Os02g0552700 - Revision history

Xysj2028 at 12:35, 8 May 2017

2017-05-08T12:35:31Z

Xysj2028: /* References */

2017-05-08T12:25:54Z

‎References

Xysj2028: /* Labs working on this gene */

2017-05-08T12:24:34Z

‎Labs working on this gene

Xysj2028: /* Labs working on this gene */

2017-05-08T12:24:24Z

‎Labs working on this gene

Xysj2028: /* Labs working on this gene */

2017-05-08T12:24:06Z

‎Labs working on this gene

Xysj1981 at 06:22, 8 March 2017

2017-03-08T06:22:12Z

Xysj2012: /* Structured Information */

2015-05-14T05:43:00Z

‎Structured Information

Nigel9011: /* Function */

2014-06-10T05:25:20Z

‎Function

Nigel9011: /* Annotated Information */

2014-06-10T05:10:35Z

‎Annotated Information

Nigel9011: /* Annotated Information */

2014-06-10T05:05:17Z

‎Annotated Information

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-Please input one-sentence summary here.
+The rice '''''Os06g0493100''''' was reported as '''''OsZFP6''''' in 2014 <ref name="ref1" /> by researchers from China.
 ==Annotated Information==
 ===Function===
-This gene encodes a novel CCHC-type zinc finger pronein called OsZFP6. OsZFP6 is structurally and functionally conserved, mainly localised in nucleus, and can play an important role in stress tolerance responses to salt (NaCl), alkali (NaHCO<sub>3</sub>), and H<sub>2</sub>O<sub>2</sub> treatment in rice. Functional analysis also showed overexpression of this gene in transgenic <i>Arabidopsis</i> and yeast to NaHCO<sub>3</sub> and H<sub>2</sub>O<sub>2</sub>.
+* '''''OsZFP6''''' is a putatively useful gene for developing crops with increased alkali and H2O2 tolerance.
-Fig. 1. <i>OsZFP6</i> expression after treatment with 150 mM NaCl, 60 mM NaHCO<sub>3</sub>, or 5 mM H<sub>2</sub>O<sub>2</sub> for 0, 12, 24, and 48 h, respectively. Bars with different letters showed significant differences at P < 0.01 (Means ± SD, n = 6).
+===Phenotypic analysis===
+* When OsZFP6 was transformed into yeast, the transgenic yeast showed significantly increased resistance to NaHCO3 compared to the control. * Moreover, Arabidopsis transgenic plants overexpressing ''''OsZFP6''''' were more tolerant to both NaHCO3 and H2O2 treatments.
 ===Expression===
-Using the online analysis tool Psort to predict the subcellular localisation of rice OsZFP6, OsZFP6 was localised to the nucleus with a 73.9% probability. To validate the predicted result, a PBI121-<i>OsZFP6</i>:<i>GFP</i> plasmid was transformed into onion epidermal cells. After culture for 18-24 h in the dark, green fluorescence was observed in the nucleus (as shown in figure), suggesting the OsZFP6 protein, driven by the 35S promoter, was localized in the nucleus.
+* '''''OsZFP6''''' expression was increased by abiotic stress, including salt (NaCl), alkali (NaHCO 3 ) and H2O2 treatment
-===Evolution===
+===Subcellular localization===
-The CysCysHisCys (CCHC)-type zinc finger domains contain the sequence C-X2-C-X4-H-X4-C, where X can be any amino acid, and the numbers indicate the number of residues. This domain is conserved in <i>Arabidopsis</i>, yeast and rice, as shown in table.
+* The '''''OsZFP6''''' protein contains 305 amino acids and a conserved zinc finger domain and is localised to the nucleus.
 ==Labs working on this gene==
-* Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, Heilongjiang 150081, China
+* Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin,Heilongjiang 150081, China
 * Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan 571737, China
-* Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, Heilongjiang 150040, China
+* Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, Heilongjiang 150040, China College of Life Sciences, Xinyang Normal University, China.
 ==References==
 <references>

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 ==References==
-Guan QJ, Wang LF, Bu QY, Wang ZY. (2014) The rice gene <i>OsZFP6</i> functions in multiple stress tolerance responses in yeast and <i>Arabidopsis</i>. Plant Physiol Bioch 82: 1-8.
+<references>
 ==Structured Information==

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 ==Labs working on this gene==
 * Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, Heilongjiang 150081, China
-* Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and
+* Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan 571737, China
 * Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, Heilongjiang 150040, China

@@ Line 19: / Line 19: @@
 ==Labs working on this gene==
-* Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin,
+* Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, Heilongjiang 150081, China
 * Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and
 Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan 571737, China

@@ Line 19: / Line 19: @@
 ==Labs working on this gene==
-Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, Heilongjiang 150081, China.
+* Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin,
 ==References==

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 [[File:Os02g0552700-4.jpg]]
-Fig. 1. <i>OsZFP6</i> expression after treatment with 150 mM NaCl, 60 mM NaHCO<sub>3</sub>, or 5 mM H<sub>2</sub>O<sub>2</sub> for 0, 12, 24, and 48 h, respectively. Bars with different letters showed significant differences at P &#60 0.01 (Means  &#177 SD, n = 6).
+Fig. 1. <i>OsZFP6</i> expression after treatment with 150 mM NaCl, 60 mM NaHCO<sub>3</sub>, or 5 mM H<sub>2</sub>O<sub>2</sub> for 0, 12, 24, and 48 h, respectively. Bars with different letters showed significant differences at P < 0.01 (Means ± SD, n = 6).
 ===Construction===

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 [[File:Os02g0552700-4.jpg]]
 ===Construction===
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 [[File:Os02g0552700-1.jpg]]
 [[File:Os02g0552700-3.jpg]]
 ===Evolution===
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 [[File:Os02g0552700-2.jpg]]
 ==Labs working on this gene==