Os02g0620600 - Revision history

192.168.72.52 at 06:43, 14 May 2015

2015-05-14T06:43:16Z

LIZHAO: /* Evolution */

2014-06-10T03:46:44Z

‎Evolution

LIZHAO: /* References */

2014-06-10T03:41:07Z

‎References

LIZHAO: /* References */

2014-06-10T03:40:27Z

‎References

LIZHAO: /* Evolution */

2014-06-10T03:38:21Z

‎Evolution

LIZHAO: /* Expression */

2014-06-10T03:36:18Z

‎Expression

LIZHAO: /* Expression */

2014-06-09T15:16:16Z

‎Expression

LIZHAO: /* Expression */

2014-06-09T15:15:23Z

‎Expression

LIZHAO: /* Expression */

2014-06-09T15:10:21Z

‎Expression

LIZHAO: /* Expression */

2014-06-09T15:08:42Z

‎Expression

← Older revision		Revision as of 06:43, 14 May 2015
Line 47:		Line 47:

	==Structured Information==		==Structured Information==
−	~~{{JaponicaGene\|~~	+	[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]
−	~~GeneName = Os02g0620600\|~~
−	~~Description = Ammonium transporter family protein\|~~
−	~~Version = NM_001053991.1 GI:115447352 GeneID:4330008\|~~
−	~~Length = 1853 bp\|~~
−	~~Definition = Oryza sativa Japonica Group Os02g0620600, complete gene.\|~~
−	~~Source = Oryza sativa Japonica Group~~
−
−	~~ORGANISM Oryza sativa Japonica Group~~
−	~~Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;~~
−	~~Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP~~
−	~~clade; Ehrhartoideae; Oryzeae; Oryza.~~
−	\|
−	~~Chromosome = [[:category:Japonica Chromosome 2\|Chromosome 2]]\|~~
−	~~AP = Chromosome 2:25548745..25550597\|~~
−	~~CDS = 25548816..25550306\|~~
−	~~GCID = <gbrowseImage1>~~
−	~~name=NC_008395:25548745..25550597~~
−	~~source=RiceChromosome02~~
−	~~preset=GeneLocation~~
−	~~</gbrowseImage1>\|~~
−	~~GSID = <gbrowseImage2>~~
−	~~name=NC_008395:25548745..25550597~~
−	~~source=RiceChromosome02~~
−	~~preset=GeneLocation~~
−	~~</gbrowseImage2>\|~~
−	CDNA = <cdnaseq>atggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctag</cdnaseq>\|
−	AA = <aaseq>MATCADTLGPLLGTAAANATDYLCNQFADTTSAVDSTYLLFSAY LVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIG KHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVY PVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIG RFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAV GRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAI ICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQ PGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGM DQTRHGGFAYAYHDDDASGKPDRSVGGFMLKSAHGTQVAAEMGGHV</aaseq>\|
−	DNA = <dnaseqindica>72..1562#acagcccacacttccattgctcctcccctctcctctacagtctgtgttgagcgcgcgtcgaggcggcgaggatggcaacgtgcgcggataccctcggcccgctgctgggcacggcggcggcgaacgcgacggactacctgtgcaaccagttcgcggacacgacgtcggccgtggactcgacgtacctgctcttctcggcgtacctcgtgttcgccatgcagctcggcttcgccatgctctgcgccgggtccgtccgcgccaagaacaccatgaacatcatgcttaccaacgtgctcgacgccgccgccggcgcgctcttctactacctcttcggcttcgccttcgccttcggggcgccgtccaacggcttcatcgggaagcacttcttcggcctcaagcaggtcccacaggtcggcttcgactacagcttcttcctcttccagtgggccttcgccatcgccgccgcgggcatcacgtccggctccatcgccgagcggacccagttcgtggcgtacctcatctactccgccttcctcaccggcttcgtctacccggtggtgtcccactggatctggtccgccgacgggtgggcctcggcttcccggacgtcggggtcgctgctcttcgggtccggcgtcatcgacttcgccgggtcaggggttgtccacatggtgggcggcgtggccggactctggggcgccctcatcgagggcccccgcattgggcggttcgaccacgccggccgctcggtggcgctgcgcggccacagcgcgtcgctcgtcgtgctcggcagcttccttctgtggttcgggtggtacgggtttaaccccggctcgttcctcaccatcctcaaatcctacggcccgcccggtagcatccacgggcagtggtcggcggtgggacgcaccgccgtgaccaccaccctcgccggcagcacggcggcgctcacgacgctcttcgggaagaggctccagacggggcactggaacgtgatcgacgtctgcaacggcctcctcggcggcttcgcggcgatcaccgccggttgctccgtcgtcgacccgtgggccgcgatcatctgcgggttcgtctcggcgtgggtgctcatcggcctcaacgcgctggcggcgaggctcaagttcgacgacccgctcgaggcggcgcagctgcacggcgggtgcggcgcgtggggggtcatcttcacggcgctgttcgcgcgcaaggagtacgtggaccagatcttcggccagcccgggcgcccgtacgggctgttcatgggcggcggcggccggctgctcggggcgcacatagtggtcatcctggtcatcgcggcgtgggtgagcttcaccatggcgccgctgttcctggtgctcaacaagctgggcttgctgcgcatctcggccgaggacgagatggccggcatggaccagacgcgccacggcgggttcgcgtacgcgtaccacgacgacgacgcgagcggcaagccggaccgcagcgtcggcgggttcatgctcaagtcggcgcacggcacgcaggtcgccgccgagatgggaggccatgtctagtggaaccggaggagctgagctagtagtacatacatgcagcatcatcgatcgaacgaaatgcatataagcgtttttcaaggttgatctgatgctgcaggtttcgtgattgtataataggaagaaaaagatagtagtattttttatctgagatcatctgtttggaacaggggatttgactaagatttgatataaatttacacaaaatcttagcaaaaatccctttatctcaactctcaagtagagctttgctttgtacaacaaagtatcatgtgtgatataattgtcaggtgg</dnaseqindica>\|
−	~~Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053991.1 RefSeq:Os02g0620600]\|~~
−	}}
−	[[Category:Genes]]
−	~~[[Category:Japonica mRNA]]~~
−	[[Category:Oryza Sativa Japonica Group]]
−	[[Category:Japonica ~~Genes]]~~
−	~~[[Category:Japonica Chromosome 2]]~~
−	~~[[Category:~~Chromosome 2]]

