Os02g0527600 - Revision history

Xysj1986: /* Phenotypic analysis */

2016-10-10T05:16:58Z

‎Phenotypic analysis

2016-10-10T05:16:07Z

‎Expression

2016-10-10T05:15:38Z

‎Evolution

2016-10-10T05:15:25Z

‎Expression

2016-10-10T05:15:12Z

2016-10-10T05:14:53Z

2016-10-10T05:14:36Z

‎Annotated Information

2016-10-10T05:13:01Z

‎References

2016-10-10T05:12:18Z

‎Labs working on this gene

2016-10-10T05:11:54Z

‎Expression

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 ===Phenotypic analysis===
 * To evaluate whether '''''OsCTR1''''' and '''''OsCTR2''''' have any roles in ethylene-receptor signalling, the researchers ectopically expressed each gene in a ctr1-1 loss-of-function mutant for complementation testing.
-* The expression of '''''OsCTR2''''' partially rescued the ctr1-1 ethylene response phenotype. Light-grown ctr1-1 seedlings produced small, compact cotyledons and a short root; consistently, ctr1-1 transgenic lines expressing CTR1p:OsCTR2 produced larger cotyledons and a longer root than ctr1-1 seedlings (Fig.  2E). At the adult stage, expression of the CTR1p:OsCTR2 transgene largely rescued the ctr1-1 growth inhibition phenotype, and the transgenic lines produced a normal rosette, as in wild-type plants (Col-0)
+* The expression of '''''OsCTR2''''' partially rescued the ctr1-1 ethylene response phenotype. Light-grown ctr1-1 seedlings produced small, compact cotyledons and a short root; consistently, ctr1-1 transgenic lines expressing CTR1p:OsCTR2 produced larger cotyledons and a longer root than ctr1-1 seedlings (Fig.  2E).
 ===Subcellular Localization ===

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 * The expression of '''''OsCTR2''''' partially rescued the ctr1-1 ethylene response phenotype. Light-grown ctr1-1 seedlings produced small, compact cotyledons and a short root; consistently, ctr1-1 transgenic lines expressing CTR1p:OsCTR2 produced larger cotyledons and a longer root than ctr1-1 seedlings (Fig.  2E). At the adult stage, expression of the CTR1p:OsCTR2 transgene largely rescued the ctr1-1 growth inhibition phenotype, and the transgenic lines produced a normal rosette, as in wild-type plants (Col-0)
-===Expression===
+===Subcellular Localization ===
 * Researchers have already studied the subcellular localization of '''''OsCTR2''''' with GFP-fused OsCTR2.
 * Green (GFP–OsCTR2) and red (ER-rk) fluorescence was observed mainly surrounding the nucleus, at the cell edge of tobacco cells, with an uneven, discrete pattern (Fig. 5A), and the ER network was also observed (Fig. 5B).

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 * To obtain other evidence supporting localization of '''''OsCTR2''''' at the ER, GFP–OsCTR2 was transiently co-expressed with ER-rk in onion epidermal cells by particle bombardment. Consistently, the fluorescence of GFP–OsCTR2 and ER-rk co-localized (Fig. 5C). The nucleus was surrounded by GFP–OsCTR2 and ER-rk (Fig. 5D), which is consistent with the close association of the ER with the outer membrane of the nuclear envelope.
-===Evolution===
 You can also add sub-section(s) at will.

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 ===Expression===
 * Researchers have already studied the subcellular localization of '''''OsCTR2''''' with GFP-fused OsCTR2.
-* Green (GFP–OsCTR2) and red (ER-rk) fluorescence was observed mainly surrounding the nucleus, at the cell edge of tobacco cells, with an uneven, discrete pattern
+* Green (GFP–OsCTR2) and red (ER-rk) fluorescence was observed mainly surrounding the nucleus, at the cell edge of tobacco cells, with an uneven, discrete pattern (Fig. 5A), and the ER network was also observed (Fig. 5B).
 * To obtain other evidence supporting localization of '''''OsCTR2''''' at the ER, GFP–OsCTR2 was transiently co-expressed with ER-rk in onion epidermal cells by particle bombardment. Consistently, the fluorescence of GFP–OsCTR2 and ER-rk co-localized (Fig. 5C). The nucleus was surrounded by GFP–OsCTR2 and ER-rk (Fig. 5D), which is consistent with the close association of the ER with the outer membrane of the nuclear envelope.

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 The rice '''''Os02g0527600''''' was reported as ''''' OsCTR2 ''''' in 2013 <ref name="ref1" /> by researchers from China.
 ==Annotated Information==
 ===Gene Symbol===
 *'''''Os02g0527600''''' '''''<=>''''' '''''OsCTR2''''','''''CTR2'''''

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 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==
       [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]

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 ==Labs working on this gene==
-Please input related labs here.
+* National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China
 ==References==