Os02g0274900 - Revision history

Xysj2012: /* Structured Information */

2015-05-14T05:38:30Z

‎Structured Information

Zhangye at 17:58, 21 December 2014

2014-12-21T17:58:42Z

Zhangye: /* Labs working on this gene */

2014-12-21T17:41:57Z

‎Labs working on this gene

Zhangye: /* Evolution */

2014-12-21T17:41:08Z

‎Evolution

Zhangye: /* Expression */

2014-12-21T17:29:08Z

‎Expression

Zhangye: /* Function */

2014-12-21T17:20:47Z

‎Function

Zhangye: /* Mutation */

2014-12-21T17:17:04Z

‎Mutation

Zhangye at 16:06, 21 December 2014

2014-12-21T16:06:30Z

Zhangye: /* References */

2014-12-21T15:47:23Z

‎References

Zhangye: /* Function */

2014-12-21T15:46:21Z

‎Function

@@ Line 62: / Line 62: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 Phylogenetic analysis (Fig. 1B) shows that the putative Arabidopsis paralogues to ''OsGMST1'' are encoded by ''At1g67300'', ''SGB1'', ''At1g05030'', and ''AtpGlcT'' and the proteins have 67%, 65%, 44%, and 43% identity to ''OsGMST1'',respectively.These four MST proteins are in the pGlcT subfamily. SGB1 Encodes a Putative Hexose Transporter.<ref name="ref3" /> In rice(''Japonica''), OsGMST1, Os09g23110, and Os01g04190(OspGlcT) belong to the pGlcT subfamily with sequence identity approximately 36%.SGB1 is shown here to be a Golgi-localized hexose transporter and acts genetically with AGB1 in early seedling development.<ref name="ref1" /><ref name="ref3" />.
 ==Labs working on this gene==
 *Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
@@ Line 49: / Line 54: @@
 * <ref name="ref4">
 Rolland F, Baena-Gonzalez E, Sheen J. Sugar sensing and signaling in plants: conserved and novel mechanisms[J]. Annu. Rev. Plant Biol., 2006, 57: 675-709.
 </ref>
 </references>

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 ==Labs working on this gene==
-Please input related labs here.
+*Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
 ==References==

@@ Line 26: / Line 26: @@
 * OsGMST1-GFP was co-expressed with the Golgi marker sialyltransferase (ST) tagged red fluorescence protein (RFP), most of the red and green fluorescence merged. The analysis by ''Hong Cao et al.'' suggests that ''OsGMST1'' is localized on the Golgi apparatus <ref name="ref1" />.
-===Evolution===
+===Homologues===
-Please input evolution information here.
+[[File:OsGMST1 3.png|right|thumb|200px|'''Figure 3.''' ''Phylogenic analysis of ''OsGMST1'' homologues from rice and other organisms by MEGA 4.0.(from reference) <ref name="ref1" />.'']]
+Phylogenetic analysis (Fig. 1B) shows that the putative Arabidopsis paralogues to ''OsGMST1'' are encoded by ''At1g67300'', ''SGB1'', ''At1g05030'', and ''AtpGlcT'' and the proteins have 67%, 65%, 44%, and 43% identity to ''OsGMST1'',respectively.These four MST proteins are in the pGlcT subfamily. SGB1 Encodes a Putative Hexose Transporter.<ref name="ref3" /> In rice(''Japonica''), OsGMST1, Os09g23110, and Os01g04190(OspGlcT) belong to the pGlcT subfamily with sequence identity approximately 36%.SGB1 is shown here to be a Golgi-localized hexose transporter and acts genetically with AGB1 in early seedling development.<ref name="ref1" /><ref name="ref3" />.
 ==Labs working on this gene==

@@ Line 16: / Line 16: @@
 ===Expression===
-Please input expression information here.
+[[File:OsGMST1 ExpressionPattern.jpg|left|thumb|300px|'''Figure 2.''' ''Expression analyses of ''OsGMST1''(from reference <ref name="ref1"/>).'']]
 ===Subcellular localization===

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 ''OsGMST1'' is a member of the monosaccharide transporter family in rice. It encodes a putative monosaccharide transporter.<ref name="ref1" /><br>Monosaccharide transporters play a pivotal role in the translocation and distribution of monosaccharides throughout the plant.The mobility of sugars between source and sink tissues in plants depends on sugar transport proteins.<ref name="ref1" /><ref name="ref2" /> ''Hong Cao et al.''speculates that this gene is a sugar transporter based on its sequence characteristics and the effect on the sugar content when the gene expression is reduced in rice.It is hypothesized that sugar homeostasis is affected in ''OsGMST1'' knockdown transgenic rice, which putatively results in altered sugar contents, so the seedlings show a NaCl hypersensitive phenotype.
-'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO: 0005215 GO: 0005215], [http://amigo.geneontology.org/amigo/term/GO: 0005351 GO: 0005351], [http://amigo.geneontology.org/amigo/term/GO: 0006810 GO: 0006810], [http://amigo.geneontology.org/amigo/term/GO: 0008643 GO: 0008643], [http://amigo.geneontology.org/amigo/term/GO: 0016020 GO: 0016020], [http://amigo.geneontology.org/amigo/term/GO: 0016021 GO: 0016021]
+'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0005215 GO:0005215], [http://amigo.geneontology.org/amigo/term/GO:0005351 GO:0005351], [http://amigo.geneontology.org/amigo/term/GO:0006810 GO:0006810], [http://amigo.geneontology.org/amigo/term/GO:0008643 GO:0008643], [http://amigo.geneontology.org/amigo/term/GO:0016020 GO:0016020], [http://amigo.geneontology.org/amigo/term/GO:0016021 GO:0016021]
 ===Mutation===

