */

2016-10-10T06:25:52Z

‎References

Guangyi at 06:25, 10 October 2016

2016-10-10T06:25:23Z

Guangyi: /* References */

2016-10-10T06:23:07Z

‎References

Guangyi: /* References */

2016-10-10T06:20:32Z

‎References

Guangyi: /* Annotated Information */

2016-10-10T06:18:59Z

‎Annotated Information

Guangyi: /* Annotated Information */

2016-10-10T06:18:15Z

‎Annotated Information

Guangyi: /* Annotated Information */

2016-10-10T06:17:19Z

‎Annotated Information

Guangyi: /* Annotated Information */

2016-10-10T06:15:16Z

‎Annotated Information

Guangyi: /* References */

2016-10-10T06:12:30Z

‎References

@@ Line 33: / Line 33: @@
 </references>
 <br>
 <br>

@@ Line 33: / Line 33: @@
 </references>
 ==Structured Information==
       [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]

@@ Line 1: / Line 1: @@
-*The rice '''Os01g0927000''' was reported as '''SDG714''' in 2007 [1] by researchers from the USA.
+*The rice '''Os01g0927000''' was reported as '''SDG714''' in 2007 <ref name="ref1" /> by researchers from the USA.
 <br>
 ==Annotated Information==

@@ Line 29: / Line 29: @@
 <references>
 <ref name="ref1">
-Adai A, Johnson C, Mlotshwa S, Archer-Evans S, Manocha V, Vance V,Sundaresan V (2005) Computational prediction of miRNAs in Arabidopsis thaliana. Genome Res 15: 78–91
+Alvarez-Venegas, R., Pien, S., Sadder, M., Witmer, X., Grossniklaus,U., and Avramova, Z. (2003). ATX-1, an Arabidopsis homolog of trithorax, activates flower homeotic genes. Curr. Biol. 13: 627–637.
 </ref>

@@ Line 29: / Line 29: @@
 <references>
 <ref name="ref1">
-Alvarez-Venegas, R., Pien, S., Sadder, M., Witmer, X., Grossniklaus,U., and Avramova, Z. (2003). ATX-1, an Arabidopsis homolog of trithorax, activates flower homeotic genes. Curr. Biol. 13: 627–637.
+Adai A, Johnson C, Mlotshwa S, Archer-Evans S, Manocha V, Vance V,Sundaresan V (2005) Computational prediction of miRNAs in Arabidopsis thaliana. Genome Res 15: 78–91
 </ref>

@@ Line 2: / Line 2: @@
 <br>
 ==Annotated Information==
-[[File:24-f1.png |center |thumb |10000px |''''''Figure 2. Methyltransferase Activity and Site Specificity of SDG714 in Vitro.(A) Methyltransferase activity of SDG714. GST and GST-SDG714 fusion proteins were tested for their ability to methylate oligonucleosomes or core histones. Enzymes and substrates used are indicated at top. The left panel shows the Coomassie blue–stained gel. The positions of GST and GSTSDG714 fusion proteins and different histones are indicated at right. Autoradiography in the right panel indicates methyltransferase activity and specificity.(B) and (C) Site specificity of SDG714 (B) and the C-terminal part with pre-SET, SET, and post-SET domains of SDG714 (SDG714C; 319 to 663 amino acids) (C). Methyltransferase assays were performed using substrates, as GST-NH3 contains the N-terminal 1 to 57 amino acids of histone H3 fused with GST and point mutations at histone H3K4, H3K9, or H3K27 to Arg (R), named GST-H3N1-57R4, GST-H3N1-57R9, and GST-H3N1-57R27 respectively. Substrates used are indicated at top. The top panels show Coomassie blue–stained gels, and the positions of GST and GST-SDG714C
+[[File:24-f1.png |right |thumb |10000px |''''''Figure 2. Methyltransferase Activity and Site Specificity of SDG714 in Vitro.(A) Methyltransferase activity of SDG714. GST and GST-SDG714 fusion proteins were tested for their ability to methylate oligonucleosomes or core histones. Enzymes and substrates used are indicated at top. The left panel shows the Coomassie blue–stained gel. The positions of GST and GSTSDG714 fusion proteins and different histones are indicated at right. Autoradiography in the right panel indicates methyltransferase activity and specificity.(B) and (C) Site specificity of SDG714 (B) and the C-terminal part with pre-SET, SET, and post-SET domains of SDG714 (SDG714C; 319 to 663 amino acids) (C). Methyltransferase assays were performed using substrates, as GST-NH3 contains the N-terminal 1 to 57 amino acids of histone H3 fused with GST and point mutations at histone H3K4, H3K9, or H3K27 to Arg (R), named GST-H3N1-57R4, GST-H3N1-57R9, and GST-H3N1-57R27 respectively. Substrates used are indicated at top. The top panels show Coomassie blue–stained gels, and the positions of GST and GST-SDG714C
 fusion proteins are indicated at right. Autoradiography in the bottom panels indicates methyltransferase activity and specificity'''''']]
 <br>

@@ Line 11: / Line 11: @@
 <br>
-===Expression===
+===Mutation===
-Please input expression information here.
+*Loss-of-function mutants of SDG714 (SDG714IR transformants) generated by RNA interference display a mostly glabrous phenotype as a result of the lack of macro trichomes in glumes, leaves, and culms compared with control plants. These mutants also show decreased levels of CpG and CNG cytosine methylation as well as H3K9 methylation at the Tos17 locus, a copia-like retrotransposon widely used for the generation of rice mutants. Most interestingly, loss of function of SDG714 can enhance transcription and cause the transposition of Tos17.
+<br>
 ==Labs working on this gene==