Os02g0491300 - Revision history

Xysj2017 at 09:07, 14 February 2017

2017-02-14T09:07:35Z

Xysj2017: /* Annotated Information */

2017-02-14T09:07:09Z

‎Annotated Information

Xysj2017 at 09:06, 14 February 2017

2017-02-14T09:06:56Z

Xysj2017: /* Annotated Information */

2017-02-14T09:05:22Z

‎Annotated Information

Xysj2017: /* Mutation and Phenotype */

2017-02-14T09:03:40Z

‎Mutation and Phenotype

Xysj2017 at 09:03, 14 February 2017

2017-02-14T09:03:23Z

Xysj2017: /* Annotated Information */

2017-02-14T09:00:20Z

‎Annotated Information

Xysj2017 at 09:00, 14 February 2017

2017-02-14T09:00:10Z

Xysj2017 at 08:59, 14 February 2017

2017-02-14T08:59:06Z

Xysj2017: /* Gene Structure */

2017-02-14T08:57:38Z

‎Gene Structure

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 ==Annotated Information==
 [[File:221-Os02g0491300.png|right|thumb|427px|'''Figure 1.''' ''Phenotypic Analysis of mtr1 Anthers.<ref name="ref1" />.'']]
 ===Function===

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 ===Gene Structure===
 * ''MTR1'' locus was located on chromosome 2 between two InDel markers, TH239 and TH234. The MTR1 transcript is approximately 2 kb in length and contains a 1,752 bp coding sequence without any intron, a 55 bp 5' untranslated region (UTR) and a 193 bp 3' UTR.
 * The predicted MTR1 protein is 583 amino acids long and contains a putative N-terminal signal peptide; two fasciclin domains (amino acids 257–356 and 435–535); and two potential N-glycosylation sites (amino acids 433 and 554).
+[[File:MTR4.jpg|right|thumb|427px|'''Figure 6.''' ''Map-Based Cloning analysis of MTR1<ref name="ref1" />.'']]
 ===Mutation and Phenotype===
 * mtr1 shows normal vegetative and nonreproductive floral organ development, but has smaller and pale-yellow anthers that fail to produce viable pollen grains (Figures S1A–S1F available online; Figures 1A and 1B).

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:221-Os02g0491300.jpg|right|thumb|427px|'''Figure 1.''' ''Phenotypic Analysis of mtr1 Anthers.<ref name="ref1" />.'']]
+[[File:221-Os02g0491300.png|right|thumb|427px|'''Figure 1.''' ''Phenotypic Analysis of mtr1 Anthers.<ref name="ref1" />.'']]
 ===Function===
 * MICROSPORE AND TAPETUM REGULATOR1 ('''''MTR1''''') is a rice fascilin glycoprotein protein.

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:MTR4.jpg|right|thumb|427px|'''Figure 6.''' ''Map-Based Cloning analysis of MTR1<ref name="ref1" />.'']]
+[[File:221-Os02g0491300.jpg|right|thumb|427px|'''Figure 1.''' ''Phenotypic Analysis of mtr1 Anthers.<ref name="ref1" />.'']]
 ===Function===
 * MICROSPORE AND TAPETUM REGULATOR1 ('''''MTR1''''') is a rice fascilin glycoprotein protein.
@@ Line 12: / Line 12: @@
 ===Gene Structure===
 * ''MTR1'' locus was located on chromosome 2 between two InDel markers, TH239 and TH234. The MTR1 transcript is approximately 2 kb in length and contains a 1,752 bp coding sequence without any intron, a 55 bp 5' untranslated region (UTR) and a 193 bp 3' UTR.
 * The predicted MTR1 protein is 583 amino acids long and contains a putative N-terminal signal peptide; two fasciclin domains (amino acids 257–356 and 435–535); and two potential N-glycosylation sites (amino acids 433 and 554).
 ===Mutation and Phenotype===

@@ Line 19: / Line 19: @@
 * mtr1 shows normal vegetative and nonreproductive floral organ development, but has smaller and pale-yellow anthers that fail to produce viable pollen grains (Figures S1A–S1F available online; Figures 1A and 1B).
 * All the F1 progeny from the backcross between wild-type and mtr1 displayed the wide-type phenotype, and the F 2 progeny yielded a segregation of 481 wild-type and 147 mutant plants (c 2 = 0.85,p > 0.05), suggesting a monofactorial recessive inheritance of the mutation.
-* To characterize the defects of anther development in mtr1, researchers performed 4 0 ,6-diamidino-2-phenylindole (DAPI) staining of
+* To characterize the defects of anther development in mtr1, researchers performed 4 0 ,6-diamidino-2-phenylindole (DAPI) staining of microspores.
 # At stage 8b of anther development, mtr1 produced normal tetrads just like the wild-type (Figures 1C and 1D). However, at stages 9 and 10, after the microspores were released from the tetrad, mtr1 microspores expanded much more slowly than those of the wild-type (Figures 1E–1H).
 # At later stages, each wild-type microspore underwent mitosis, gener- ating the mature trinucleate pollen grain that contains two smaller sperm nuclei and a larger vegetative nucleus (Figures 1I and 1K).

@@ Line 17: / Line 17: @@
 ===Mutation and Phenotype===
-Please input Mutation and Phenotype information here.
+* mtr1 shows normal vegetative and nonreproductive floral organ development, but has smaller and pale-yellow anthers that fail to produce viable pollen grains (Figures S1A–S1F available online; Figures 1A and 1B).
+* All the F1 progeny from the backcross between wild-type and mtr1 displayed the wide-type phenotype, and the F 2 progeny yielded a segregation of 481 wild-type and 147 mutant plants (c 2 = 0.85,p > 0.05), suggesting a monofactorial recessive inheritance of the mutation.
-''mtr1'' shows normal vegetative and nonreproductive floral organ development, but has smaller and pale-yellow anthers that fail to produce viable pollen grains.(A: wiletype; B: ''mtr1'')
+* To characterize the defects of anther development in mtr1, researchers performed 4 0 ,6-diamidino-2-phenylindole (DAPI) staining of
+microspores.
-[[File:MTR1.jpg]]
+# At stage 8b of anther development, mtr1 produced normal tetrads just like the wild-type (Figures 1C and 1D). However, at stages 9 and 10, after the microspores were released from the tetrad, mtr1 microspores expanded much more slowly than those of the wild-type (Figures 1E–1H).
+# At later stages, each wild-type microspore underwent mitosis, gener- ating the mature trinucleate pollen grain that contains two smaller sperm nuclei and a larger vegetative nucleus (Figures 1I and 1K).
-The result of (DAPI) stain show that start from stage 9 ''mtr1'' microspores  show slow development, did not seem to go through mitosis, and were eventually aborted. Transverse section analysis showe ''mtr1'' anther wall layers were less degenerated than wild-type and microspores were collapsed.transmission electron microscopy (TEM) and scanning electron microscopy (SEM) of the anther show ''mtr1'' tapetal cells were highly vacuolated and microspores were irregularly shaped with less deposition of sporopollenin precursors on the surface. And, at stage 7 and 8b, when wild-type tapetal cells showed TUNEL-positive nuclei. However, the ''mtr1'' tapetal cells had no detectable TUNEL signals. Several rice tapetum-expressed genes, which are known to be involved in tapetal function and degeneration, displayed abnormal expression patterns in ''mtr1'' .
+# By contrast, mtr1 microspores continued to show slow development, did not seem to go through mitosis, and were eventually aborted (Figures 1J and 1L).
 ==Labs working on this gene==
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 </ref>
 </references>
 ==Structured Information==