@@ Line 8: / Line 8: @@
 ===Mutation===
-[[File:OsGMST1_fig1.jpg|right|thumb|300px|''Salt tolerance test of ''OsGMST1'' antisense transgenic rice plants (from reference <ref name="ref1" />).'']]
+[[File:OsGMST1 fig1.png|right|thumb|300px|'''Figure 1.'''''Salt tolerance test of ''OsGMST1'' antisense transgenic rice plants (from reference <ref name="ref1" />).'']]
 *There were no differences in the seed germination rate between WT and the transgenic lines without NaCl stress(Figure 1A). Treatment with 150 mM and 200 mM NaCl,however, reduced the germination rate of AS-L12 and ASL18(Figure 1A, B).
 *At 200 mM NaCl, the germination rate of the wild type was 90%, while the germination rates of the AS-L12 and AS-L18 lines were reduced to 40% and 50%,respectively (P <0.01) (Figure 1B), and this difference was maintained over several days (P <0.01) (Figure 1C).

← Older revision		Revision as of 16:06, 21 December 2014
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	'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO: 0005215 GO: 0005215], [http://amigo.geneontology.org/amigo/term/GO: 0005351 GO: 0005351], [http://amigo.geneontology.org/amigo/term/GO: 0006810 GO: 0006810], [http://amigo.geneontology.org/amigo/term/GO: 0008643 GO: 0008643], [http://amigo.geneontology.org/amigo/term/GO: 0016020 GO: 0016020], [http://amigo.geneontology.org/amigo/term/GO: 0016021 GO: 0016021]		'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO: 0005215 GO: 0005215], [http://amigo.geneontology.org/amigo/term/GO: 0005351 GO: 0005351], [http://amigo.geneontology.org/amigo/term/GO: 0006810 GO: 0006810], [http://amigo.geneontology.org/amigo/term/GO: 0008643 GO: 0008643], [http://amigo.geneontology.org/amigo/term/GO: 0016020 GO: 0016020], [http://amigo.geneontology.org/amigo/term/GO: 0016021 GO: 0016021]
		+
		+	===Mutation===
		+	[[File:OsGMST1_fig1.jpg\|right\|thumb\|300px\|''Salt tolerance test of ''OsGMST1'' antisense transgenic rice plants (from reference <ref name="ref1" />).'']]
		+	*There were no differences in the seed germination rate between WT and the transgenic lines without NaCl stress(Figure 1A). Treatment with 150 mM and 200 mM NaCl,however, reduced the germination rate of AS-L12 and ASL18(Figure 1A, B).
		+	*At 200 mM NaCl, the germination rate of the wild type was 90%, while the germination rates of the AS-L12 and AS-L18 lines were reduced to 40% and 50%,respectively (P <0.01) (Figure 1B), and this difference was maintained over several days (P <0.01) (Figure 1C).
		+	*Reduced expression of OsGMST1 also conferredreduced tolerance to salt stress in young seedlings(Figure 1D).
		+	*The survival rate of AS-L12 (40% and 20% in 150 mM NaCl and 200 mM NaCl, respectively) and ASL18 (50% and 30%) were lower than the WT (70% and 50%)with treatment of 150 mM and 200 mM NaCl (P <0.01)(Figure 1E).
		+	*By contrast, the tolerance of OsGMST1 antisense plants to PEG and cold stress was the same as that of WT plants (Figure 1F, G). Just as for SGB1, increased expression of OsGMST1 had no effect.

	===Expression===		===Expression===

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 * <ref name="ref1">
 Cao H, Guo S, Xu Y, et al. Reduced expression of a gene encoding a Golgi localized monosaccharide transporter (OsGMST1) confers hypersensitivity to salt in rice (Oryza sativa)[J]. Journal of experimental botany, 2011: err178.
-</ref>.
+</ref>
-<references>
+* <ref name="ref2">
 ==Structured Information==

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-Please input function information here.
+''OsGMST1'' is a member of the monosaccharide transporter family in rice. It encodes a putative monosaccharide transporter.<ref name="ref1" /><br>Monosaccharide transporters play a pivotal role in the translocation and distribution of monosaccharides throughout the plant.The mobility of sugars between source and sink tissues in plants depends on sugar transport proteins.<ref name="ref1" /><ref name="ref2" /> ''Hong Cao et al.''speculates that this gene is a sugar transporter based on its sequence characteristics and the effect on the sugar content when the gene expression is reduced in rice.It is hypothesized that sugar homeostasis is affected in ''OsGMST1'' knockdown transgenic rice, which putatively results in altered sugar contents, so the seedlings show a NaCl hypersensitive phenotype.
 ===Expression===