Os01g44260 - Revision history

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2014-06-09T13:48:04Z

‎References

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‎Structured Information

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‎Function

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‎Stress responsive changes in expression of genes of the flavonoid pathway

Active: /* Anthocyanin accumulation in response to stress is genotype dependent */

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‎Anthocyanin accumulation in response to stress is genotype dependent

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‎Introduction

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‎References

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‎References

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 ==References==
 <references>
-<ref name="ref1">Nagabhushana Ithal, Arjula R. Reddy. (2004) Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB. Plant Science 166(6): 1505-1513</ref>
+<ref name="ref1">Nagabhushana Ithal, Arjula R. Reddy. (2004) Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB. Plant Science 166(6): 1505-1513.</ref>
 <ref name="ref2">[[http://rice.plantbiology.msu.edu/cgi-bin/ORF_infopage.cgi?orf=LOC_Os01g44260]]</ref>
 </references>

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 ==Structured Information==
-===Gene Identification ===
+===Gene Identification<ref name="ref2"/> ===
 Gene Product Name:	dihydroflavonol-4-reductase, putative, expressed
 Locus Name:	LOC_Os01g44260.1
-===Gene Attributes ===
+===Gene Attributes<ref name="ref2"/> ===
 Chromosome:	                Chr1
@@ Line 92: / Line 92: @@
 Predicted pI:	                4.8564
-=== Gene Structure ===
+=== Gene Structure<ref name="ref2"/> ===
 [[File:5.png]]

@@ Line 38: / Line 38: @@
 transiently accumulated OsAns transcripts at around 3–6 h post-treatment. The control Nagina 22 plants did not show any detectable levels of OsAns transcripts. The OsDfr and OsAns transcripts accumulation was much higher under dehydration than that of high salt and ABA (Fig. 2). Both the OsDfr and OsAns transcripts reached the maximum by around 6 h post-treatment of dehydration and by about 3 h post-treatment of high salt, followed by a gradual decline to the basal level by 48 h. However, in case of the ABA treatment, the OsDfr transcript level reached to its maximum by 12 h, while that of the OsAns by 6 h after treatment, then declining back to the background level by 48 h.
-==== The rice regulatory gene, OsC1-myb, of the flavonoid pathway is responsive to abiotic stress ====
+==== The rice regulatory gene, OsC1-myb, of the flavonoid pathway is responsive to abiotic stress<ref name="ref1"/> ====
 We examined the effect of dehydration, high salt and ABA treatment on expression of the OsC1-myb homologues in rice seedlings. The OsC1-myb transcript which was not detectable in control plants, increased significantly up to 6 h and dropped down to background level by 48 h (Fig. 2). The pattern of expression of transcripts of the OsDfr, OsAns and OsC1-myb was similar in all the three treatment regimes. For each of the genes examined in these studies, the transcript levels remained constant in unstressed plants throughout the treatment period (data not shown).
-==== Promoter region of the OsDfr and the OsAns genes harbor multiple putative stress responsive elements ====
+==== Promoter region of the OsDfr and the OsAns genes harbor multiple putative stress responsive elements<ref name="ref1"/> ====
 To understand the correlated stress responsive expression of the OsDfr and the OsAns genes, the 5' upstream region of these two genes were analyzed in detail. Plant cis acting elements databases, PLACE and PlantCARE were searched using the OsDfr and the OsAns promoter sequences as a query to identify putative stress responsive motifs present in these two promoters. Visual inspection of the sequences was used to further characterize the domain organization in comparison with well-characterized and confirmed stress responsive regulatory domains. Putative sites of significance in stress response, identified in promoter region of the OsDfr and the OsAns, are listed in Table 2.
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 A MYB binding domain (TAACAAA) with a proximal pyramidine rich domain resembling the gibberellic acid responsive complex (GARC) of the rice and barley alpha-amylase gene promoter was found in 5' upstream region of the OsDfr gene (Fig. 3B). Also present on the OsDfr and the OsAns promoters is a domain sharing homology with the Arabidopsis Atmyb2 binding region (CAGTTA on the OsDfr and TGGTTAG on the OsAns) that is present in several drought stress responsive genes. Several putative ABRE/G-Box like elements with the central ACGT core are present in the promoter region of the OsAns gene (Table 2, Fig. 3C). A low temperature responsive element (LTRE) with a consensus sequence CCGACC flanked by ATGC like regions in the OsAns promoter was similar to that of the multiple stress responsive Arabidopsis cor 15a and the cold responsive Brassica BN115 genes promoter (Fig. 3C). Another interesting feature found in the OsAns promoter is the presence of a sph element, CACATGCATGCAC, (Fig. 3D).
-==== Putative myb responsive domains on OsDfr and OsAns promoter show differential binding affinities, in vitro, for the OsC1-MYB protein ====
+==== Putative myb responsive domains on OsDfr and OsAns promoter show differential binding affinities, in vitro, for the OsC1-MYB protein<ref name="ref1"/> ====
 To examine whether OsC1-MYB protein binds to different putative myb responsive domains identified in the OsDfr and the OsAns promoters, we used a recombinant 6His-OsC1-MYB fusion protein expressed in E. coli for protein–DNA binding studies. Gel mobility shift assay was used to study interaction between affinity purified recombinant OsC1-MYB protein and the PCR amplified oligonucleotide probes carrying different putative MYB binding domains.