@@ Line 21: / Line 21: @@
 # Diversity and selective sweep in the OsAMT1;2 genomic region of rice. AMT1;2 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions.
 # OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').<ref name="ref1" />
-[[File:LIZHAO4.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.]]
+[[File:LIZHAO4.png‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.]]
 # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').<ref name="ref5" />
-[[File:LIZHAO5.jpg‎|frame|'''Figure5''']]
+[[File:LIZHAO5.png‎|frame|'''Figure5''']]
 ==Labs working on this gene==

@@ Line 38: / Line 38: @@
 ==References==
 <ref name="ref1">Sonoda Y, Ikeda A, Yamaya T, et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice[C]//Plant and Cell Physiology. GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS, 2004, 45: S98-S98.</ref>
 <ref name="ref2">Suenaga A, Moriya K, Sonoda Y, et al. Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants[J]. Plant and Cell Physiology, 2003, 44(2): 206-211.</ref>
@@ Line 43: / Line 44: @@
 <ref name="ref4">Lauter F.R. O. Ninnemann, M. Bucher, J.W. Riesmeier and W.B. Frommer, 1996. Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato[C]//Proc. Natl. Acad. Sci. USA 93: 8139-8144. </ref>
 <ref name="ref5">Y. SONODA, A. IKEDA and J. YAMAGUCHI Characterization of rice ammonium transporters, OsAMT1;1 and OsAMT1;2[C]// Rice Genetics Newsletter, Vol. 17.</ref>
 ==Structured Information==

@@ Line 38: / Line 38: @@
 ==References==
-Please input cited references here.
+<ref name="ref1">Sonoda Y, Ikeda A, Yamaya T, et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice[C]//Plant and Cell Physiology. GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS, 2004, 45: S98-S98.</ref>
 ==Structured Information==

@@ Line 19: / Line 19: @@
 ===Evolution===
-# OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').<ref name="ref4" />
+# Diversity and selective sweep in the OsAMT1;2 genomic region of rice. AMT1;2 transgenic rice plants with enhanced NH4+ permeability show superior growth and higher yield under optimal and suboptimal NH4+ conditions.
-[[File:LIZHAO.jpg‎|frame|'''Figure4'''Localization of OsAMT1;2mRNA in rice root tip by in situ hybridization.
+# OsAMT1;2（Os02g0620500）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3). OsAMT1s belong to AMT1 family, containing 11 putative trans-membrane-spanning domains. ('''Figure4''').<ref name="ref1" />
 # Hydropathy analysis of the deduced amino acid sequence suggests that OsAMT1;1 and OsAMT1;2 contain 10-11 putative trans-membrane domains. Sequence comparison shows that OsAMT1;1 and OsAMT1;2 are closely related to Arabidopsis and tomato ammonium transporters (67-63%,). ('''Figure5''').<ref name="ref5" />
-[[File:LIZHAO.jpg‎|frame|'''Figure5'''
+[[File:LIZHAO5.jpg‎|frame|'''Figure5''']]
 ==Labs working on this gene==

@@ Line 10: / Line 10: @@
 ===Expression===
 # OsAMT1;2 is expressed in the central cylinder and cell surface of root tips.
-# OsAMT1;2 expression was root specific and ammonium inducible.
+# OsAMT1;2 expression was root specific and ammonium inducible.<ref name="ref2" />
-# Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.
+# Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand,glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').<ref name="ref3" />[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]
-# Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').<ref name="ref4" />[[File:LIZHAO1.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]
+OsAMT1gene expression is positively feedback-regulated (dotted arrow) and negatively feedback-regulated (dotted T line) by cytosolic glutamine(Gln). Nitrogen fluxes are shown as solid arrows. See text for details.
-while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 ).<ref name="ref2" />[[File:LIZHAO2.png‎|frame|'''Figure 1'''while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).]]
 ===Evolution===

@@ Line 13: / Line 13: @@
 # Ammonium showed positive effects on the expression of OsAMT1;2. These effects were mimicked not only by glutamine but also by asparagines. On the other hand.
 # Glutamate and aspartate were ineffective with respect to OsAMT1;2.('''Figure 1''').<ref name="ref4" />[[File:LIZHAO.png‎|frame|'''Figure 1'''Hypothetical model of the regulation of OsAMT1 genes in rice roots.]]
-while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).<ref name="ref2" />
+while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 ).<ref name="ref2" />[[File:LIZHAO.png‎|frame|'''Figure 1'''while OsAMT1;1 was constitutively expressed (Figs. 2 and 3), accumulation of OsAMT1;2 transcripts was roots-specific and ammonium-inducible (Figs. 2 and 3).]]
 ===Evolution===