Os02g0313400 - Revision history

Xysj2012: /* Structured Information */

2015-05-14T05:39:11Z

‎Structured Information

Zhaoxun13: /* Function */

2014-05-23T01:59:30Z

‎Function

Zhaoxun13: /* Function */

2014-05-23T01:54:43Z

‎Function

Zhaoxun13: /* Function */

2014-05-23T01:54:07Z

‎Function

Zhaoxun13: /* Function */

2014-05-23T01:53:36Z

‎Function

Zhaoxun13: /* Function */

2014-05-23T01:53:05Z

‎Function

Zhaoxun13: /* Function */

2014-05-23T01:52:49Z

‎Function

Zhaoxun13: /* Function */

2014-05-23T01:52:04Z

‎Function

Zhaoxun13: /* Evolution */

2014-05-23T01:50:50Z

‎Evolution

Zhaoxun13: /* Expression */

2014-05-23T01:28:17Z

‎Expression

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 Please input function information here.
 APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain(Figure1), results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5  interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development.
-[[File:zhao001.jpg]]
+[[File:zhao002.jpg]]
 ===Mutantion===

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 ===Function===
 Please input function information here.
-APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain(Figure1), results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5  interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development.[[File:zhao001.jpg]] '''Figure1.Amino acid sequence of API5 protein'''.The LXXLL motif is boxed. The nuclear localization signal is double underlined. The transactivation domain is underlined.
+APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain(Figure1), results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5  interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development.
 ===Mutantion===

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 ===Function===
 Please input function information here.
-APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain(Figure1), results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5  interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development.[[File:zhao001.jpg]]
+APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain(Figure1), results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5  interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development.[[File:zhao001.jpg]] '''Figure1.Amino acid sequence of API5 protein'''.The LXXLL motif is boxed. The nuclear localization signal is double underlined. The transactivation domain is underlined.
 ===Mutantion===

@@ Line 5: / Line 5: @@
 Please input function information here.
 APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain(Figure1), results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5  interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development.[[File:zhao001.jpg]]
-   '''Figure1.Amino acid sequence of API5 protein'''.
+   '''Figure1.Amino acid sequence of API5 protein'''.The LXXLL motif is boxed. The nuclear localization signal is double underlined. The transactivation domain is underlined.
 ===Mutantion===

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 Please input evolution information here.
-Make phylogenetic tree using API5 homologs from protists, plants, and animals. 32 API5 protein sequences were classified into three clusters according to the
+API5 proteins exist in the majority of eukaryotes Make phylogenetic tree using API5 homologs from protists, plants, and animals. 32 API5 protein sequences were classified into three clusters according to the phylogenetic tree (Figure 6). There are three major groups in the phylogenetic analyses may indeed reflect the systematization of animals, plants, and protists.
 [[File:zhao5.jpg]]

← Older revision		Revision as of 01:28, 23 May 2014
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	Using transgenic lines harboring the full-length coding sequence (CDS) of Os API5 driven by the maize Ubiquitin promoter in the genetic background of japonica cultivar Zhonghua11 to gain more knowledge regarding the function of API5. The relative expression level of API5 between transgene-positive and -negative plants of the T1 generation derived from a single copy transgenic T0 plant was verified by qRT-PCR. The amount of API5 transcripts in positive plants was significantly enhanced (>80-fold) compared with negative plants . The normal fertility of both positive and negative plants suggested that the development of reproductive organs was normal in API5-overexpressing plants .The morphology of the positive plants relative to their negative counterparts at the seedling and reproductive stages, and also the cytologic characteristics of roots, culms, and leaves were no obvious differences between them.		Using transgenic lines harboring the full-length coding sequence (CDS) of Os API5 driven by the maize Ubiquitin promoter in the genetic background of japonica cultivar Zhonghua11 to gain more knowledge regarding the function of API5. The relative expression level of API5 between transgene-positive and -negative plants of the T1 generation derived from a single copy transgenic T0 plant was verified by qRT-PCR. The amount of API5 transcripts in positive plants was significantly enhanced (>80-fold) compared with negative plants . The normal fertility of both positive and negative plants suggested that the development of reproductive organs was normal in API5-overexpressing plants .The morphology of the positive plants relative to their negative counterparts at the seedling and reproductive stages, and also the cytologic characteristics of roots, culms, and leaves were no obvious differences between them.