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 The homozygous colorless mutant lines, Hamsa and Prasanna, are totally devoid of anthocyanin pigmentation or showed only trace amounts. The fully colored rice genotype, Purpleputtu, showed a significant increase in accumulation of anthocyanins under dehydration, high salt and ABA treatments, whereas the other two colored lines, R27 and G962, and the colorless Nagina 22 showed only a moderate increase. Dehydration stress appears to be more effective in triggering the anthocyanin accumulation among the competent genotypes.
-==== Stress responsive changes in expression of genes of the flavonoid pathway ====
+==== Stress responsive changes in expression of genes of the flavonoid pathway<ref name="ref1"/> ====
 We have analyzed stress responsive transcript accumulation of four genes of the phenyl propanoid and flavonoid pathways, OsPal (phenylalanine ammonialyase) OsChs (chalcone synthase), OsDfr (dihydroflavanol reductase) and OsAns (anthocyanidin synthase) under stress conditions. Northern analysis show that the transcript levels of the OsPal and OsChs genes were abundant in control seedlings and there were no significant changes upon stress treatment (Fig. 2).

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 ===Function===
-==== Anthocyanin accumulation in response to stress is genotype dependent ====
+==== Anthocyanin accumulation in response to stress is genotype dependent<ref name="ref1"/> ====
 Effect of dehydration, high salt and ABA on anthocyanin accumulation in different rice genotypes was analyzed. Ten-day-old seedlings were treated separately with 20%(w/v) PEG, 150mM NaCl and 100uM MABA and shoot samples were harvested 48 h after treatment. Anthocyanins were
 extracted in acidified methanol and spectrophotometrically quantitated as A530 value. The data revealed an enhanced anthocyanin accumulation in shoot tissues of competent rice genotypes (Fig. 1).

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 pathway genes in cooperation with a MYC family protein encoded by the R gene. The flavonoid biosynthetic pathway genes have been well-
 characterized and assigned with functions in two major cereal crop plants such as maize and rice. The rice flavonoid pathway is reported to
-respond to biotic and abiotic stress situations. Flavonoid accumulation in response to UV-B, cold, and drought were reported earilier.
+respond to biotic and abiotic stress situations. Flavonoid accumulation in response to UV-B, cold, and drought were reported earilier.<ref name="ref1"/>
 Drought and salinity tolerance in rice are two of the most important agronomic traits, and unraveling the genetic and molecular basis of
@@ Line 19: / Line 19: @@
 well-established extraction and purification protocols. Molecular dissection of the regulatory elements and the mechanisms by which they
 regulate the expression, temporally and spatially, of a host of genes belonging to diverse but functionally connected pathways will help in
-elucidation of the genetic basis of abiotic stress response in rice.
+elucidation of the genetic basis of abiotic stress response in rice.<ref name="ref1"/>
 ===Function===
 ==== Anthocyanin accumulation in response to stress is genotype dependent ====

@@ Line 1: / Line 1: @@
-''OsDfr'' is a stress-induced gene that produces dihydroflavonol-4-reductase involved in the flavonoid pathway.
+''OsDfr'' is a stress-induced gene that produces dihydroflavonol-4-reductase involved in the flavonoid pathway.<ref name="ref1"/>
 ==Annotated Information==
 ===Introduction===

← Older revision		Revision as of 13:40, 9 June 2014
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	<ref name="ref1">Nagabhushana Ithal, Arjula R. Reddy. (2004) Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB. Plant Science 166(6): 1505-1513</ref>		<ref name="ref1">Nagabhushana Ithal, Arjula R. Reddy. (2004) Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB. Plant Science 166(6): 1505-1513</ref>
	<ref name="ref2">[[http://rice.plantbiology.msu.edu/cgi-bin/ORF_infopage.cgi?orf=LOC_Os01g44260]]</ref>		<ref name="ref2">[[http://rice.plantbiology.msu.edu/cgi-bin/ORF_infopage.cgi?orf=LOC_Os01g44260]]</ref>
		+	</references>

← Older revision		Revision as of 13:39, 9 June 2014
Line 95:		Line 95:

	==References==		==References==
−	~~Please input cited~~ references ~~here~~.	+	<references>
		+	<ref name="ref1">Nagabhushana Ithal, Arjula R. Reddy. (2004) Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB. Plant Science 166(6): 1505-1513</ref>
		+	<ref name="ref2">[[http://rice.plantbiology.msu.edu/cgi-bin/ORF_infopage.cgi?orf=LOC_Os01g44260]]</ref>

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 ==Labs working on this gene==
 Please input related labs here.
 ==Structured Information==
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 === Gene Structure ===
 [[File:5.png]]