Os02g0220400 - Revision history

Xysj2012: /* Structured Information */

2015-05-14T05:36:10Z

‎Structured Information

Haihua2014 at 06:03, 11 June 2014

2014-06-11T06:03:00Z

Haihua2014 at 05:58, 11 June 2014

2014-06-11T05:58:58Z

Haihua2014 at 05:49, 11 June 2014

2014-06-11T05:49:29Z

Sw: /* Expression */

2014-05-19T15:20:58Z

‎Expression

Lymzzu2009 at 15:20, 19 May 2014

2014-05-19T15:20:19Z

Chengtao: /* Mutations */

2014-05-19T15:16:19Z

‎Mutations

Lymzzu2009 at 15:14, 19 May 2014

2014-05-19T15:14:40Z

Jiaohzh: /* References */

2014-05-19T15:09:30Z

‎References

Lymzzu2009 at 15:09, 19 May 2014

2014-05-19T15:09:03Z

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 The conserved GATA transcription factor Cga1 (Os02g12790) regulates chloroplast development and plant architecture. Strong overexpression of Cga1 produces dark green, semidwarf plants with reduced tillering, whereas RNA interference knockdown results in reduced chlorophyll and increased tillering. Coexpression, microarray, and real-time expression analyses demonstrate a correlation between Cga1 expression and the expression of important nucleus-encoded, chloroplast-localized genes. Constitutive Cga1 overexpression increases both chloroplast biogenesis and starch production but also results in delayed senescence and reduced grain filling<ref name="p7" />. It is one of transcription factors of the GATA family with a significant degree of conservation in the dicot model organism Arabidopsis<ref name="p1" />.
-Transgenic Arabidopsis plants with altered expression of CGA1 have been shown to exhibit differences in germination, chlorophyll content, chloroplast number, leaf size, flowering time, senescence and chloroplast biogenesis in cells<ref name="p4" /><ref name="p5" /><ref name="p6" /><ref name="p8" /><ref name="p9" />
+Transgenic Arabidopsis plants with altered expression of CGA1 have been shown to exhibit differences in germination, chlorophyll content, chloroplast number, leaf size, flowering time, senescence and chloroplast biogenesis in cells<ref name="p4" /><ref name="p5" /><ref name="p6" /><ref name="p8" /><ref name="p9" />.
 ===Mutations===

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 ===Function===
-Transgenic Arabidopsis plants with altered expression of ''CGA1'' have been shown to exhibit differences in germination, chlorophyll content, chloroplast number, leaf size, flowering time, and senescence<ref name="p4" /><ref name="p5" /><ref name="p6" />. This includes recent reports showing that ectopic overexpression promotes chloroplast biogenesis in cells where they are not typically found. These data indicate that GNC and CGA1 function as key transcriptional regulators of chloroplast biogenesis in Arabidopsis.
+The conserved GATA transcription factor Cga1 (Os02g12790) regulates chloroplast development and plant architecture. Strong overexpression of Cga1 produces dark green, semidwarf plants with reduced tillering, whereas RNA interference knockdown results in reduced chlorophyll and increased tillering. Coexpression, microarray, and real-time expression analyses demonstrate a correlation between Cga1 expression and the expression of important nucleus-encoded, chloroplast-localized genes. Constitutive Cga1 overexpression increases both chloroplast biogenesis and starch production but also results in delayed senescence and reduced grain filling<ref name="p7" />. It is one of transcription factors of the GATA family with a significant degree of conservation in the dicot model organism Arabidopsis<ref name="p1" />.
-A new research demonstrates that the conserved GATA transcription factor Cga1 (Os02g12790) regulates chloroplast development and plant architecture in rice (Oryza sativa)<ref name="p7" />.
+Transgenic Arabidopsis plants with altered expression of CGA1 have been shown to exhibit differences in germination, chlorophyll content, chloroplast number, leaf size, flowering time, senescence and chloroplast biogenesis in cells<ref name="p4" /><ref name="p5" /><ref name="p6" /><ref name="p8" /><ref name="p9" />
 ===Mutations===
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 <ref name="p6"> Hudson D, Guevara D, Yaish MW, Hannam C, Long N, Clarke JD, Bi YM, Rothstein SJ (2011) GNC and CGA1 modulate chlorophyll biosynthesis and glutamate synthase (GLU1/Fd-GOGAT) expression in Arabidopsis. PLoS ONE 6: e26765. </ref>
 <ref name="p7"> Hudson, D., Guevara, D. R., Hand, A. J., Xu, Z., Hao, L., Chen, X., Zhu, T., Bi, Y. M., and Rothstein, S. J. (2013). Rice cytokinin GATA transcription Factor1 regulates chloroplast development and plant architecture. Plant physiology 162, 132-144. </ref>
 </references>

← Older revision		Revision as of 05:49, 11 June 2014
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−	The CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR1/GNC-like (''CGA1/GNL'') transcription factor ~~was originally identified in Arabidopsis (At4g26150) due to rapidly increased expression following~~ cytokinin ~~application and similarity to a paralogous gene produced through genome duplication~~<ref name="p1" /><ref name="p2" /><ref name="p3" /><ref name="p4" />.	+	The CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR1/GNC-like (''CGA1/GNL'') transcription factor is the cytokinin-responsive GATA transcription factor<ref name="p1" /><ref name="p2" /><ref name="p3" /><ref name="p4" />.

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 15:20, 19 May 2014
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	===Expression===		===Expression===

		+	Rice tissues and patterns of expression for Cga1 were analyzed and established in wild-type Kaybonnet rice. Cga1 exhibited the strongest expression in green leaf tissue, with little and no expression in roots and floral organs, respectively (Figure3A).

		+	The expression of Cga1 following a number of treatments was also analyzed. Differences in Cga1 expression throughout the course of the day was also observed. (Figure3B). Light was found to significantly increase Cga1 expression, whereas periods of darkness reduced expression (Figure3, B and C). Cga1 was highly upregulated (approximately 5-fold) by the synthetic cytokinin benzyladenine (BA; figure3C). Nitrate (NO3 -) also significantly increased Cga1 expression, although to a lesser extent than BA (Figure3C).
		+
		+	[[File:Figure3lym.jpg]]
		+
		+	Figure 3. Expression of rice ''Cga1''(Os02g12790)<ref name="p7" />.Expression of rice ''Cga1'' (Os02g12790). A, Expression of Cga1 in selected rice tissues. B, Expression of Cga1 at 6-h intervals throughout the course of the day (long day; 16 h of light). C, Real-time PCR of ''OsCga1'' expression levels following periods of darkness or light and following treatment with 10 mM nitrate, 10 ''μ''mol of BA cytokinin, or 10 ''μ''mol of GA3.

	===Subcellular Localization===		===Subcellular Localization===

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 ===Mutations===
+Transgenic rice with altered expression of Cga1 exhibits differences in chlorophyll, chloroplast number, and starch content, which has also been reported in Arabidopsis. However, we also observed a dosage-dependent influence on phenotype, with strong overexpression causing a semidwarf phenotype, similar to the GA mutant Green Revolution varieties (Figure1).
 Novel evidence was presented that altering Cga1 expression in rice significantly influences tillering, biomass, and yield (Figure1, 2).

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 ==References==
+<references>
+<ref name="p1"> Reyes JC, Muro-Pastor MI, Florencio FJ (2004) The GATA family of transcription factors in Arabidopsis and rice. Plant Physiol 134: 1718–1732.</ref>
 ==Structured Information==